Quantitative analysis of brain perfusion SPECT in Alzheimer's disease using a fully automated regional cerebral blood flow quantification software, 3DSRT

Kobayashi, Seiju; Tateno, Masaru; Utsumi, Kumiko; Takahashi, Akira; Saitoh, Masaki; Morii, Hidetoshi; Fujii, Kazuki; Teraoka, Masatoshi

doi:10.1016/j.jns.2007.07.015

Cited by 42 publications

(38 citation statements)

References 26 publications

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“…Moreover, curcumin has high affinity to Aβ 42 , oligomers, and fibrils, which are tightly linked to AD (62)(63)(64)(65)(66)(67)(68). Curcumin intensely stains the central dense amyloid core in Aβ fibrils and plaques, as was also previously demonstrated in the brain of murine models of AD (101).…”

Section: Discussionsupporting

confidence: 70%

“…To visualize retinal Aβ pathology in vivo, we had previously developed a noninvasive retinal amyloid imaging method for rodent ADtg models, using curcumin as a contrast agent (40,51). Curcumin is a natural and safe phytopolylphenol fluorochrome that binds to Aβ fibrils and oligomers with high affinity and specificity (62)(63)(64)(65)(66)(67)(68). This approach enabled noninvasive detection and monitoring of individual retinal Aβ deposits in live ADtg mice (40,51).…”

Section: Introductionmentioning

confidence: 99%

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Retinal amyloid pathology and proof-of-concept imaging trial in Alzheimer’s disease

et al. 2017

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BACKGROUND. Noninvasive detection of Alzheimer's disease (AD) with high specificity and sensitivity can greatly facilitate identification of at-risk populations for earlier, more effective intervention. AD patients exhibit a myriad of retinal pathologies, including hallmark amyloid β-protein (Aβ) deposits. METHODS.Burden, distribution, cellular layer, and structure of retinal Aβ plaques were analyzed in flat mounts and cross sections of definite AD patients and controls (n = 37). In a proof-of-concept retinal imaging trial (n = 16), amyloid probe curcumin formulation was determined and protocol was established for retinal amyloid imaging in live patients. RESULTS.Histological examination uncovered classical and neuritic-like Aβ deposits with increased retinal Aβ 42 plaques (4.7-fold; P = 0.0063) and neuronal loss (P = 0.0023) in AD patients versus matched controls. Retinal Aβ plaque mirrored brain pathology, especially in the primary visual cortex (P = 0.0097 to P = 0.0018; Pearson's r = 0.84-0.91). Retinal deposits often associated with blood vessels and occurred in hot spot peripheral regions of the superior quadrant and innermost retinal layers. Transmission electron microscopy revealed retinal Aβ assembled into protofibrils and fibrils. Moreover, the ability to image retinal amyloid deposits with solid-lipid curcumin and a modified scanning laser ophthalmoscope was demonstrated in live patients. A fully automated calculation of the retinal amyloid index (RAI), a quantitative measure of increased curcumin fluorescence, was constructed. Analysis of RAI scores showed a 2.1-fold increase in AD patients versus controls (P = 0.0031). CONCLUSION.The geometric distribution and increased burden of retinal amyloid pathology in AD, together with the feasibility to noninvasively detect discrete retinal amyloid deposits in living patients, may lead to a practical approach for large-scale AD diagnosis and monitoring.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Retinal amyloid pathology and proof-of-concept imaging trial in Alzheimer’s disease

et al. 2017

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“…As long as we employ manual settings for the ROI, the reproducibility and objectivity of the results will not be ideal, because the ROIs must be equally placed on SPECT referential slices for every subject. 3DSRT, not an abbreviation but the name of the program, is an automated rCBF quantification program which compensates for the weaknesses of manual quantitative methods by enabling us to set universal ROIs with the minimum of subjectivity [4,12] . The quantified values can be compared among subjects or with the database provided by other researchers.…”

