Quantitative Alterations of Capillary Diameter Have a Predictive Value for Development of the Capillaroscopic Systemic Sclerosis Pattern

Trombetta, Amelia Chiara; Smith, Vanessa; Pizzorni, Carmen; Meroni, M.; Paolino, Sabrina; Cariti, Caterina; Ruaro, Barbara; Sulli, Alberto; Cutolo, Maurizio

doi:10.3899/jrheum.150900

Cited by 45 publications

(51 citation statements)

References 33 publications

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“…Maximal capillary diameter was calculated from bright-field microscopy images as the largest diameter of the erythrocyte column measured from the arterial or venous limb of a capillary loop or the apex of the loop. A capillary diameter greater than 20 μm is considered to be elevated, and capillaries with diameters greater than 50 μm are defined as “giant” and are associated with development of SSc [ 22 23 ].…”

Section: Methodsmentioning

confidence: 99%

Assessing nailfold microvascular structure with ultra-wideband raster-scan optoacoustic mesoscopy

et al. 2018

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Nailfold capillaroscopy, based on bright-field microscopy, is widely used to diagnose systemic sclerosis (SSc). However it cannot reveal information about venules and arterioles lying deep under the nailfold, nor can it provide detailed data about surface microvasculature when the skin around the nail is thick. These limitations reflect the fact that capillaroscopy is based on microscopy methods whose penetration depth is restricted to about 200 μm. We investigated whether ultra-wideband raster-scan optoacoustic mesoscopy (UWB-RSOM) can resolve small capillaries of the nailfold in healthy volunteers and compared the optoacoustic data to conventional capillaroscopy examinations. We quantified UWB-RSOM-resolved capillary density and capillary diameter as features that relate to SSc biomarkers, and we obtained the first three-dimensional, in vivo images of the deeper arterioles and venules. These results establish the potential of UWB-RSOM for analyzing SSc-relevant markers.

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Section: Methodsmentioning

confidence: 99%

Assessing nailfold microvascular structure with ultra-wideband raster-scan optoacoustic mesoscopy

et al. 2018

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“…Quantitative capillaroscopic parameters were recently confirmed of value in prediction of disease progression. [28][29] We are therefore optimistic that, through a combination of standardised training and increased use of automated analysis [30], the non-invasive technique of videocapillaroscopy will ultimately deliver as an outcome measure of SSc-related microangiopathy.…”

Section: Discussionmentioning

confidence: 99%

Intra-and inter-observer reliability of nailfold videocapillaroscopy — A possible outcome measure for systemic sclerosis-related microangiopathy

Dinsdale

Moore

O’Leary

et al. 2017

Microvascular Research

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“…Capillary absolute number was obtained counting all capillary loops, observable in the first row, in 1 mm per field, over 32 NVC fields (8 fingers, 4 fields per finger, fingers 2-5 of each hand). Other nailfold capillaries abnormalities were scored by a validated semiquantitative rating scale, in accordance with previous studies 20,21,22 .…”

Section: Methodsmentioning

confidence: 99%

Effects of Longterm Treatment with Bosentan and Iloprost on Nailfold Absolute Capillary Number, Fingertip Blood Perfusion, and Clinical Status in Systemic Sclerosis

Trombetta

Pizzorni²,

Ruaro³

et al. 2016

J Rheumatol

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Objective.To quantify in patients with systemic sclerosis (SSc) the absolute nailfold capillary number/mm (the absolute number of capillaries, observable in the first row, in 1 mm per field) and fingertip blood perfusion (FBP) during longterm therapy with the endothelin receptor antagonist bosentan (BOSE) and the synthetic analog of prostacyclin PGI2 iloprost (ILO) by multiple diagnostic tools. Observed values were correlated with clinical outcomes.Methods.Thirty patients with SSc already receiving intravenous ILO (80 μg/day) for 5 continuous days (every 3 mos) were recruited in the clinic. Fifteen patients continued such treatment (ILO group), while in 15 patients BOSE (125 mg twice/day) was added (ILO + BOSE group) because of the onset of pulmonary arterial hypertension or digital ulcers (DU). The followup period was 4 years (T0–T4). Every year the following were evaluated: absolute nailfold capillary number/mm by nailfold videocapillaroscopy, FBP by laser Doppler flowmetry, DU incidence, DLCO, systolic pulmonary arterial pressure (sPAP), renal arterial resistive index, and other biomarkers. From T2 to T4, laser speckled contrast analysis was added. Nonparametric tests were used for statistical analysis.Results.Limited to the ILO + BOSE group, absolute capillary number/mm and FBP showed a progressive increase independently from other variables. In addition, during followup there was a significant reduction (80%) in the incidence of new DU, whereas DLCO and sPAP did not worsen.Conclusion.The study shows in patients with SSc with up to 4 years of combined therapy a progressive significant recovery in structure and function of microvasculature linked to improved clinical outcomes, independent of disease severity.

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Quantitative Alterations of Capillary Diameter Have a Predictive Value for Development of the Capillaroscopic Systemic Sclerosis Pattern

Cited by 45 publications

References 33 publications

Assessing nailfold microvascular structure with ultra-wideband raster-scan optoacoustic mesoscopy

Assessing nailfold microvascular structure with ultra-wideband raster-scan optoacoustic mesoscopy

Intra-and inter-observer reliability of nailfold videocapillaroscopy — A possible outcome measure for systemic sclerosis-related microangiopathy

Effects of Longterm Treatment with Bosentan and Iloprost on Nailfold Absolute Capillary Number, Fingertip Blood Perfusion, and Clinical Status in Systemic Sclerosis

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