Quantitative Analyses of Foot Processes, Mitochondria, and Basement Membranes by Structured Illumination

Matsumoto, Ayumi; Matsui, Isao; Katsuma, Yusuke; Yasuda, Seiichi; Shimada, Kazuaki; Namba‐Hamano, Tomoko; Sakaguchi, Yusuke; Kaimori, Jun-Ya; Takabatake, Yoshitsugu; Inoue, Kazunori; Isaka, Yoshitaka

doi:10.1016/j.ekir.2021.04.021

Cited by 7 publications

(4 citation statements)

References 43 publications

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“…MGA is defined as an entity whose glomerular structure shows minor changes on light microscopy, immunostaining, and electron microscopy. 34 Since percutaneous renal biopsy is an invasive procedure and is usually performed for patients with suspected renal disease, it is difficult to obtain normal renal tissues from healthy individuals. Therefore, MGA cases were selected as an alternative to healthy controls as in previous studies by other investigators comparing them with IgA nephropathy cases.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA lnc-CHAF1B-3 promotes renal interstitial fibrosis by regulating EMT-related genes in renal proximal tubular cells

Imai¹,

Ishimoto²,

Doke³

et al. 2023

Molecular Therapy - Nucleic Acids

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Section: Discussionmentioning

confidence: 99%

Long non-coding RNA lnc-CHAF1B-3 promotes renal interstitial fibrosis by regulating EMT-related genes in renal proximal tubular cells

Imai¹,

Ishimoto²,

Doke³

et al. 2023

Molecular Therapy - Nucleic Acids

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“…Extracted protein samples were diluted in 2 × electrophoresis sample buffer (Santa Cruz) containing dithiothreitol. Each sample was subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‒PAGE) using a 10% acrylamide gel and electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Amersham, Germany) 26 . The membrane was cut prior to hybridization to reduce the amount of antibodies used.…”

Section: Methodsmentioning

confidence: 99%

Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats

Yasuda

Inoue

Matsui

et al. 2023

Sci Rep

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Fibroblast growth factor 23, parathyroid hormone, and 1,25-dihydroxyvitamin D are critical in phosphate homeostasis. Despite these factors’ importance, regulators of phosphaturia in the acute postprandial phase remain largely unknown. This study investigated the mechanism of acute phosphate regulation in the postprandial phase in rats. Duodenal administration of radiolabeled phosphate (32P) showed that 32P levels in the inferior vena cava (IVC) blood were lower than those in the portal vein (PV) blood. Serum phosphate concentration transiently increased 5 min after phosphate solution administration through IVC, while it was maintained after the administration through PV. Phosphate administration through both IVC and PV resulted in increased fractional excretion of phosphate (FEPi) at 10 min without elevation of the known circulating factors, but urinary phosphate excretion during the period was 8% of the dose. Experiments using 32P or partial hepatectomy showed that the liver was one of the phosphate reservoirs. The elevation of FEPi and suppression of sodium-phosphate cotransporter 2a in the kidney at 10 min was attenuated in rats with SCH23390, hepatic denervation, or renal denervation, thus indicating that the liver communicated with the kidney via the nervous system to promote phosphaturia. These results revealed previously unknown mechanisms for serum phosphate maintenance.

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“…Kidney sections were viewed under a light microscope after staining, according to the earlier stated technique [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

Protective Role of Betulinic Acid against Cisplatin-Induced Nephrotoxicity and Its Antibacterial Potential toward Uropathogenic Bacteria

Alherz,

Elekhnawy,

Selim

et al. 2023

Pharmaceuticals

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Acute kidney injury (AKI) is one of the major side effects of cisplatin, a remarkable anticancer agent. Therefore, there is a growing need to find an agent that could mitigate cisplatin-induced nephrotoxicity. Betulinic acid (BA) is a natural compound isolated from Silene succulenta Forssk for the first time, with miraculous biological activities and no reports of its effect on the nephrotoxicity induced by cisplatin. Mice received BA orally with doses of 30 and 50 mg/kg before the intraperitoneal injection of cisplatin. Betulinic acid was found to decrease serum levels of creatinine and tissue levels of NGAL and kidney injury molecule (KIM-1) and improve the histological changes in the kidney. In addition, BA decreased the oxidative stress marker malondialdehyde (MDA), increased superoxide dismutase (SOD) antioxidative activity and suppressed the intensity of IL-1B and NFкB immuno-staining. Interestingly, betulinic acid enhanced autophagy by increasing beclin 1, ATG5, and LC3II and decreasing p62 expressions. Thus, our findings suggest betulinic acid as a potential agent that may protect from acute kidney injury by targeting inflammation, oxidative stress, and autophagy processes. Novel drugs are needed to combat the spreading of multidrug resistance between pathogenic bacteria, especially uropathogenic isolates. So, we elucidated the antibacterial properties of BA on Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae. Betulinic acid had minimum inhibitory concentration values (128 to 512 µg/mL). In addition, it adversely affected the membrane integrity of the tested isolates. Accordingly, betulinic acid should be clinically investigated in the future for urinary tract diseases.

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Quantitative Analyses of Foot Processes, Mitochondria, and Basement Membranes by Structured Illumination

Abstract: analyses of foot processes, mitochondria, and basement membranes by structured illumination microscopy using elastica Massonand periodic-acid-Schiff-stained kidney sections, Kidney International Reports (2021), doi:

Cited by 7 publications

References 43 publications

Long non-coding RNA lnc-CHAF1B-3 promotes renal interstitial fibrosis by regulating EMT-related genes in renal proximal tubular cells

Long non-coding RNA lnc-CHAF1B-3 promotes renal interstitial fibrosis by regulating EMT-related genes in renal proximal tubular cells

Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats

Protective Role of Betulinic Acid against Cisplatin-Induced Nephrotoxicity and Its Antibacterial Potential toward Uropathogenic Bacteria

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