Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)

Yang, Zhuo; Choi, Ji Sun; Marin, Thibault; Yu, Hwanjo; Harley, Brendan A.C.; Cunningham, Brian T.

doi:10.1038/s41377-018-0001-5

Cited by 20 publications

(17 citation statements)

References 84 publications

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“…These results demonstrate that the many attractive features of resonant hyperspectral imaging for studies involving e.g. adhesion, secretion and cell viability previously demonstrated with mammalian cells [21,22,44] can now also be applied to bacteria.…”

Section: Single Bacteria Detection With Resonant Hyperspectral Imagingsupporting

confidence: 52%

“…Two major aspects have so far been missed, however. a) The dynamic range of the resonance is essential for a high signal-to-noise measurement, yet much of the previous work has aimed to maximise the Q-factor alone; b) The high sensitivity can also be most fruitfully applied to imaging applications, which so far has been limited to mammalian cells due to their larger (5-10 µm) size [9,20,21]. A study aiming to concurrently optimize both the imaging and the sensing function is still missing.…”

Section: Introductionmentioning

confidence: 99%

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Dielectric Nanohole Array Metasurface For High-Resolution Near-Field Sensing and Imaging

Conteduca

Barth

Pitruzzello

et al. 2020

Preprint

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Dielectric metasurfaces support resonances that are widely explored both for far-field wavefront shaping and for near-field sensing and imaging. Their design explores the interplay between localised Mie resonances and extended Bragg resonances, with a typical trade-off between Q-factor and light localisation; high Q-factors are desirable for refractive index sensing while localisation is desirable for imaging resolution. Here, we show that a dielectric metasurface consisting of a nanohole array in amorphous silicon provides a favourable trade-off between these requirements. We have designed and realised the metasurface to support two optical modes both with sharp Fano resonances that exhibit relatively high Q factors and strong spatial confinement, thereby concurrently optimizing the device for both imaging and biochemical sensing. For the sensing application, we demonstrate a limit of detection (LOD) as low as 1pg/ml for Immunoglobulin G (IgG); for resonant imaging, we demonstrate a spatial resolution below 1µm and clearly resolve individual E.coli bacteria. The combined low LOD and high spatial resolution opens new opportunities for extending cellular studies into the realm of microbiology, e.g. for studying antimicrobial susceptibility.

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Section: Single Bacteria Detection With Resonant Hyperspectral Imagingsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Dielectric Nanohole Array Metasurface For High-Resolution Near-Field Sensing and Imaging

Conteduca

Barth

Pitruzzello

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Although optical clearing of bone has been applied in vivo [58,59] to reduce bone scattering through decalcification and refractive index matching for deeper light penetration, a number of potential problems present obstacles in its application to the inner ear. The main concern is that chemicals used for bone clearing can be toxic to the delicate sensorineural structures within the cochlea.…”

Section: Discussionmentioning

confidence: 99%

Imaging hair cells through laser-ablated cochlear bone

Romito¹,

Pu²,

Stanković³

2019

Biomed. Opt. Express

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We report an innovative technique for the visualization of cells through an overlying scattering medium by combining femtosecond laser bone ablation and two-photon excitation fluorescence (TPEF) microscopy. We demonstrate the technique by imaging hair cells in an intact mouse cochlea ex vivo. Intracochlear imaging is important for the assessment of hearing disorders. However, the small size of the cochlea and its encasement in the densest bone in the body present challenging obstacles, preventing the visualization of the intracochlear microanatomy using standard clinical imaging modalities. The controlled laser ablation reduces the optical scattering of the cochlear bone while the TPEF allows visualization of individual cells behind the bone. We implemented optical coherence tomography (OCT) simultaneously with the laser ablation to enhance the precision of the ablation and prevent inadvertent damage to the cells behind the bone.

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“…These results clearly demonstrate that the metasurface approach can be used for nanoscopic analysis because the refractive index being probed is that of the bacterial membrane. This ability could open up a variety of studies including, for example, cell adhesion and secretion monitoring that have previously only been demonstrated with mammalian cells [79,80], but have never been applied to bacteria. Monitoring the response of bacteria to antibiotic challenge is another promising area of research opened up by these structures, which is discussed in more detail in Section 3.…”

Section: Resonant Metasurfacesmentioning

confidence: 99%

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

2020

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Photonic biosensors are a major topic of research that continues to make exciting advances. Technology has now improved sufficiently for photonics to enter the realm of microbiology and to allow for the detection of individual bacteria. Here, we discuss the different nanophotonic modalities used in this context and highlight the opportunities they offer for studying bacteria. We critically review examples from the recent literature, starting with an overview of photonic devices for the detection of bacteria, followed by a specific analysis of photonic antimicrobial susceptibility tests. We show that the intrinsic advantage of matching the optical probed volume to that of a single, or a few, bacterial cell, affords improved sensitivity while providing additional insight into single-cell properties. We illustrate our argument by comparing traditional culture-based methods, which we term macroscopic, to microscopic free-space optics and nanoscopic guided-wave optics techniques. Particular attention is devoted to this last class by discussing structures such as photonic crystal cavities, plasmonic nanostructures and interferometric configurations. These structures and associated measurement modalities are assessed in terms of limit of detection, response time and ease of implementation. Existing challenges and issues yet to be addressed will be examined and critically discussed.

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Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)

Cited by 20 publications

References 84 publications

Dielectric Nanohole Array Metasurface For High-Resolution Near-Field Sensing and Imaging

Dielectric Nanohole Array Metasurface For High-Resolution Near-Field Sensing and Imaging

Imaging hair cells through laser-ablated cochlear bone

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

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