Quantitative Analysis of Bioluminescence Optical Signal

Niwa, Kazuki; Kubota, Hidehiro; Enomoto, Toshiteru; Ichino, Yoshiro; Ohmiya, Yoshihiro

doi:10.3390/bios13020223

Cited by 3 publications

(2 citation statements)

References 72 publications

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“…Chemiluminescence arises from the excited state of a species produced by a chemical reaction [ 96 , 97 ]. The method does not require the external excitation light source, thus avoiding the interference from light scattering and background emission from the sample matrix.…”

Section: Redox Cycling-based Optical Bioassaysmentioning

confidence: 99%

Optical Bioassays Based on the Signal Amplification of Redox Cycling

Feng,

Gao,

et al. 2024

Biosensors

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Optical bioassays are challenged by the growing requirements of sensitivity and simplicity. Recent developments in the combination of redox cycling with different optical methods for signal amplification have proven to have tremendous potential for improving analytical performances. In this review, we summarized the advances in optical bioassays based on the signal amplification of redox cycling, including colorimetry, fluorescence, surface-enhanced Raman scattering, chemiluminescence, and electrochemiluminescence. Furthermore, this review highlighted the general principles to effectively couple redox cycling with optical bioassays, and particular attention was focused on current challenges and future opportunities.

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Section: Redox Cycling-based Optical Bioassaysmentioning

confidence: 99%

Optical Bioassays Based on the Signal Amplification of Redox Cycling

Feng,

Gao,

et al. 2024

Biosensors

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“…Бактерицидный эффект проявлялся при 20 мкг/мл. Учитывая, что биолюминесцентный анализ является количественным инструментальным методом [20], он может быть легко адаптирован для проведения высокопроизводительного фармацевтического скрининга растительного сырья на антибактериальную активность.…”

Section: заключениеunclassified

Could Bioluminescent Bacteria be Used in the Search for New Plant-derived Antibacterial Substances?

Katsev,

Safronyuk,

Burtseva

et al. 2023

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. Currently, the search for new antibacterial substances is an urgent task due to the growing resistance of pathogens to existing antibiotics. One of the key directions in this area is the expansion of scientific research of medicinal plants, as new sources of therapeutic agents. This article examines the possibility of using highly sensitive bioluminescent test bacteria for these purposes, which can quickly detect non-specific antimicrobial activity and can be adapted to highly effective pharmaceutical screening technologies.Aim. To study the applicability of bioluminescent bacteria for the analysis of the antibacterial activity of biologically active substances (BAS) of plant origin.Materials and methods. BAS quercetin, 8-hydroxyquinoline, gallic acid and thymoquinone, which are often found in medicinal plant raw materials and with which its antibacterial properties are associated, were used in the work. Bacteria with constitutive bioluminescence Aliivibrio fischeri F1 and Escherichia coli (pXen7), as well as recombinant bioreporter strains with inducible luminescence were used as test-objects: E. coli (pRecA-lux), E. coli (pColD-lux), reacting to nucleic acid damage; E. coli (pKatG-lux) and E. coli (pSoxS-lux), sensitive to oxidative stress.Results and discussion. It was found that the nonspecific antimicrobial activity of the studied BAS is manifested in the inhibition of bacterial bioluminescence of test-strains with constitutive glowing. It was noted that the marine test-bacteria A. fischeri F1 have significantly greater sensitivity to the action of BAS, compared with the recombinant strain of E. coli (pXen7). It has been shown that their inhibitory effect begins at concentrations of 2 mcg/ml, and bactericidal activity occurs at concentrations of more than 20 mcg/ml. The results obtained are compared with the data on MIC and MBC of gram(+) and gram(–) pathogens. The study of the induction of bioluminescence of recombinant bioreporter strains showed that the antibacterial effect of the BAS is accompanied by oxidative stress. Also, quercetin caused activation of luminescence in E. coli (pRecA-lux) and E. coli (pColD-lux), which may indicate its participation in damage to nucleic acids. Analysis of the induction factors of bioreporter strains indicates that the revealed mechanisms of antibacterial activity are not major, but may be of a secondary nature.Conclusion. It has been shown that the intensity of the glow of natural and recombinant bioluminescent bacteria can be an indicator of the antibacterial activity of BAS of natural origin. The high sensitivity of A. fischeri F1 bacteria to the action of substances such as quercetin, 8-hydroxyquinoline, gallic acid and thymoquinone has been shown. Considering that bioluminescence analysis is a quantitative instrumental method, it can be easily adapted for high-throughput pharmaceutical screening. It has been shown that the luminescence intensity of natural and recombinant bioluminescent bacteria can be an indicator of the antibacterial activity of BAS of natural origin. The high sensitivity of A. fischeri F1 to the action of substances such as quercetin, 8-hydroxyquinoline, gallic acid and thymoquinone has been established. Taking in an account that bioluminescent analysis is a quantitative instrumental method, it can be easily adapted for high-throughput pharmaceutical screening.

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Bioluminescence from the bright and dark sides

Kim,

Furuta

2024

Front. Chem. Biol.

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Bioluminescence (BL) is an amazing optical readout that has great potential to be utilized in various bioassays and molecular imaging, but also has some unique defects in practical applications. The recent innovative research on BL has enriched the available repertories of the toolbox. While these efforts greatly diversified the users’ choices in the applications, the wide choices on the contrary do not promise their successful applications to bioassays. This is mainly due to complexity-driven confusion with the diversity and the lack of accurate knowledge on the advantages and disadvantages of BL. This review is intended to showcase the advantages and disadvantages of BL, and serve as a searchlight to find directions for future studies. We hope that this review provides instant references for readers on BL and leads them to properly understand the “bright” and “dark” sides of BL to narrow down their choices in their applications.

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Quantitative Analysis of Bioluminescence Optical Signal

Cited by 3 publications

References 72 publications

Optical Bioassays Based on the Signal Amplification of Redox Cycling

Optical Bioassays Based on the Signal Amplification of Redox Cycling

Could Bioluminescent Bacteria be Used in the Search for New Plant-derived Antibacterial Substances?

Bioluminescence from the bright and dark sides

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