Quantitation of residual WBCs in filtered blood components by high‐throughput, real‐time kinetic PCR

Abstract

kPCR is a high-throughput, sensitive assay that could prove useful in routine quality assurance of the WBC reduction process.

Enhanced ascertainment of microchimerism with real‐time quantitative polymerase chain reaction amplification of insertion‐deletion polymorphisms

“…Blood samples were aliquotted and frozen as previously described. 10 Frozen whole-blood samples were thawed and microcentrifuged at 2000 × g (Eppendorf, Westbury, NY) for 5 minutes. The supernatant was aspirated, leaving a white blood cell (WBC) pellet.…”

Signs and symptoms associated with the transfusion of WBC‐reduced RBCs and non‐WBC‐reduced RBCs in patients with anemia and HIV infection: results from the Viral Activation Transfusion Study

“…Quality control of residual WBC content of WBC-reduced RBCs and platelets was as previously described. 48,49 All blood suppliers were required to submit quality-control data regarding WBC reduction, and the number of residual WBCs in each unit transfused was independently determined by the VATS Central Laboratory. Of the 1869 WBC-reduced units of WBCs for which data are available, 15 units (0.8%) from 9 of the 11 sites had a residual WBC count of >5 ‫ן‬ 10 6 WBCs per unit.…”

Evaluation of droplet digital PCR for quantification of residual leucocytes in red blood cell concentrates

“…A PCR method for detection of rWBC in blood components would be an attractive alternative to flow‐cytometry due to the potential to perform quality controls in batches. In 2002, Lee and coworkers published a high‐throughput real‐time PCR for determination of rWBC in blood products. Quantitative PCR appears to be rarely applied for enumeration of rWBC although established in many laboratories for other parameters.…”