Quantitation of suspected allergens in fragrances (Part I): evaluation of comprehensive two‐dimensional gas chromatography for quality control

Abstract

An evaluation of comprehensive two-dimensional (2D) gas chromatography (GC×GC) was performed to assess its suitability for the analysis of volatile fragrance components, recognized by the European Commission's Scientific Committee on Cosmetics and other Non-food Products (SCCNFP) as possible skin sensitizers. The 24 volatile components listed by the SCCNFP were baseline-resolved or better within one 30 min analysis. Highquality calibration data for standard mixtures were obtained, with R 2 > 0.998 over the concentration range 2-1000 mg/l. However, the analysis of small spiked amounts of target compounds in truly complex fragrances was problematic, due to uncertainty in component assignment. The benefits and limitations of GC×GC are reported, and a discussion of the proposed directions for the solution of this analysis is provided.

Evaluation of different internal‐diameter column combinations in comprehensive two‐dimensional gas chromatography in flavour and fragrance analysis

“…The specificity of the method is a critical step in a validation procedure, and in particular for target analytes quantitation [24,25] and is one of the primary goals that can fruitfully be reached by proper selectivity tuning of the 2-D column stationary phase [26,27]. Widely used orthogonal stationary phase combinations, such as 1-D OV1/2-D CW20 M, are not effective enough in this connection, in particular with polar analytes (alcohols and some carbonyl-substituted derivatives) affected by a strong 2-D retention, mainly due to the inappropriate elution temperature of the 2-D column.…”

Multidimensional and Comprehensive Two-Dimensional Gas Chromatography

“…Routine GC-MS can be used to analyse 24 out of these 26 allergens in consumer (and intermediate) products, but insufficient peak capacity and peak-shifting easily cause problems. On the other hand, GCÂGC on SPB-1 Â SupelcoWax-10 yields a satisfactory separation of all 24 allergens in a mere 30 min [94]. A baseline separation was effected for the often occurring α-and β-ionone from each other and from trace-level α-isomethylionone.…”

Cromatografia gasosa bidimensional abrangente (GC × GC)

“…[72][73][74][75][76][77][78] Na separação de voláteis de cachaça 79 foi relatada a possibilidade distinguir o tipo de madeira empregada no processo de envelhecimento; já para voláteis de uva 80 o perfil dos terpenos e seus derivados oxigenados pode ser empregado para traçar a origem da uva. Ainda, a separação e quantificação de alergênicos 46,47,[81][82][83] presentes em perfumes e cosméticos, bem como em óleos essenciais, também tem sido bastante investigada por GC x GC.…”