Quantitative analysis of cellular proteome alterations of Pseudomonas putida to naphthalene-induced stress

Li, Shanshan; Hu, Xiao; Zhao, Hong; Li, Ya-Xiao; Zhang, Li; Gong, Lijun; Guo, Jin; Zhao, Huabing

doi:10.1007/s10529-015-1828-y

Cited by 15 publications

(9 citation statements)

References 27 publications

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“…Therefore, its increased abundance during P. putida KT2440 growth was important for aggregated proteins to renature after exposure to unfavorable conditions. A similar situation was observed during cellular proteome alterations of Pseudomonas putida to naphthalene-induced stress [30]. Furthermore, the upregulation of quinoprotein ethanol dehydrogenase protein could be related to the protection against the abnormal formation of reactive oxygen species (ROS).…”

Section: Stress Responsesupporting

confidence: 54%

Proteomic Response of Pseudomonas putida KT2440 to Dual Carbon-Phosphorus Limitation during mcl-PHAs Synthesis

Możejko-Ciesielska

Serafim

2019

Biomolecules

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Pseudomonas putida KT2440, one of the best characterized pseudomonads, is a metabolically versatile producer of medium-chain-length polyhydroxyalkanoates (mcl-PHAs) that serves as a model bacterium for molecular studies. The synthesis of mcl-PHAs is of great interest due to their commercial potential. Carbon and phosphorus are the essential nutrients for growth and their limitation can trigger mcl-PHAs’ production in microorganisms. However, the specific molecular mechanisms that drive this synthesis in Pseudomonas species under unfavorable growth conditions remain poorly understood. Therefore, the proteomic responses of Pseudomonas putida KT2440 to the limited carbon and phosphorus levels in the different growth phases during mcl-PHAs synthesis were investigated. The data indicated that biopolymers’ production was associated with the cell growth of P. putida KT2440 under carbon- and phosphorus-limiting conditions. The protein expression pattern changed during mcl-PHAs synthesis and accumulation, and during the different physiological states of the microorganism. The data suggested that the majority of metabolic activities ceased under carbon and phosphorus limitation. The abundance of polyhydroxyalkanoate granule-associated protein (PhaF) involved in PHA synthesis increased significantly at 24 and 48 h of the cultivations. The activation of proteins belonging to the phosphate regulon was also detected. Moreover, these results indicated changes in the protein profiles related to amino acids metabolism, replication, transcription, translation, stress response mechanisms, transport or signal transduction. The presented data allowed the investigation of time-course proteome alterations in response to carbon and phosphorus limitation, and PHAs synthesis. This study provided information about proteins that can be potential targets in improving the efficiency of mcl-PHAs synthesis.

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Section: Stress Responsesupporting

confidence: 54%

Proteomic Response of Pseudomonas putida KT2440 to Dual Carbon-Phosphorus Limitation during mcl-PHAs Synthesis

Możejko-Ciesielska

Serafim

2019

Biomolecules

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“…Therefore, in order to survive from the three treatments, E. coli cells might synthesize more molecular chaperones to regulate metabolism, which resulted in reduction of the most amino acids. Another reason for more down-regulation of amino acids might due to the ATP deficiency in response to heat shock from the three treatments, where synthesis of amino acids was required ATP as the energy source (Li et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…Energy is required in basic metabolism, which includes syntheses of proteins, DNA, and RNA (Li et al, 2015). There were 8 metabolites involved in energy metabolism, 7 of them were presented after LVLT, but only 3 and 4 metabolites were presented after HVST and WB.…”

Section: Resultsmentioning

confidence: 99%

New Insights Into the Response of Metabolome of Escherichia coli O157:H7 to Ohmic Heating

Tian

Yao

et al. 2018

Front. Microbiol.

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The objective of this study was to investigate the effects of ohmic heating and water bath heating (WB) on the metabolome of Escherichia coli O157:H7 cells at the same inactivation levels. Compared to low voltage long time ohmic heating (5 V/cm, 8.50 min, LVLT) and WB (5.50 min), the high voltage short time ohmic heating (10 V/cm, 1.75 min, HVST) had much shorter heating time. Compared to the samples of control (CT), there were a total of 213 differential metabolites identified, among them, 73, 78, and 62 were presented in HVST, LVLT, and WB samples, revealing a stronger metabolomic response of E. coli cells to HVST and LVLT than WB. KEGG enrichment analysis indicated that the significantly enriched pathways were biosynthesis and metabolism of amino acids (alanine, arginine, aspartate, and glutamate, etc.), followed by aminoacyl-tRNA biosynthesis among the three treatments. This is the first metabolomic study of E. coli cells in response to ohmic heating and presents an important step toward understanding the mechanism of ohmic heating on microbial inactivation, and can serve as a theoretical basis for better application of ohmic heating in food products.

