Quantitative analysis of G-protein-coupled receptor internalization using DnaE intein-based assay

Lü, Bin; Chen, Linjie; Zhang, Yaping; Shi, Ying; Zhou, Naiming

doi:10.1016/bs.mcb.2015.10.006

Methods in Cell Biology

2016

DOI: 10.1016/bs.mcb.2015.10.006

|View full text |Cite

Quantitative analysis of G-protein-coupled receptor internalization using DnaE intein-based assay

Bin Lü¹,

Linjie Chen²,

Yaping Zhang³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2019

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Biotechnological Applications of Protein Splicing

Sarmiento

Camarero

2019

CPPS

View full text Add to dashboard Cite

Protein splicing domains, also called inteins, have become a powerful biotechnological tool for applications involving molecular biology and protein engineering. Early applications of inteins focused on self-cleaving affinity tags, generation of recombinant polypeptide α-thioesters for the production of semisynthetic proteins and backbone cyclized polypeptides. The discovery of naturallyoccurring split-inteins has allowed the development of novel approaches for the selective modification of proteins both in vitro and in vivo. This review gives a general introduction to protein splicing with a focus on their role in expanding the applications of intein-based technologies in protein engineering and chemical biology.

show abstract

Biotechnological Applications of Protein Splicing

Sarmiento

Camarero

2019

CPPS

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Quantitative analysis of G-protein-coupled receptor internalization using DnaE intein-based assay

Cited by 1 publication

References 54 publications

Biotechnological Applications of Protein Splicing

Biotechnological Applications of Protein Splicing

Contact Info

Product

Resources

About