Quantitative analysis of flavour volatiles detects differences among closely related traditional cultivars of tomato

Ruiz, José L.; Alonso, Arancha; García‐Martínez, Santiago; Valero, Manuel; Blasco, Pilar Santolaria; Ruiz-Beviá, F.

doi:10.1002/jsfa.1879

Cited by 55 publications

(51 citation statements)

References 34 publications

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“…To address whether or not the parthenocarpic phenotype dramatically affects fruit taste, we measured the levels of the most important taste-and flavor-related tomato metabolites (sugars, organic acids, and 16 volatiles; Yilmaz, 2001;Ruiz et al, 2005) in Chs RNAi and wild-type tomatoes. For both wild-type and Chs RNAi fruits, the levels of flavor-related volatile compounds fell well within the variation observed in a collection of 94 commercially available tomato cultivars (Tikunov et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

RNA Interference Silencing of Chalcone Synthase, the First Step in the Flavonoid Biosynthesis Pathway, Leads to Parthenocarpic Tomato Fruits

Schijlen

Vos²,

Martens³

et al. 2007

Plant Physiol.

251

159

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; and Keygene N.V., 6700 AE Wageningen, The Netherlands (A.J.v.T.)Parthenocarpy, the formation of seedless fruits in the absence of functional fertilization, is a desirable trait for several important crop plants, including tomato (Solanum lycopersicum). Seedless fruits can be of great value for consumers, the processing industry, and breeding companies. In this article, we propose a novel strategy to obtain parthenocarpic tomatoes by downregulation of the flavonoid biosynthesis pathway using RNA interference (RNAi)-mediated suppression of chalcone synthase (CHS), the first gene in the flavonoid pathway. In CHS RNAi plants, total flavonoid levels, transcript levels of both Chs1 and Chs2, as well as CHS enzyme activity were reduced by up to a few percent of the corresponding wild-type values. Surprisingly, all strong Chs-silenced tomato lines developed parthenocarpic fruits. Although a relation between flavonoids and parthenocarpic fruit development has never been described, it is well known that flavonoids are essential for pollen development and pollen tube growth and, hence, play an essential role in plant reproduction. The observed parthenocarpic fruit development appeared to be pollination dependent, and Chs RNAi fruits displayed impaired pollen tube growth. Our results lead to novel insight in the mechanisms underlying parthenocarpic fruit development. The potential of this technology for applications in plant breeding and biotechnology will be discussed.

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Section: Discussionmentioning

confidence: 99%

RNA Interference Silencing of Chalcone Synthase, the First Step in the Flavonoid Biosynthesis Pathway, Leads to Parthenocarpic Tomato Fruits

Schijlen

Vos²,

Martens³

et al. 2007

Plant Physiol.

251

159

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“…All substances were known or expected to play a role in tomato taste sensation (Baldwin et al 2000;Tandon et al 2003;Baldwin et al 2004;Krumbein et al 2004;Ruiz et al 2005). …”

Section: Introductionmentioning

confidence: 99%

A correlation network approach to metabolic data analysis for tomato fruits

et al. 2008

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Network analysis of correlations between abundances of metabolites across tomato genotypes can help in unraveling the biological basis of organoleptic variation in tomato. We illustrate how to construct and interpret simple correlations networks using metabolic data collected on a diverse set of tomato genotypes. Various types of correlations are calculated and displayed in the form of networks. Interpretations on the basis of network analyses are compared to interpretations following principal components analysis.

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“…Another significant part of the plant metabolome, comprising the volatile metabolites, is of a particular interest, since they play an important role in fundamental processes such as signaling mechanisms and interorganism interactions (Shulaev et al, 1997;Seskar et al, 1998;Ozawa et al, 2000;Arimura et al, 2002;Liechti and Farmer, 2002;Dicke et al, 2003;Dudareva et al, 2004;Engelberth et al, 2004;Ryu et al, 2004). In addition, these components are also of great agronomic importance as volatile metabolites are major determinants of food and flower quality in terms of flavor and fragrance (Buttery and Ling, 1993;Baldwin et al, 2000Baldwin et al, , 2004Yilmaz et al, 2001;Tandon et al, 2003;Krumbein et al, 2004;Simkin et al, 2004;Ruiz et al, 2005).…”

mentioning

confidence: 99%

A Novel Approach for Nontargeted Data Analysis for Metabolomics. Large-Scale Profiling of Tomato Fruit Volatiles

Tikunov

Lommen²,

Vos³

et al. 2005

Plant Physiology

453

410

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To take full advantage of the power of functional genomics technologies and in particular those for metabolomics, both the analytical approach and the strategy chosen for data analysis need to be as unbiased and comprehensive as possible. Existing approaches to analyze metabolomic data still do not allow a fast and unbiased comparative analysis of the metabolic composition of the hundreds of genotypes that are often the target of modern investigations. We have now developed a novel strategy to analyze such metabolomic data. This approach consists of (1) full mass spectral alignment of gas chromatography (GC)-mass spectrometry (MS) metabolic profiles using the MetAlign software package, (2) followed by multivariate comparative analysis of metabolic phenotypes at the level of individual molecular fragments, and (3) multivariate mass spectral reconstruction, a method allowing metabolite discrimination, recognition, and identification. This approach has allowed a fast and unbiased comparative multivariate analysis of the volatile metabolite composition of ripe fruits of 94 tomato (Lycopersicon esculentum Mill.) genotypes, based on intensity patterns of .20,000 individual molecular fragments throughout 198 GC-MS datasets. Variation in metabolite composition, both between-and within-fruit types, was found and the discriminative metabolites were revealed. In the entire genotype set, a total of 322 different compounds could be distinguished using multivariate mass spectral reconstruction. A hierarchical cluster analysis of these metabolites resulted in clustering of structurally related metabolites derived from the same biochemical precursors. The approach chosen will further enhance the comprehensiveness of GC-MS-based metabolomics approaches and will therefore prove a useful addition to nontargeted functional genomics research.

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Quantitative analysis of flavour volatiles detects differences among closely related traditional cultivars of tomato

Cited by 55 publications

References 34 publications

RNA Interference Silencing of Chalcone Synthase, the First Step in the Flavonoid Biosynthesis Pathway, Leads to Parthenocarpic Tomato Fruits

RNA Interference Silencing of Chalcone Synthase, the First Step in the Flavonoid Biosynthesis Pathway, Leads to Parthenocarpic Tomato Fruits

A correlation network approach to metabolic data analysis for tomato fruits

A Novel Approach for Nontargeted Data Analysis for Metabolomics. Large-Scale Profiling of Tomato Fruit Volatiles

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