Quantitation of Selenomethionine in Multivitamins and Selenium Supplements by High Performance Liquid Chromatography Inductively-Coupled Plasma Mass Spectrometry

LeBlanc, Kelly L.; Kawamoto, Mayumi; Le, Phuong-Mai; Grinberg, Patrícia; Nadeau, Kenny; Yang, Lu; Nogueira, A. R. de A.; Mester, Zoltán

doi:10.1007/s12161-019-01442-6

Cited by 10 publications

(3 citation statements)

References 39 publications

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“…The separation efficiency and separation time of specific substances by different HPLC columns vary depending on different pore sizes, particle sizes, and fillers. The classical AE column was typically used for the separation of Se compounds from various plants, , while the polar-modified RP column was used to separate polar compounds with 100% aqueous solutions and ion-pair reagents as the mobile phase. , In this study, two HPLC separation methods were established using anion exchange column (column 1) and hydrophobic column (column 2). All five selenides (SeCys 2 , MeSeCys, SeMet, SeIV, and SeVI) could be completely separated within 15 min under two chromatographic conditions (Figure S1A,B).…”

Section: Resultsmentioning

confidence: 99%

Precise Determination of Selenium Forms and Contents in Selenium-Enriched Rapeseed Seedlings and Flowering Stalks by HPLC-ICP-MS

Zhan,

Qi,

Wang

et al. 2024

J. Agric. Food Chem.

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Rapeseed (Brassica napus L.) has the ability of selenium (Se) enrichment. Identification of selenides in Se-rich rapeseed products will promote the development and utilization of high value. By optimizing the Se species extraction process (protease type, extraction reagent, enzyme sample ratio, extraction time, etc.) and chromatographic column, an efficient, stable, and accurate method was established for the identification of Se species and content in rapeseed seedlings and flowering stalks, which were cultured by inorganic Se hydroponics. Five Se compounds, including selenocystine (SeCys 2 ), methylselenocysteine (MeSeCys), selenomethionine (SeMet), selenite (SeIV), and selenate (SeVI) were qualitatively and quantitatively identified. Organoselenium was absolutely dominant in both seedlings and flowering stalks among the detected rapeseed varieties, with 64.18− 90.20% and 94.38−98.47%, respectively. Further, MeSeCys, a highly active selenide, predominated in rapeseed flowering stalks with a proportion of 56.36−72.93% and a content of 1707.3−5030.3 μg/kg. This study provides a new source of MeSeCys supplementation for human Se fortification.

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Section: Resultsmentioning

confidence: 99%

Precise Determination of Selenium Forms and Contents in Selenium-Enriched Rapeseed Seedlings and Flowering Stalks by HPLC-ICP-MS

Zhan,

Qi,

Wang

et al. 2024

J. Agric. Food Chem.

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“…This demonstrates that the limiting factor is at the lower end of the concentration range rather than the upper end. For samples where levels of SeMet are significantly lower, such as in dietary supplements (where selenized yeast is frequently used as the source of Se 26 ), significantly smaller quantities of SEEY-1 would be required, and 80 Se would be the ideal isotope to use for analysis.…”

Section: Resultsmentioning

confidence: 99%

⁸²Se Metabolically-Labeled Yeast as a Matrix-Matched Isotope Dilution Standard for Quantification of Selenomethionine

LeBlanc,

Hörndli,

Bergeron

et al. 2023

Anal. Chem.

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Selenized yeast is commonly used as a highly bioavailable source of selenium in dietary supplements and feed additives and is used in research settings in various disciplines due to the large number of selenium-containing metabolites formed during growth. With the selenomethionine being the major form of selenium present in selenized yeasts, its accurate quantitation is essential, however, values are frequently underestimated due to the costly and time-consuming hydrolysis-based sample preparation required to release the selenoamino acid from proteins for analysis.

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“…Ion-exchange chromatography is based on the exchange equilibrium between charged solute ions and the oppositely charged site on the stationary phase with ion exchange resin [23,27]. The retention of the target anion is determined by the nature of the competing ion and its concentration as well as the mobile phase pH [28].…”

Section: Discussionmentioning

confidence: 99%

Separation of selenium species in plant tissues by high performance liquid chromatography-ultraviolet treatment-hydride generation atomic fluorescence spectrometry using various mobile phases

Han

Xiong

Jia

et al. 2021

Biotechnology & Biotechnological Equipment

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Anion-exchange chromatography (Hamilton, PRP-X100) with hydride-generation atomic fluorescence spectrometry (HG-AFS) is commonly used for selenium (Se) speciation analysis in environmental and biological samples. However, retentions of Se species are often different with different mobile phases. To address it, this study systematically investigated mobile phases based on ammonium salts to obtain good resolution of Se species. Various mobile phases, including NH 4 H 2 PO 4 , NH 4 Ac, NH 4 NO 3 and (NH 4 ) 2 HPO 4 , were tested. The best result was obtained with a mobile phase containing 60 mmol L −1 (NH 4 ) 2 HPO 4 at pH 6.0, where the separation of Se species, including selenite [Se(IV)], selenate [Se(VI)], selenocystine (SeCys) and selenomethionine (SeMet), was achieved within 10 min with reasonable resolution. In addition, the recoveries of SeCys, Se (IV), SeMet and Se (VI) in the Se-enriched samples were 95.72 ± 1.60%, 106.89 ± 2.20%, 93.28 ± 2.82% and 91.38 ± 1.27%, respectively. Finally, the proposed method was validated for the determination of Se species in Nicotiana tabacum tissues.

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Quantitation of Selenomethionine in Multivitamins and Selenium Supplements by High Performance Liquid Chromatography Inductively-Coupled Plasma Mass Spectrometry

Cited by 10 publications

References 39 publications

Precise Determination of Selenium Forms and Contents in Selenium-Enriched Rapeseed Seedlings and Flowering Stalks by HPLC-ICP-MS

Precise Determination of Selenium Forms and Contents in Selenium-Enriched Rapeseed Seedlings and Flowering Stalks by HPLC-ICP-MS

⁸²Se Metabolically-Labeled Yeast as a Matrix-Matched Isotope Dilution Standard for Quantification of Selenomethionine

Separation of selenium species in plant tissues by high performance liquid chromatography-ultraviolet treatment-hydride generation atomic fluorescence spectrometry using various mobile phases

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Quantitation of Selenomethionine in Multivitamins and Selenium Supplements by High Performance Liquid Chromatography Inductively-Coupled Plasma Mass Spectrometry

Cited by 10 publications

References 39 publications

Precise Determination of Selenium Forms and Contents in Selenium-Enriched Rapeseed Seedlings and Flowering Stalks by HPLC-ICP-MS

Precise Determination of Selenium Forms and Contents in Selenium-Enriched Rapeseed Seedlings and Flowering Stalks by HPLC-ICP-MS

82Se Metabolically-Labeled Yeast as a Matrix-Matched Isotope Dilution Standard for Quantification of Selenomethionine

Separation of selenium species in plant tissues by high performance liquid chromatography-ultraviolet treatment-hydride generation atomic fluorescence spectrometry using various mobile phases

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⁸²Se Metabolically-Labeled Yeast as a Matrix-Matched Isotope Dilution Standard for Quantification of Selenomethionine