Section: Discussionmentioning

confidence: 99%

“…Quantitative flow-mapping images were obtained from the qualitative perfusion images by using Patlak plot graphical analysis and Lassen's correction [10] . Regional CBF (rCBF) was quantified using an automated brain perfusion SPECT analyzing program, 3DSRT [4,11,12] . This program includes anatomic standardization of images employing statistical parametric mapping (SPM) [13] only for that purpose, rCBF quantification using a three-dimensional stereotactic region of interest (ROI) template, calculation of CBF, and display of the results.…”

Section: Subjectsmentioning

confidence: 99%

Comparison of the Usefulness of Brain Perfusion SPECT and MIBG Myocardial Scintigraphy for the Diagnosis of Dementia with Lewy Bodies

Tateno

Kobayashi²,

Shirasaka³

et al. 2008

Dement Geriatr Cogn Disord

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Background: Supportive features in the diagnostic criteria for dementia with Lewy bodies (DLB) include occipital hypoperfusion and decreased cardiac uptake of ¹²³I-metaiodobenzylguanidine (MIBG). In this study, we performed both brain perfusion single photon emission computed tomography (SPECT) and MIBG myocardial scintigraphy in the same subjects and evaluated their sensitivity to detect the characteristic features of DLB. Methods: Twenty-five patients with probable DLB (76.8 ± 5.1 years old, 10 male) underwent ^99mTc-ethylcysteinate dimer brain perfusion SPECT and ¹²³I-MIBG myocardial scintigraphy. The results of SPECT were analyzed using a qualitative analysis program, easy Z score imaging system (eZIS), and an automated quantitative analysis program, 3DSRT. Results: Qualitative analysis using eZIS demonstrated occipital hypoperfusion in 17 subjects (68%). The quantified mean blood perfusion in the occipital segment on the 3DSRT template was 40.7 ± 5.03 ml/100 g/min (right) and 40.5 ± 5.38 ml/100 g/min (left), and in 19 DLB patients these values were below the normal limit. Twenty-four of 25 subjects (96%) had decreased cardiac MIBG uptake in the delayed image. Conclusion: MIBG myocardial scintigraphy was superior to brain perfusion SPECT in detecting a characteristic feature of DLB. Our results suggest that combining SPECT and MIBG scintigraphy could increase the accuracy of clinical diagnosis of DLB.

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“…Mean uptake values are averaged within these regions and compared within or between subjects [12]. Quantitative methods, such as the automated multi-ROI programme 3DSRT [13,14], statistical parametric mapping [15][16][17], discriminant function analysis [18][19][20], three-dimensional stereotactic surface projection [21] and neural network analysis [22][23][24][25][26] have been added to the research tools available in PET and SPECT. Adding structural information from MRI or CT improves clinical and research interpretations of functional scans [19,[27][28][29][30].…”

Section: Image Analysis Methodsmentioning

confidence: 99%

Is there Still a Place for Perfusion SPECT in the Diagnosis of Dementia?

Ebmeier

2010

TONMEDJ

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Abstract:Although research interest within functional imaging has moved towards applications of MRI, such as BOLD and perfusion imaging, there is a wealth of clinical experience in emission tomographic imaging techniques that make the use of these modalities relevant for the decades to come. This review touches upon the technical and practical issues that distinguish SPECT from PET, describes perfusion and metabolic changes observed in the dementias, compares the clinical utility of the two techniques, and reports data on clinical sensitivity and specificity, as well as diagnostic head-to head comparisons in dementia, and specifically Alzheimer's disease. While few centres have a genuine choice between PET and SPECT, either appears to be good enough to help with the differential diagnosis of dementia in difficult cases.

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Quantitative analysis of brain perfusion SPECT in Alzheimer's disease using a fully automated regional cerebral blood flow quantification software, 3DSRT

Cited by 42 publications

References 26 publications

Retinal amyloid pathology and proof-of-concept imaging trial in Alzheimer’s disease

Retinal amyloid pathology and proof-of-concept imaging trial in Alzheimer’s disease

Comparison of the Usefulness of Brain Perfusion SPECT and MIBG Myocardial Scintigraphy for the Diagnosis of Dementia with Lewy Bodies

Is there Still a Place for Perfusion SPECT in the Diagnosis of Dementia?

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