Quantitation of the niacin metabolites 1-methylnicotinamide and l-methyl-2-pyridone-5-carboxamide in random spot urine samples, by ion-pairing reverse-phase HPLC with UV detection, and the implications for the use of spot urine samples in the assessment of niacin status

Creeke, Paul I.; Seal, Andrew

doi:10.1016/j.jchromb.2004.12.012

Cited by 24 publications

(12 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the ratio of these metabolites has been shown to vary according to the time after the last meal because they are sequential intermediates on the same catabolic pathway (21). This makes the ratio an intrinsically unstable variable for use in population surveys; in the present study we chose to use cutoffs previously established for the excretion of individual metabolites expressed relative to creatinine.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Low and deficient niacin status and pellagra are endemic in postwar Angola

Seal

Creeke

Dibari

et al. 2007

The American Journal of Clinical Nutrition

View full text Add to dashboard Cite

Background: Outbreaks of pellagra were documented during the civil war in Angola, but no contemporary data on the incidence of pellagra or the prevalence of niacin deficiency were available. Objective: The objective was to investigate the incidence of pellagra and the prevalence of niacin deficiency in postwar Angola and their relation with dietary intake, poverty, and anthropometric status. Design: Admissions data from 1999 to 2004 from the pellagra treatment clinic in Kuito, Angola, were analyzed. New patients admitted over 1 wk were examined, and urine and blood samples were collected. A multistage cluster population survey collected data on anthropometric measures, household dietary intakes, socioeconomic status, and clinical signs of pellagra for women and children. Urinary excretion of 1-methylnicotinamide, 1-methyl-2-pyridone-5-carboxymide, and creatinine was measured and hemoglobin concentrations were measured with a portable photometer. Results: The incidence of clinical pellagra has not decreased since the end of the civil war in 2002. Low excretion of niacin metabolites was confirmed in 10 of 11 new clinic patients. Survey data were collected for 723 women aged 15-49 y and for 690 children aged 6 -59 mo. Excretion of niacin metabolites was low in 29.4% of the women and 6.0% of the children, and the creatinine-adjusted concentrations were significantly lower in the women than in the children (P 0.001, t test). In children, niacin status was positively correlated with the household consumption of peanuts (r ҃ 0.374, P ҃ 0.001) and eggs (r ҃ 0.290, P ҃ 0.012) but negatively correlated with socioeconomic status (r ҃ Ҁ0.228, P ҃ 0.037). Conclusions: The expected decrease in pellagra incidence after the end of the civil war has not occurred. The identification of niacin deficiency as a public health problem should refocus attention on this nutritional deficiency in Angola and other areas of Africa where maize is the staple.Am J Clin Nutr 2007;85:218 -24.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The urinary niacin metabolites n-methyl nicotinamide (1-MN) and 2-pyridone 5-carboxymide (2-PYR) were measured by ionpairing reversed-phase HPLC with ultraviolet diode array detection (21). The limits of detection for 1-MN and 2-PYR were 0.5 and 0.1 mol/L respectively.…”

Section: Collection Of Urine Samples and Biochemical Analysismentioning

confidence: 99%

Low and deficient niacin status and pellagra are endemic in postwar Angola

Seal

Creeke

Dibari

et al. 2007

The American Journal of Clinical Nutrition

View full text Add to dashboard Cite

show abstract

“…The chromatographic separation of niacin and its metabolites is possible by reversed‐phase HPLC, hydrophilic liquid interaction chromatography, normal‐phase HPLC, and supercritical fluid chromatography . Detection is achieved by UV absorption, MS, or MS/MS …”

