Quantitative analysis of clenbuterol in meat products using liquid chromatography–electrospray ionisation tandem mass spectrometry

Validation and application of a screening method for β2‐agonists, anti‐estrogenic substances and mesocarb in human urine using liquid chromatography/tandem mass spectrometry

“…Detection of these drugs in routine analysis from biological matrices has been accomplished mainly by gas chromatography/mass spectrometry (GC/ MS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) has been extensively used since electrospray ionization (ESI) was adapted. [5][6][7][8][9][10][11][12] The LC/MS/MS detection method has the advantage of simple sample preparation and efficient ionization for hydrophilic compounds. Until now several LC/MS/MS methods have been suggested for the analysis of b-agonists.…”

“…Multi-b-agonists were quantified in bovine urine using LC/MS by Cai and Henion, 5 Lau et al 9 and Dickson et al 10 These methods include the detection of clenbuterol and salbutamol among b2-agonists. A detection method for clenbuterol was developed from meat products, 6 equine plasma and urine. 7 A detection method for salmeterol in equine urine was also developed by Eenoo et al 8 Thevis et al have developed screening and confirmation methods for b2-agonists using tandem mass spectrometry.…”

Screening and Confirmatory Determination of Clenbuterol Residues in Bovine Meat Marketed in the Northwest of Mexico Monitoreo Y Confirmación De La Presencia De Residuos De Clenbuterol en Carne De Bovino Comercializada en El Noroeste De México

“…The ELISA technique showed very high correlation between clenbuterol concentration (ng/kg) and maximum % absorbance. The calibration curve constructed for the ELISA determinations (y = -18.396 Ln(x) + 184.68) followed a relationship with a highly significant (P < 0.01) coefficient of multiple determination (R 2 = 0.999), where y was maximum absorbance (%) and x was clenbuterol from meat products was much lower (63%) at a fortification level of 0.4 µg/kg and determined by LC-MS-MS (Guy et al, 1999). Similarly, clenbuterol recoveries of 44-75 % from urine samples spiked with 0.2 to 1.5 µg/kg were detected by using ELISA (Sawaya et al, 2000).…”

Sulfonated polystyrene magnetic nanobeads coupled with immunochromatographic strip for clenbuterol determination in pork muscle

“…Currently, a few commercial solid-phase extraction cartridges with different surface functional groups have been widely used in CLE sample preparation. Examples of these cartridges include reversed-phase [13], strong cationexchange [14], and mixed-mode cation-exchange [15] cartridges. Mixed-mode cartridges show higher sensitivity, better loading capacity, and better efficiencies than single-mode stationaryphase cartridges in CLE sample pretreatment because of the presence of interactions that contribute to analyte retention [16].…”