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“…The proteins in the activated sludge samples were extracted using a modified method as follows: 22 0.2 g of activated sludge was resuspended in 10 mL of Tris–SDS/Tris–saturated phenol lysate. Following sonication, the supernatant phase was transferred into 30 mL of a pre-cooled ammonium acetate/methanol solution (0.1 mol L −1 ) to precipitate the proteins.…”

Section: Methodsmentioning

confidence: 99%

Microbial diversity and metaproteomic analysis of activated sludge responses to naphthalene and anthracene exposure

Shi

et al. 2019

RSC Adv.

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The activated sludge process can effectively remove polycyclic aromatic hydrocarbons (PAHs) from wastewater via biodegradation. However, the degradable microorganisms and functional enzymes involved in this process remain unclear. In this study, we successfully employed a laboratory-scale sequential batch reactor to investigate variations in microbial community and protein expression in response to the addition of different PAHs and process time. The analysis of bacterial community structure by 454 pyrosequencing of the 16S rRNA gene indicated that bacteria from Burkholderiales order were dominant in PAHs treated sludge. Mass spectrometry performed with 2D protein profiles of all sludge samples demonstrated that most proteins exhibiting differential expression profiles during the process were derived from Burkholderiales populations; these proteins are involved in DNA replication, fatty acid and glucose metabolism, stress response, protein synthesis, and aromatic hydrocarbon metabolism. Nevertheless, the protein expression profiles indicated that naphthalene, but not anthracene, can induce the expression of PAH-degrading proteins and accelerate its elimination from sludge. Though only naphthalene and anthracene were added into our experimental groups, the differentially expressed enzymes involved in other PAHs (especially biphenyl) metabolism were also detected. This study provides apparent evidence linking the metabolic activities of Burkholderiales populations with the degradation of PAHs in activated sludge processes. Overall, our findings highlighted the successful application of metaproteomics integrated with microbial diversity analysis by high-throughput sequencing technique on the analysis of environmental samples, which could provide a convenience to monitor the changes in proteins expression profiles and their correlation with microbial diversity. View Article Onlinea NAP eff : average effluent naphthalene concentration; ANT eff : average effluent anthracene concentration; NAP sludge : naphthalene in sludge; ANT sludge : anthracene in sludge; E NAP : naphthalene removal efficiency; E ANT : anthracene removal efficiency; E-BIO NAP : biodegradation efficiency of naphthalene; E-BIO ANT : biodegradation efficiency of anthracene. b n ¼ 3, mean AE SD.22844 | RSC Adv., 2019,9,[22841][22842][22843][22844][22845][22846][22847][22848][22849][22850][22851][22852] This journal is View Article Online 356 in naphthalene 1,2-dioxygenase (N1,2-DO, EC 1.14.12.12) of Pseudomonas putida G7. Data showed that there was an efficient electron transfer between the BDO reductase and the N1,2-DO ferredoxin, the BDO ferredoxin could resubstitute N1,2-DO ferredoxin in part (22%), which indicated that BDO might participate in naphthalene degradation. 41 Furthermore, Yu et al. Fig. 3 2D PAGE profiles of proteins extracted from the experimental groups. Potential protein molecular mass ranges are indicated on the left and isoelectric point ranges are provided on the top of the gel images.22848 | RSC Adv., 2019,9,[22841][22842][22843][...

show abstract

Quantitative analysis of cellular proteome alterations of Pseudomonas putida to naphthalene-induced stress

Abstract: Our findings offer insights into the cellular response of P. putida to high naphthalene concentrations at the protein level.

Cited by 15 publications

References 27 publications

Proteomic Response of Pseudomonas putida KT2440 to Dual Carbon-Phosphorus Limitation during mcl-PHAs Synthesis

Proteomic Response of Pseudomonas putida KT2440 to Dual Carbon-Phosphorus Limitation during mcl-PHAs Synthesis

New Insights Into the Response of Metabolome of Escherichia coli O157:H7 to Ohmic Heating

Microbial diversity and metaproteomic analysis of activated sludge responses to naphthalene and anthracene exposure

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