Section: Introductionmentioning

confidence: 99%

Urinary Excretion of Niacin Metabolites in Humans After Coffee Consumption

Kremer

Gömpel

Bakuradze

et al. 2018

Molecular Nutrition Food Res

View full text Add to dashboard Cite

ScopeCoffee is a major natural source of niacin in the human diet, as it is formed during coffee roasting from the alkaloid trigonelline. The intention of our study was to monitor the urinary excretion of niacin metabolites after coffee consumption under controlled diet.Methods and resultsWe performed a 4‐day human intervention study on the excretion of major niacin metabolites in the urine of volunteers after ingestion of 500 mL regular coffee containing 34.8 μmol nicotinic acid (NA) and 0.58 μmol nicotinamide (NAM). In addition to NA and NAM, the metabolites N 1‐methylnicotinamide (NMNAM), N 1‐methyl‐2‐pyridone‐5‐carboxamide (2‐Py), and nicotinuric acid (NUA) were identified and quantified in the collected urine samples by stable isotope dilution analysis (SIVA) using HPLC‐ESI‐MS/MS. Rapid urinary excretion was observed for the main metabolites (NA, NAM, NMNAM, and 2‐Py), with t max values within the first hour after ingestion. NUA appeared in traces even more rapidly. In sum, 972 nmol h−1 of NA, NAM, NMNAM, and 2‐Py were excreted within 12 h after coffee consumption, corresponding to 6% of the ingested NA and NAM.ConclusionThe results indicate regular coffee consumption to be a source of niacin in human diet.

show abstract

“…NA (niacinamide) is the amide of nicotinic acid (niacin, vitamin PP or B 3 ) [ 4 ]. Over the years, NA has been tried in the treatment of various diseases, such as pellagra, psoriasis, diabetes, and schizophrenia [ 12 , 21 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

1-Methylnicotinamide and nicotinamide: two related anti-inflammatory agents that differentially affect the functions of activated macrophages

Biedroń

Ciszek

Tokarczyk

et al. 2008

Arch. Immunol. Ther. Exp.

View full text Add to dashboard Cite

Introduction: 1-Methylnicotinamide (MNA), a major metabolite of nicotinamide (NA), is known to exert anti-inflammatory effects in vivo. Treatment of inflammatory skin diseases by topical application of MNA provides certain advantages over the use of NA. However, in contrast to NA, the molecular mechanisms of the anti-inflammatory properties of MNA are not well known. In this study the influence of exogenous MNA and NA in vitro on the generation of inflammatory mediators by macrophages (Mφ) was investigated. Materials and Methods: Peritoneal Mφ of CBA/J mice were activated in vitro with lipopolysaccharide and incubated with MNA or NA. The effect of these compounds on biological functions of Mφ was measured by evaluation of the production of reactive oxygen species (ROS) by luminol-dependent chemiluminescence, cytokines and prostaglandin E 2 (PGE 2 ) by ELISA, and nitric oxide (NO) by the Griess method. Moreover, the expressions of inducible NO synthase and cyclooxygenase-2 were measured by Western blotting. Results: It was shown that at non-cytotoxic concentrations, NA inhibits the production of a variety of pro-inflammatory agents, such as tumor necrosis factor α, interleukin 6, NO, PGE 2 , and the generation of ROS. In contrast to NA, exogenous MNA inhibited only the generation of ROS, while its effect on the synthesis of other mediators was negligible. Conclusions: These results indicate that the anti-inflammatory properties of MNA demonstrated previously in vivo do not depend on its capacity to suppress the functions of immune cells, but more likely may be related to its action on vascular endothelium. The authors suggest that the limited permeability for exogenous MNA, in contrast to that for NA, may be responsible for its lack of suppressor activity against Mφ.

show abstract

Quantitation of the niacin metabolites 1-methylnicotinamide and l-methyl-2-pyridone-5-carboxamide in random spot urine samples, by ion-pairing reverse-phase HPLC with UV detection, and the implications for the use of spot urine samples in the assessment of niacin status

Cited by 24 publications

References 21 publications

Low and deficient niacin status and pellagra are endemic in postwar Angola

Low and deficient niacin status and pellagra are endemic in postwar Angola

Urinary Excretion of Niacin Metabolites in Humans After Coffee Consumption

1-Methylnicotinamide and nicotinamide: two related anti-inflammatory agents that differentially affect the functions of activated macrophages

Contact Info

Product

Resources

About