Quantitative analysis of acankoreoside A and acankoreagenin in the leaves of Schefflera octophylla and Schefflera actinophylla using pressurized liquid extraction and high‐performance liquid chromatography coupled with evaporative light scattering detection

Abstract

A rapid method based on pressurized liquid extraction followed by high-performance liquid chromatography coupled with evaporative light scattering detection was firstly developed for the quantitative analysis of two bioactive triterpenoids (acankoreoside A and acankoreagenin) in the leaves of Schefflera octophylla and Schefflera actinophylla. The analysis was performed on an Agilent Zorbax SB-Aq column (4.6 × 50 mm, 3.5 μm) with gradient elution of 0.1% formic acid and acetonitrile. Calibration curves of two analytes showed good linearity (R(2) > 0.9990) within the tested ranges. This novel method is simple, rapid and accurate, and the results of quantification showed that contents of each investigated compound is significant high in natural S. octophylla (6.36-14.83%), which indicated that natural S. octophylla as potential medicinal resource. Furthermore, hierarchical clustering analysis based on the typical peaks of acankoreoside A and acankoreagenin from the 17 tested samples showed that natural and cultured Schefflera species were in different clusters, which could provide a means of discriminating between Schefflera species from different origins. Thus, acankoreoside A and acankoreagnin could be selected markers for quality control of S. octophylla and S. actinophylla.

3α-Hydroxylup-20(29)-ene-23,28-dioic Acid as a Phytogenic Chemical Marker for Authenticating Schefflera octophylla (Lour.) Harms Monofloral Honey

“…In addition, even in the Schef f lera genus, the distribution of the same compound, such as HLEDA, may exhibit differences to some extent, as Cao et al reported that the content of acankoreoside A showed differences between S. octophylla and S. actinophylla. 10 Additionally, HLEDA was first identified and quantified in honey samples in the present study. As the main triterpenoid from the plant S. octophylla, 57 HLEDA exhibited various functional effects in vitro and in vivo, especially its notable antiinflammatory activity.…”

“…33 Besides, HLEDA and acankoreoside A are the main triterpenoid compounds in P-Sco, which could be recognized as markers for P-Sco quality control. 10 Physicochemical Properties of MH-Sco. Melissopalynological analysis was used to analyze the pollen grains in MH-Sco and their proportion for the first time to preliminarily evaluate the botanical origin because monofloral honey is defined as possessing a specific origin of pollen grains over 45% of the total pollen count.…”

“…9 Owing to the abundant functional components, mainly including triterpenoids, S. octophylla is widely used to relieve rheumatic pain and sore throat as a Chinese medicine formulation and has been evident by pharmacological studies to possess a variety of health benefits, such as antipyretic, antivirus, and anti-inflammatory. 10 Monofloral honey produced from S. octophylla (MH-Sco) is very popular in Southeast China due to its potential medicinal benefits and unique physical properties (e.g., specific herb flavors and a typical bitter aftertaste). Besides, since the flowering phenology of the plant S. octophylla (P-Sco) growing in Southeast China is from November to December in winter, 11 at which there is nectar shortage in the nature, the MH-Sco production is of great economic value in commercial consumption.…”

Acankoreagenin and acankoreosides, a family of lupane triterpenoids with anti‐inflammatory properties: an overview

“…The antinociceptive and anti‐inflammatory activities of S. octophylla extracts have been well characterized, and diverse ACK‐based glycosides (see below) have been isolated from this plant 42–44 . ACK was later found in Schefflera actinophylla , 45,46 in the leaves of Oplopanax horridus , 47 and in the leaves and roots of plants of the Acanthopanax family, such as A. gracilistylus , A. koreanum , and A. trifoliatus (Fig. 4).…”

Chemical characteristics of different parts of Coreopsis tinctoria in China using microwave‐assisted extraction and high‐performance liquid chromatography followed by chemometric analysis

“…However, the comparisons between C. tinctoria and other flower tea materials should also focus on their hydrophilic components, because flavonoids and phenolic acids are the major bioactive constituents in the aqueous extracts of these flower tea materials . Therefore, chemical characteristics are very important for the quality evaluation of different parts of C. tinctoria .…”