Quantitative analysis of DHEA and androsterone in female urine: investigating the effects of menstrual cycle, oral contraception and training on exercise-induced changes in young women

Bayle, Maxime; Enéa, Carina; Goetinck, Patrick; Lafay, Florent; Dugué, Benoît; Flament-Waton, Marie-Magdeleine; Grenier-Loustalot, M. F.

doi:10.1007/s00216-008-2549-z

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Hormonal contraceptives reduce cortisol release in response to stress (Simunkova et al, 2008;Roche et al, 2013) and lower DHEA(S) excretion (Greco et al, 2007;Bayle et al, 2009), and therefore two patients and four controls were excluded from the analysis. One patient who showed an extreme outlier value for the cortisol/DHEAS ratio (34.5, a level comparable to that observed in females using contraceptives) was also excluded from the analysis.…”

Section: Participantsmentioning

confidence: 99%

The relationship between stress, HPA axis functioning and brain structure in first episode psychosis over the first 12 weeks of treatment

Reniers

Garner

Phassouliotis

et al. 2015

Psychiatry Research: Neuroimaging

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a b s t r a c tStress and abnormal hypothalamic-pituitary-adrenal axis functioning have been implicated in the early phase of psychosis and may partly explain reported changes in brain structure. This study used magnetic resonance imaging to investigate whether biological measures of stress were related to brain structure at baseline and to structural changes over the first 12 weeks of treatment in first episode patients (n ¼22) compared with matched healthy controls (n ¼ 22). At baseline, no significant group differences in biological measures of stress, cortical thickness or hippocampal volume were observed, but a significantly stronger relationship between baseline levels of cortisol and smaller white matter volumes of the cuneus and anterior cingulate was found in patients compared with controls. Over the first 12 weeks of treatment, patients showed a significant reduction in thickness of the posterior cingulate compared with controls. Patients also showed a significant positive relationship between baseline cortisol and increases in hippocampal volume over time, suggestive of brain swelling in association with psychotic exacerbation, while no such relationship was observed in controls. The current findings provide some support for the involvement of stress mechanisms in the pathophysiology of early psychosis, but the changes are subtle and warrant further investigation.

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Section: Participantsmentioning

confidence: 99%

The relationship between stress, HPA axis functioning and brain structure in first episode psychosis over the first 12 weeks of treatment

Reniers

Garner

Phassouliotis

et al. 2015

Psychiatry Research: Neuroimaging

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“…It was possible with our quantitative method to get an idea of the concentration levels of DHEA and several of its metabolites after intake of DHEA, which was unknown in calves until now. Naturally, DHEA is mainly present in blood and urine of older animals in its sulphated form and to a much lesser extent in its free form or as a glucuronide conjugate [11]. The sample clean-up was set up such that each form, i.e.…”

Section: Introductionmentioning

confidence: 99%

Investigation of urinary steroid metabolites in calf urine after oral and intramuscular administration of DHEA

Becue

Poucke

Rijk³

et al. 2009

Anal Bioanal Chem

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DHEA (3β-hydroxy-androst-5-en-17-one) is a natural steroid prohormone. Despite a lack of information on the effect, DHEA and other prohormones are frequently used as a food supplement by body-builders. DHEA is suspected for growth promoting abuse in cattle as well. Considering the latter, urine samples from a previous exposure study in which calves were exposed to 1 g DHEA per day during 7 days, were used. The calves were divided in three groups: one orally treated, one intramuscularly injected and a control group. The effect of this treatment on the urinary profile of several precursors and metabolites of DHEA was investigated. Urine samples were collected several days before and during the 7 days of administration and were submitted to a clean-up procedure consisting of a separation of the different conjugates (free, glucuronidated and sulphated forms) of each compound on a SAX column (Varian). An LC-MS/MS method was developed for the detection and quantification of several metabolites of the pathway of DHEA including 17α-and 17β-testosterone, 4-androstenedione, 5-androstenediol, pregnenolone and hydroxypregnenolone. Elevated levels of DHEA, 5-androstenediol and 17α-testosterone were observed in the free and sulphated fraction of the urine of the treated calves, thus indicating that the administered DHEA is metabolized mainly by the ∆ 5 -pathway with 5-androstenediol as the intermediate.Sulphoconjugates of DHEA and its metabolites were found to constitute the largest proportion of the urinary metabolites. The free form was also present, but in a lesser extent than the sulphated form, while glucuronides were negligible.

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Strength training effects on urinary steroid profile across the menstrual cycle in healthy women

et al. 2012

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Some studies suggest that performing strength training may cause alterations on the hypothalamic pituitary axis, resulting in steroid hormone variations. Intense training has been associated to slow the concentrations of estrogens and progesterone in women. The main purpose of this study was to evaluate the effects of strength training on the urinary steroid concentrations across the menstrual cycle phases. Twenty healthy women, regularly menstruating and not using pharmacologic contraceptives, performed a strength training during 8 weeks. Participants worked out 3 sets × 10 repetitions, with 2 min recovery time between sets, at 70-75 % of one maximum strength repetition. Urine samples were taken in three different phases of the menstrual cycle (menstrual, follicular and luteal) and they were collected both before and after training. Testosterone, DHEA, cortisol, cortisone, estradiol and progesterone concentrations were determined by gas chromatography-mass spectrometry. The results showed a significant decline after training in the urinary excretion of estradiol, during the menstrual and follicular phase, and progesterone, during the menstrual and luteal phase. No significant difference was observed for other steroid hormones. These data demonstrated that strength training can play an important role in the estrogen and progesterone metabolism in women, decreasing their levels across the menstrual cycle.

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Quantitative analysis of DHEA and androsterone in female urine: investigating the effects of menstrual cycle, oral contraception and training on exercise-induced changes in young women

Cited by 5 publications

References 39 publications

The relationship between stress, HPA axis functioning and brain structure in first episode psychosis over the first 12 weeks of treatment

The relationship between stress, HPA axis functioning and brain structure in first episode psychosis over the first 12 weeks of treatment

Investigation of urinary steroid metabolites in calf urine after oral and intramuscular administration of DHEA

Strength training effects on urinary steroid profile across the menstrual cycle in healthy women

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