Quantitative analyses of plasma opsonizing activity and polymorphonuclear cell response during phagocytosis: standardisation of a chemiluminescent method

Farinelli, Andrea; Setti, Maurizio; Puppo, Francesco; Scudeletti, Marco; Martini, Daniele De; Balestra, Vincenzo; Indiveri, Francesco

doi:10.1111/j.1699-0463.1996.tb04904.x

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…Depending on the particular requirements [8,21] of an assay system whole [20], purified PMNL [I, 2,3,4,7], alveolar macrophages [17], or natural killer cells [10] were used as a source of CL. The in vitro activation of the phagocytic cells was triggered by various stimuli such as: opsonized zymosan [l, 2, 3, 4, 7), or bacteria [18], phorbol myrimethionyl-Ieucyl-phenylalanine [ 6].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of the Chemiluminescence Produced by the Activated Polymorphonuclear Leukocytes in Physiological and Various Pathological Conditions

Olinescu¹,

Greabu²,

Crocnan³

et al. 1998

SBJCHEM

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The chemiluminescent emission released by activated polymorphonuclear leukocytes has been known for a long time, but its clinical use is still scarce. We suggest the as a quantitative measurement of chemiluminescence, the stimulatmy index (ratio of the chemiluminescence value of stimulated polymorphonuclear leukocytes to non-stimulated leukocytes) was obtained for the same individual. The results obtained by a standardized, simplified technique for more than 2000 individuals (healthy persons such as medical personnel, athletes, soldier, and patients suffering from cardiovascular disease, cancer, and pneumoconiosis) explain the still restricted use of chemiluminescence. There was a wide range of individual variations and an overlap of normal physiological and pathological values. A significant statistical difference was obtained only for patients in the acute phase of the disease. The chemiluminescence measurement of phagocytic activity of leukocytes is strongly influenced by age, diet, presence of stress, and chronic inflammations.

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Section: Introductionmentioning

confidence: 99%

Comparative Study of the Chemiluminescence Produced by the Activated Polymorphonuclear Leukocytes in Physiological and Various Pathological Conditions

Olinescu¹,

Greabu²,

Crocnan³

et al. 1998

SBJCHEM

View full text Add to dashboard Cite

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Chemiluminescence and Bioluminescence

Kricka

1999

Anal. Chem.

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The time period covered by this review is the Chemical Abstracts citation dates October 1, 1996, to October 1, 1998. References cited in this review are drawn from the world literature and are limited to the analysis of human fluid and tissues. Chemiluminescence (CL) (light emission produced in a chemical reaction from the decay of chemiexcited species to the electronic ground state) and bioluminescence (BL) (light emission found in nature) have continued to find diverse applications in analysis but only chemiluminescence has become an established technique in routine clinical chemistry.The Proceedings of the ninth International Symposium on Bioluminescence and Chemiluminescence (B1) and a series of review articles (B2-B10) describe recent advances in the analytical applications of CL and BL. The regular literature survey in the Journal of Bioluminescence and Chemiluminescence also provides information on the fundamental and applied aspects of both CL and BL (B11-B14). A survey of commercially available luminometers and imaging devices for low-light level measurements and kits and reagents utilizing BL and CL has been updated (B15).New Reagents and Analytical Reactions. A range of new reagents have been developed for clinical assays and these include CL immunoassay labels such as acridinium esters (B16, B17) and nucleophilic polysubstituted acridinium esters (B18), di-o-bromophenylacridinium esters for use as detection reagents in an amplification cascade immunoassay (alkaline phosphatase label) (B19), acridinium esters for dual-label strategies (B20), fluorene derivatives for enhancing the CL detection of glucose oxidase labels (B21), and pyrylium compounds for detection of doublestranded DNA (B22). Further examples of 1,2-dioxetane derivatives (B23-B26), imidazopyrazinone CL superoxide probes (B27), a range of luminol and isoluminol reagents, including N-(βcarboxypropionyl)luminol (B28) and dimethylisoluminol (B29), and acridine derivatives (B30) have been described.Additional examples of enhancers for CL detection reactions for horseradish peroxidase (HRP) labels have been developed, e.g., phenothiazines (B31), aromatic amine and hydroxy compounds (B32), and boronates (B33, B34). Thiourea enhancers for CL lucigenin reactions (B35) have also been described. Sensitive analytical reactions for β-galactosidase are available using 1,2-dioxetane derivatives or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) (3 amol/assay) (B36). A mutant of Photinus pyralis luciferase (modification of residue 245) with a 5-fold increased K m for ATP has been produced and this improved reagent is useful in ATP assays (B37).Clinical Assays. CL methods continue to be applied to the analysis of body fluids and tissues for clinically important analytes (detection limit): enzymatic-based analysis of apoptotic DNA using CDP-Star (B38), serum oxalate using a coupled oxalate oxidase

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Quantitative analyses of plasma opsonizing activity and polymorphonuclear cell response during phagocytosis: standardisation of a chemiluminescent method

Cited by 2 publications

References 31 publications

Comparative Study of the Chemiluminescence Produced by the Activated Polymorphonuclear Leukocytes in Physiological and Various Pathological Conditions

Comparative Study of the Chemiluminescence Produced by the Activated Polymorphonuclear Leukocytes in Physiological and Various Pathological Conditions

Chemiluminescence and Bioluminescence

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