Quantitative analysis of classical and new putative periodontal pathogens in subgingival biofilm: a case–control study

Al-Hebshi, Nezar N.; Al-Alimi, Abdulrahman; Taiyeb-Ali, Tara B.; Jaafar, Nasruddin

doi:10.1111/jre.12210

Cited by 30 publications

(26 citation statements)

References 45 publications

(81 reference statements)

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“…Da Silva-Boghossian et al [11] observed a high prevalence of red complex bacteria in subjects with periodontal diseases, and Abiko et al [1] identified Tannerella forsythia and P. gingivalis as the predominant pathogens in the course of periodontitis in humans. In contrast, Al-Hebsi et al [4], in their study of bacterial microflora in humans with chronic periodontitis, reported a low correlation between P. gingivalis and periodontal disease when compared to the correlations with other evaluated bacteria; those results contradict the results in this study.…”

Section: Discussioncontrasting

confidence: 99%

Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease

Gołyńska

Polkowska

Bartoszcze-Tomaszewska³

et al. 2017

J Vet Sci

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Dogs commonly serve as a model for various human conditions, including periodontal diseases. The aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in dogs and humans by using rapid real-time polymerase chain reaction (RT-PCR)-based tests and to compare the results obtained in each species. Bacterial microflora evaluations, both quantitative and qualitative, were performed by applying ready-made tests on twelve dogs and twelve humans. Five samples were collected from each subject's deepest gingival pockets and joined to form a collective sample. The results of the study revealed interspecies similarities in the prevalences of Porphyromonas (P.) gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum. Red complex bacteria comprised the largest portion of the studied bacterial complexes in all study groups, with P. gingivalis being the most commonly isolated bacterium. The results show similarities in the prevalence of bacterial microflora in dogs and humans. Microbiological analysis of gingival pockets by using rapid real-time PCR-based tests in clinical practice, both veterinary and human, can facilitate the choice of appropriate pharmacological treatment and can provide a basis for subsequent verification of the treatment's effectiveness.

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Section: Discussioncontrasting

confidence: 99%

Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease

Gołyńska

Polkowska

Bartoszcze-Tomaszewska³

et al. 2017

J Vet Sci

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“…It would be of interest to investigate the plaque with high‐throughput techniques, whereas DNA–DNA checkerboard hybridization technique has a rather crude accuracy. Keystone pathogens other than P. gingivalis may be determined for host response in smokers and Parvimonas mirca is considered a keystone pathogen associated with deep pockets in smokers . Nevertheless, the majority of immune responses to periopathogens are known to occur locally within the periodontal tissues, in gingival crevicular fluid and between cells conjugated to another, rather than to systemic responses .…”

Section: Discussionmentioning

confidence: 99%

The effect of smoking on inflammatory and bone remodeling markers in gingival crevicular fluid and subgingival microbiota following periodontal therapy

Bunæs

Mustafa

Mohamed

et al. 2017

J of Periodontal Research

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Except for an upregulation of interleukin-8, smokers exhibited reduced gingival crevicular fluid levels of several inflammatory markers at baseline and following active and supportive periodontal therapy. Only inflammatory responses in non-smokers adapted to periodontal therapy. Apparently, there seems to be an immunosuppressant effect of smoking regulating the local inflammatory response and bone remodeling markers captured in gingival crevicular fluid following periodontal therapy.

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“…Recently, in a case control study, P. micra was found to be the only microbial predictor of periodontal parameters. P. micra is regarded as an important periodontal pathogen warranting more attention [57]. …”

Section: Discussionmentioning

confidence: 99%

Accuracy of commercial kits and published primer pairs for the detection of periodontopathogens

et al. 2016

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ObjectivesDespite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. The aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens.Materials and methodsFourteen bacterial mock communities, consisting of 16 randomly assembled bacterial strains, were used as reference standard for testing kits and primers. Extracted DNA from mock communities was analyzed by PCR in-house with specific primers and forwarded for analysis to the manufacturer’s laboratory of each of the following kits: ParoCheck®Kit 20, micro-IDent®plus11, and Carpegen® Perio Diagnostik.ResultsThe kits accurately detected Fusobacterium nucleatum, Prevotella intermedia/Prevotella nigrescens, Parvimonas micra, Aggregatibacter actinomycetemcomitans, Campylobacter rectus/showae, Streptococcus mitis, Streptococcus mutans, and Veillonella parvula. The in-house primers for F.nucleatum were highly specific to subtypes of the respective periopathogen. Other primers repeatedly detected oral pathogens not present in the mock communities, indicating reduced specificity.ConclusionsThe commercial kits used in this study are reliable tools to support periodontal diagnostics. Whereas the detection profile of the kits is fixed at a general specificity level, the design of primers can be adjusted to differentiate between highly specific strains. In-house primers are more error-prone. Bacterial mock communities can be established as a reference standard for any similar testing.Clinical relevanceThe tested kits render good results with selected bacterial species. Primers appear to be less useful for routine clinical diagnostics and of limited applicability in research. Basic information about the periodontopathogens identified in this study supports clinical decision-making.

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Quantitative analysis of classical and new putative periodontal pathogens in subgingival biofilm: a case–control study

Abstract: Oral Synergistetes are presented here as new members of the red complex, with relative importance to periodontitis exceeding that of the classical members. P. micra is shown as an important periodontal pathogen warranting more attention.

Cited by 30 publications

References 45 publications

Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease

Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease

The effect of smoking on inflammatory and bone remodeling markers in gingival crevicular fluid and subgingival microbiota following periodontal therapy

Accuracy of commercial kits and published primer pairs for the detection of periodontopathogens

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