Quantitative 2D HSQC NMR Analysis of Mixed Wood Sugars in Hemicellulosic Hydrolysate Fermentation Broth

Production of (R)-3-hydroxybutyric acid by Burkholderia cepacia from wood extract hydrolysates

“…A modified two dimensional (2D) heteronuclear single quantum coherence (HSQC) method was used to determine the sugar concentration (Shupe et al [2012]; Shupe and Liu [2012a]).…”

Hot Water Pretreatment of Boreal Aspen Woodchips in a Pilot Scale Digester

“…Minor compounds, including furfural, acetate group, hydroxymethylfurfural (HMF), and formic acid were determined by proton NMR spectroscopy (Bruker AVANCE III 600 MHz, Bruker Biospin Corporation, Billerica, MA, USA) [28], while the heteronuclear single quantum coherence spectroscopy (2D-HSQC, Bruker AVANCE III 600 MHz) [29] was employed to determine the monosaccharides and oligosaccharides obtained during the extraction. The internal standard solution consisted of D2O (95.532%), trimethylamine hydrochloride (TMA) (0.2281%), 3-(trimethylsilys) propionic-2,2,3,3-d4 acid (TSP) (0.05304%), and glucosamine (4.187%).…”

“…All the other peak integrals are normalized to α-C1H1 for glucosamine. Either α-anomeric C1H1 peak or together with β-anomeric C1H1 peak can be used to calculate the concentration of monosaccharides [29]. Both data from proton NMR and 2D-HSQC were analyzed using Mestranova NMR software.…”

“…The formation rate of oligo-saccharides based on number of moles is given by: (29) where C0 0 C is the xylo-oligomer mole concentration. Figure 5 show the variations of xylose, xylooligomer, total xylose units, and furfural during the hot water extraction.…”

Quantification of xylooligomers in hot water wood extract by 1H–13C heteronuclear single quantum coherence NMR

“…With outstanding qualities (Wang & Liu, 2012), 31 hot water extraction is a popular pretreatment method to extract useful components from 32 woody biomass. During hot water extraction, most of hemicelluloses and extractives, as 33 well as a small amount of cellulose and lignin, are extracted from woody biomass (Liu, Chromatography (Sjöström & Alen, 1999), Gas Chromatography/ Mass Spectrometry 41 (Sjöström & Alen, 1999), Proton NMR (1D NMR) (Kiemle et al, 2004), or 42 Heteronuclear Single Quantum Coherence NMR (2D-HSQC) (Shupe et al, 2012). 43 Compared to the other methods, 2D-HSQC technique has unique merits, such as easy 44 sample preparation, fast determination, and most importantly provide a second dimension 45 to the spectrum which enables the data easier to be interpreted.…”