Quantitative analysis of flavonoids and phenolic acids in propolis by two-dimensional thin layer chromatography

Medić-Šarič, Marica; Jasprica, Ivona; Mornar, Ana; Smolčić-Bubalo, Asja; Golja, Petra

doi:10.1556/jpc.17.2004.6.12

Cited by 37 publications

(19 citation statements)

References 14 publications

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“…The compounds reported by different authors are caffeic acid; p-coumaric acid; 3,4-dimethoxycinnamic acid; quercetin; pinobanksin 5-methyl ether; apigenin; kaempferol; pinobanksin; cinnamylideneacetic acid; chrysin; pinocembrin; galangin; pinobanksin 3-acetate; phenethyl caffeate; cinnamyl caffeate; tectochrysin; artepillin C (Marcucci and Bankova, 1999;Medic-Saric et al, 2004;Kumazawa et al, 2004).…”

Section: Total Phenolic Contentsmentioning

confidence: 99%

Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal

Moreira

Dias

Pereira

et al. 2008

Food and Chemical Toxicology

235

167

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a b s t r a c tPollen analysis, total phenols content and antioxidant activity were studied for the first time in Portuguese propolis samples from Bornes and Fundão regions. Total phenols content was determined by colorimetric assay and their amount was of 329 mg/g of GAE in Bornes sample and 151 mg/g of GAE in Fundão propolis. The antioxidant capacity of propolis extracts was assessed through the scavenging effects on DPPH (2,2-diphenyl-1-picrylhydrazyl) and reducing power of iron (III)/ ferricyanide complex assays. A concentration-dependent antioxidative capacity was verified in DPPH and reducing power assays. Low values of EC 50 on DPPH scavenging assay were obtained for Bornes and Fundão propolis (of 6.22 lg/mL and 52.00 lg/mL, respectively). For reducing power the values were 9.00 lg/mL, for Bornes propolis, and 55.00 lg/mL, for Fundão propolis. The high activity of propolis from Bornes could be related with their different pollen composition. The results obtained indicate that Portuguese propolis is an important source of total phenols showing antioxidant properties that could be beneficial for human health.

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Section: Total Phenolic Contentsmentioning

confidence: 99%

Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal

Moreira

Dias

Pereira

et al. 2008

Food and Chemical Toxicology

235

167

View full text Add to dashboard Cite

show abstract

“…Medic-Saric et al [133] used two-dimensional TLC with densitometric evaluation with n-hexane/ethyl acetate/glacial acetic acid (31:14:5, nu/nu) (System A) and chloroform/methanol/formic acid (44:3.5:2.5) (System B) as mobile phases.…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

Advances in the analysis of phenolic compounds in products derived from bees

Gómez-Caravaca

Gómez-Romero

Arráez‐Román

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

355

224

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“…A large number of analytical methods have been used for the analysis of phenolic compounds in propolis, including spectrophotometry [17], thin-layer chromatography [18], gas chromatography-mass spectrometry (GC-MS) [19], highperformance liquid chromatography (HPLC) [20], liquid chromatography-mass spectrometry (LC-MS) [21][22][23], electrospray ionization-mass spectrometry (ESI-MS) and electrospray ionization-tandem mass spectrometry (ESI-MS n ) [24], capillary electrophoresis [25], and nuclear magnetic resonance (NMR) [26]. Among these methods, MS with ESI is one of the most-used techniques in the research of natural products, as it is able to analyze complex mixtures because of its high selectivity.…”

Section: Introductionmentioning

confidence: 99%

Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds

et al. 2009

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In this study, an ethanolic extract from Portuguese propolis was prepared, fractionated by highperformance liquid chromatography, and the identification of the phenolic compounds was done by electrospray mass spectrometry in the negative mode. This technical approach allowed the identification of 37 phenolic compounds, which included not only the typical phenolic acids and flavonoids found in propolis from temperate zones but also several compounds in which its occurrence have never been referred to in the literature. Four of the novel phenolic compounds were methylated and/or esterified or hydroxylated derivatives of common poplar flavonoids, although six peculiar derivatives of pinocembrin/pinobanksin, containing a phenylpropanoic acid derivative moiety in their structure, were also identified. Furthermore, the Portuguese propolis sample was shown to contain a p-coumaric ester derivative dimer.

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Quantitative analysis of flavonoids and phenolic acids in propolis by two-dimensional thin layer chromatography

Cited by 37 publications

References 14 publications

Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal

Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal

Advances in the analysis of phenolic compounds in products derived from bees

Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds

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