Quantitation of Red Blood Cell‐bound C3d in Normal Subjects and Random Hospitalized Patients

Chaplin, Hugh; Nasongkla, M.; Monroe, Martha C.

doi:10.1111/j.1365-2141.1981.00069.x

Cited by 49 publications

(19 citation statements)

References 9 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…17 In contrast to this finding is the fact that several studies of hospitalized patients have demonstrated that almost 10% have positive DATs without any evidence of hemolysis. 3,5,[18][19][20] However, in most instances, the DATs were very weakly reactive, 1+ or less, and complement alone was detected on the red cells.…”

Section: Discussionmentioning

confidence: 96%

Warm-reactive Autoantibodies in Pediatric Patients

Blackall¹

2007

Journal of Pediatric Hematology/Oncology

View full text Add to dashboard Cite

The significance of warm-reactive autoantibodies in pediatric patients has not been a subject of thorough evaluation. This study was undertaken to correlate the clinical and serologic features of these antibodies to identify predictors of clinical significance. Forty-two consecutive patients with serologically detectable warm-reactive autoantibodies were studied. These patients (21 male, 21 female) had a mean age of 9 years (range: 2 mo to 21 y). Primary diagnoses included autoimmune disorders (14), sickle cell disease (14), viral infection (4), idiopathic autoimmune hemolytic anemia (2), leukemia (2), and other diseases (6). Autoimmune hemolysis, as determined by clinical and laboratory findings, was documented in 24 patients (57%). Serologic studies revealed that all patients demonstrated IgG on their red cells [Direct Antiglobulin Test (DAT) reactivity range: microscopic to 3+]; 17 (40%) also demonstrated complement (DAT reactivity range: microscopic to 2+). There was a correlation between the strength of the DAT for IgG and the presence of complement on the red cells, with both being important predictors of hemolysis. These findings may be useful in predicting the clinical significance of warm-reactive autoantibodies in pediatric patients and allow for more efficient and effective follow-up care.

show abstract

Section: Discussionmentioning

confidence: 96%

Warm-reactive Autoantibodies in Pediatric Patients

Blackall¹

2007

Journal of Pediatric Hematology/Oncology

View full text Add to dashboard Cite

show abstract

“…Small amounts of C3 fragments have previously been detected on E from healthy individuals [4,[9][10][11], while increased amounts of C3d,g have been demonstrated on E from patients with inflammatory diseases, such as SLE or rheumatoid arthritis (RA) [4,[9][10][11]. We observed that all subpopuiations of SLE blood leucocytes, as well as E, carry raised levels of in v/vo-deposited C3d,g, but not C3b or iC3b fragments, compared with normal individuals.…”

Section: Discussionmentioning

confidence: 99%

“…The erythrocytes (E) of many patients with SLE display a relative deficiency of complement receptor (CRl) [1][2][3][4][5][6][7][8] as well as increased deposition of the C3-split product, C3d,g, on the E-membrane [9][10][11][12]. Both inherited [3,7] and acquired [1,4,6] factors have been reported to contribute to the deficiency of E-CRl in SLE patients.…”

Section: Introductionmentioning

confidence: 99%

Complement receptor expression and activation of the complement cascade on B lymphocytes from patients with systemic lupus erythematosus (SLE)

Marquart

Svendsen

Rasmussen

et al. 1995

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYIt has previously been reported that the expression of the complement receptors, CRl on erythrocytes and blood leucocytes and CR2 on B cells, is reduced in patients with SLE, and that the reduced expression of CRl on erythrocytes is related to disease activity. We have earlier demonstrated that normal B cells are capable of activating the alternative pathway (AP) of complement in a CR2-dependent fashion. In this study we have investigated whether disturbances in this activity may be related to the altered phenotype of SLE B cells. Flow cytometry was used to measure expression of complement receptors and regulatory proteins on B cells from SLE patients, as well as the deposition of C3 fragments occurring in vivo or after in vitro AP activation. We have confirmed, for a proportion of the patients studied, reduced expression of CRl and CR2 on B cells, and shown a consistency between low CR2 expression and reduced in vitro AP activation in the presence of homologous, normal serum. In addition, the B cells, like erythrocytes, bear raised levels of in v/vo-deposited C3dg, but not C3b fragments, compared with normal B cells. The erythrocytes from SLE patients were unable to inhibit in vitro AP activation by B cells in homologous serum. Finally, we demonstrated an inverse relationship between SLE disease activity index (SLEDAI) and the expression of complement receptor 2 (CR2) on SLE B cells. Thus, determination of CR2 on B cells may emerge as an additional laboratory tool in the assessment of SLE activity.

show abstract

“…If it deposits on healthy self-tissue or ticks over in the fluid phase, host regulatory proteins immediately inactivate it. Evidence of this AP turnover is that a red blood cell contains on average several hundred C3d fragments covalently bound to its membrane (5). In addition, a small percentage of serum albumin migrates at approximately 100 kDa, not 55, because of the attachment to it of a C3d fragment (6).…”

Section: Complement Pathwaysmentioning

confidence: 99%

Complement Dysregulation and Disease: Insights from Contemporary Genetics

Liszewski

Java

Schramm³

et al. 2017

Annu. Rev. Pathol. Mech. Dis.

View full text Add to dashboard Cite

The vertebrate complement system consists of sequentially interacting proteins that provide for a rapid and powerful host defense. Nearly 60 proteins comprise three activation pathways (classical, alternative, and lectin) and a terminal cytolytic pathway common to all. Attesting to its potency, nearly half of the system’s components are engaged in its regulation. An emerging theme over the past decade is that variations in these inhibitors predispose to two scourges of modern humans. One, occurring most often in childhood, is a rare but deadly thrombomicroangiopathy called atypical hemolytic uremic syndrome. The other, age-related macular degeneration, is the most common form of blindness in the elderly. Their seemingly unrelated clinical presentations and pathologies share the common theme of overactivity of the complement system’s alternative pathway. This review summarizes insights gained from contemporary genetics for understanding how dysregulation of this powerful innate immune system leads to these human diseases.

show abstract

Quantitation of Red Blood Cell‐bound C3d in Normal Subjects and Random Hospitalized Patients

Cited by 49 publications

References 9 publications

Warm-reactive Autoantibodies in Pediatric Patients

Warm-reactive Autoantibodies in Pediatric Patients

Complement receptor expression and activation of the complement cascade on B lymphocytes from patients with systemic lupus erythematosus (SLE)

Complement Dysregulation and Disease: Insights from Contemporary Genetics

Contact Info

Product

Resources

About