Quantitative analysis of adsorption and desorption behavior of individual cellulase components during the hydrolysis of lignocellulosic biomass with the addition of lysozyme

Toyosawa, Yoshiko; Ikeo, Makoto; Taneda, Daisuke; Okino, Shohei

doi:10.1016/j.biortech.2017.02.132

Cited by 13 publications

(5 citation statements)

References 20 publications

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“…Lysozyme has been shown to enhance the catalytic degradation of lignocellulosic materials when hydrolysis has been carried out with a mixture of cellulase and lysozyme. 34 As protein with no enzymatic activity, lysozyme could enhance the hydrolysis only by adhering to the lignin-rich areas thus reducing the non-specific adsorption of the cellulase. Another study has shown that proteins easily adsorb on lignin surfaces but may still remain hydrated which can be essential for proteins to maintain their structure and functionality.…”

Section: Discussionmentioning

confidence: 99%

Entangled and colloidally stable microcrystalline cellulose matrices in controlled drug release

Dong¹,

Paukkonen

Fang³

et al. 2018

International Journal of Pharmaceutics

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Section: Discussionmentioning

confidence: 99%

Entangled and colloidally stable microcrystalline cellulose matrices in controlled drug release

Dong¹,

Paukkonen

Fang³

et al. 2018

International Journal of Pharmaceutics

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“…Yang and Wyman (2006) reported that only a little amount of BSA was absorbed on pure cellulose while lignin containing substrate adsorbed a significant amount of this additive. Toyosawa et al (2017) showed that the lysozyme adsorbed only to lignin containing substrate and prevent nonproductive adsorption of cellulase by covering the surface of lignin. To improve the efficiency of cellulose conversion, BSA, and lysozyme were added in attempt to block protein adsorption site of lignin and therefore reduce the nonproductive adsorption of enzyme.…”

Section: Resultsmentioning

confidence: 99%

“…Liao et al (2008) proposed dynamic enzyme adsorption based on Langmuir isotherm that incorporated changes of adsorption constant as the cellulose hydrolysis progresses. Toyosawa et al (2017) investigated the effects of noncatalytic protein addition on enzymatic hydrolysis and found that application of lysozyme reduced the nonspecific adsorption of cellulase on lignin and improved enzymatic hydrolysis. However, the kinetic study has not been conducted to describe the relationship between enzyme adsorption and hydrolysis of lignocellulosic substrate with the impacts of enzyme loadings and different additives.…”

Section: Introductionmentioning

confidence: 99%

Construction of a structural enzyme adsorption/kinetics model to elucidate additives associated lignin–cellulase interactions in complex bioconversion system

Chan

Chang

et al. 2021

Biotech & Bioengineering

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Enzymatic hydrolysis is a rate‐limiting process in lignocellulose biorefinery. The reaction involves complex enzyme–substrate and enzyme–lignin interactions in both liquid and solid phases, and has not been well characterized numerically. In this study, a kinetic model was developed to incorporate dynamic enzyme adsorption and product inhibition parameters into hydrolysis simulation. The enzyme adsorption coefficients obtained from Langmuir isotherm were fed dynamically into first‐order kinetics for simulating the equilibrium enzyme adsorption in hydrolysis. A fractal and product inhibition kinetics was introduced and successfully applied to improve the simulation accuracy on adsorbed enzyme and glucose concentrations at different enzyme loadings, lignin contents, and in the presence of bovine serum albumin (BSA) and lysozyme. The model provided numerical proof quantifying the beneficial effects of both additives, which improved the hydrolysis rate by reducing the nonproductive adsorption of enzyme on lignin. The hydrolysis rate coefficient and fractal exponent both increased with increasing enzyme loadings, and lignin inhibition exhibited with increasing fractal exponent. Compared with BSA, the addition of lysozyme exhibited higher hydrolysis rates, which was reflected in the larger hydrolysis rate coefficients and smaller fractal exponents in the simulation. The model provides new insights to support process development, control, and optimization.

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“…Cellulose is a polysaccharide of the β-1,4-glycoside-linked linear chains of glucose. Cellulose contains crystalline and amorphous domains, and several kinds of hydrolytic enzymes are required for its degradation [ 107 ]. An endoglucanase (EG), a β-glucosidase, and a cellobiohydrolase are involved in cellulose degradation.…”

Section: Applications As a Whole-cell Catalyst In Energy Productionmentioning

confidence: 99%

Progress of Molecular Display Technology Using Saccharomyces cerevisiae to Achieve Sustainable Development Goals

Shibasaki

Ueda

2023

Microorganisms

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In the long history of microorganism use, yeasts have been developed as hosts for producing biologically active compounds or for conventional fermentation. Since the introduction of genetic engineering, recombinant proteins have been designed and produced using yeast or bacterial cells. Yeasts have the unique property of expressing genes derived from both prokaryotes and eukaryotes. Saccharomyces cerevisiae is one of the well-studied yeasts in genetic engineering. Recently, molecular display technology, which involves a protein-producing system on the yeast cell surface, has been established. Using this technology, designed proteins can be displayed on the cell surface, and novel abilities are endowed to the host yeast strain. This review summarizes various molecular yeast display technologies and their principles and applications. Moreover, S. cerevisiae laboratory strains generated using molecular display technology for sustainable development are described. Each application of a molecular displayed yeast cell is also associated with the corresponding Sustainable Development Goals of the United Nations.

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Quantitative analysis of adsorption and desorption behavior of individual cellulase components during the hydrolysis of lignocellulosic biomass with the addition of lysozyme

Cited by 13 publications

References 20 publications

Entangled and colloidally stable microcrystalline cellulose matrices in controlled drug release

Entangled and colloidally stable microcrystalline cellulose matrices in controlled drug release

Construction of a structural enzyme adsorption/kinetics model to elucidate additives associated lignin–cellulase interactions in complex bioconversion system

Progress of Molecular Display Technology Using Saccharomyces cerevisiae to Achieve Sustainable Development Goals

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