Quantitative analyses of amount and localization of radiosensitizer gold nanoparticles interacting with cancer cells to optimize radiation therapy

Hatoyama, Keiichiro; Kitamura, Narufumi; Takano-Kasuya, Mayumi; Tokunaga, Masayuki; Oikawa, Takahiro; Ohta, M.; Hamada, Yo; Tada, Hiroshi; Kobayashi, Yoshio; Kamei, Takashi; Gonda, Kohsuke

doi:10.1016/j.bbrc.2018.12.016

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…However, NP delivery is very heterogenous (Carmona et al, 2017; T. Liu, Kempson, De Jonge, Howard, & Thierry, 2014; C. H. Wang et al, 2011). Based on the work by Hatoyama et al (2019), 5.14 × 10 4 particles per cell was the minimum amount of GNPs needed in their study to induce radiosensitization under 3 Gy X‐ray irradiation. However nanoparticle uptake in a cell population is highly heterogenous between cells, spanning up to 4 orders of magnitude according to Turnbull, Thierry, and Kempson (2019) and that the low numbers of GNPs can lead to a radioprotective effect.…”

Section: Discussionmentioning

confidence: 99%

“…Hatoyama et al (2019) reported on anti‐human epidermal growth factor receptor type 2 (HER2) GNPs cocultured with SK‐OV3, and irradiated with an 80 kV source with 3 or 5 Gy. Surprisingly, at 0 and 24 hr of co‐cultures when the nanoparticles were either membrane associated, or internalized via endocytosis respectively, there was comparable radiosensitization achieved relative to control for both conditions.…”

Section: Radiobiological Diversion From Physical Theorymentioning

confidence: 99%

“…For example, the physical processes of dose deposition for a nanoparticle is well described by theory, however the extent of protein adsorption, cellular uptake, intracellular localization can all vary even between apparently similar nanoparticles, so while the physical process may be comparable in dose deposition, confounding biological variables can vary greatly. Hatoyama et al (2019) reported on anti-human epidermal growth factor receptor type 2 (HER2) GNPs cocultured with SK-OV3, and irradiated with an 80 kV source with 3 or 5 Gy. Surprisingly, at 0 and 24 hr of co-cultures when the nanoparticles were either membrane associated, or internalized via endocytosis respectively, there was comparable radiosensitization achieved relative to control for both conditions.…”

Section: Clinical Statusmentioning

confidence: 99%

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Mechanisms of nanoparticle radiosensitization

Kempson

2020

WIREs Nanomed Nanobiotechnol

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Metal‐based nanoparticles applied to potentiating the effects of radiotherapy have drawn significant attention from the research community and are now available clinically. By improving our mechanistic understanding, nanoparticles are likely to evolve to provide very significant improvements in radiotherapy outcomes with only incremental increase in cost. This review critically assesses the inconsistent observations surrounding physical, physicochemical, chemical and biological mechanisms of radiosensitization. In doing so, a number of needs are identified for continuing research and are highlighted. The large degree of variability from one nanoparticle to another emphasizes that it is a mistake to generalize nanoparticle radiosensitizer mechanisms. Nanoparticle formulations should be considered in an analogous way as pharmacological agents and as a broad class of therapeutic agents, needing to be considered with a high degree of individuality with respect to their interactions and ultimate impact on radiobiological response. In the same way that no universal anti‐cancer drug exists, it is unlikely that a single nanoparticle formulation will lead to the best therapeutic outcomes for all cancers. The high degree of complexity and variability in mechanistic action provides notable opportunities for nanoparticle formulations to be optimized for specific indications. This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies Nanotechnology Approaches to Biology > Nanoscale Systems in Biology

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Section: Discussionmentioning

confidence: 99%

Section: Radiobiological Diversion From Physical Theorymentioning

confidence: 99%

Section: Clinical Statusmentioning

confidence: 99%

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Mechanisms of nanoparticle radiosensitization

Kempson

2020

WIREs Nanomed Nanobiotechnol

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“…104,105 The radiosensitizing effect of HER2 antibody-decorated GNPs was also confirmed in an ovarian cancer cell line (SK-OV3) . 106 Similarly, Popovtzer et al 107 used cetuximab to target EGFR, allowing GNPs to selectively target head and neck tumors and significantly increase the radiation dose absorbed in tumor tissues. When combined with radiotherapy (25 Gy,6 MV,1.4 Gy/min), a significant tumorgrowth delay in tumor-bearing mice was observed.…”

mentioning

confidence: 99%

<p>Gold Nanoparticles as Radiosensitizers in Cancer Radiotherapy</p>

Chen

Yang

Shao

et al. 2020

IJN

178

191

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The rapid development of nanotechnology offers a variety of potential therapeutic strategies for cancer treatment. High atomic element nanomaterials are often utilized as radiosensitizers due to their unique photoelectric decay characteristics. Among them, gold nanoparticles (GNPs) are one of the most widely investigated and are considered to be an ideal radiosensitizers for radiotherapy due to their high X-ray absorption and unique physicochemical properties. Over the last few decades, multidisciplinary studies have focused on the design and optimization of GNPs to achieve greater dosing capability and higher therapeutic effects and highlight potential mechanisms for radiosensitization of GNPs. Although the radiosensitizing potential of GNPs has been widely recognized, its clinical translation still faces many challenges. This review analyses the different roles of GNPs as radiosensitizers in cancer radiotherapy and summarizes recent advances. In addition, the underlying mechanisms of GNP radiosensitization, including physical, chemical and biological mechanisms are discussed, which may provide new directions for the optimization and clinical transformation of next-generation GNPs.

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“…On the basis of TEM images, the intracellular biodistribution of AuNPs tested in cell cytoplasm was demonstrated. Size, shape, surface chemistry, and surface modifications of AuNPs have an important role in biodistribution [ 10 , 17 , 37 , 38 ]. A recent study indicates that mitochondrial damage also presents a risk to long-term cancer cell growth [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines

Soares

Faria

Aires

et al. 2023

IJMS

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More than 50% of all prostate cancer (PCa) patients are treated by radiotherapy (RT). Radioresistance and cancer recurrence are two consequences of the therapy and are related to dose heterogeneity and non-selectivity between normal and tumoral cells. Gold nanoparticles (AuNPs) could be used as potential radiosensitizers to overcome these therapeutic limitations of RT. This study assessed the biological interaction of different morphologies of AuNPs with ionizing radiation (IR) in PCa cells. To achieve that aim, three different amine-pegylated AuNPs were synthesized with distinct sizes and shapes (spherical, AuNPsp-PEG, star, AuNPst-PEG, and rods, AuNPr-PEG) and viability, injury and colony assays were used to analyze their biological effect on PCa cells (PC3, DU145, and LNCaP) when submitted to the accumulative fraction of RT. The combinatory effect of AuNPs with IR decreased cell viability and increased apoptosis compared to cells treated only with IR or untreated cells. Additionally, our results showed an increase in the sensitization enhancement ratio by cells treated with AuNPs and IR, and this effect is cell line dependent. Our findings support that the design of AuNPs modulated their cellular behavior and suggested that AuNPs could improve the RT efficacy in PCa cells.

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Quantitative analyses of amount and localization of radiosensitizer gold nanoparticles interacting with cancer cells to optimize radiation therapy

Cited by 11 publications

References 19 publications

Mechanisms of nanoparticle radiosensitization

Mechanisms of nanoparticle radiosensitization

<p>Gold Nanoparticles as Radiosensitizers in Cancer Radiotherapy</p>

Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines

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