Quantitative analysis of bone marrow CD34 cells in aplastic anemia and hypoplastic myelodysplastic syndromes

Quantification of marrow CD34+ cells: an important tool for distinguishing between hypoplastic myelodysplastic syndromes and aplastic anemia

“…The paper by Matsui et al 1 is, however, highly inaccurate in the way it quotes our previous study. The authors seem to imply that we did not observe significant differences between the two groups and they stress the 'overlapping' results obtained by several groups and by us (see authors' reference 10).…”

“…Reply to 'Quantification of marrow CD34 þ cells: an important tool for distinguishing between hypoplastic myelodysplastic syndromes and aplastic anemia' by A Orazi The intent of our study 1,2 was not to displace previous reports (including the one authored by Dr Orazi), 3 but rather to provide further evidence that quantification of CD34 þ cells can serve as a useful discriminator between aplastic anemia (AA) and hypoplastic myelodysplastic syndrome (hMDS). Dr Orazi is, in fact, misquoting our paper when he states that we imply that his group did not observe significant differences between the two groups.…”

Reply to Matsui et al.

“…I completely agree with Dr Matsui et al 1 that the enumeration of bone marrow CD34 positive cells should be further tested, possibly in a randomized prospective way, in order to conclusively confirm its validity for separating the two disorders. The fact that the 'CD34 approach' has not become standard of practice up to now, I suspect, it is also due to the usual interlaboratory variability which plagues immunohistochemistry laboratories in general.…”

“…1 The ceramide/S1P rheostat has been proposed as the model to determine the cell's fate. 2 This rheostat is being increasingly recognized as a critical element in tumor cell proliferation and chemotherapy.…”

Estimation of CD34+ in Patients with Hypocellular Marrow and Treatment Response in Aplastic Anemia

“…Accordingly, the percentage of CD34+ cells is usually <0.3% in AA but is either normal or elevated in hMDS. 10 Quantification of marrow CD34+ cells may serve as an important tool for distinguishing between aplastic anemia and hypoplastic myelodysplastic syndrome as shown in few studies. 11,12 In a study …”