Quantitation of PGE9509924, a novel, nonfluorinated quinolone, in rat plasma using liquid chromatography electrospray-tandem mass spectrometry following solid-phase extraction sample clean-up in a 96-well format

Zoutendam, Paul H.; Gavin, Martin J.; Martín, Mabel; Dirr, Mary Kay

doi:10.1016/s0731-7085(03)00362-5

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…robotic liquid handling systems, incubators, vacuum manifolds, and so on, to handle plate manipulation [109,122]. Although 96-well plate formats for filtration [126][127][128][129], solid phase extraction [130][131][132][133][134] and liquid-liquid extraction [135][136][137][138][139] have all been used in automated sample preparation, there is a trend to bring the microplate into the nanoplate range (larger than 384). Filter plates can be used to collect, store frozen and then filter plasma samples prior to HPLC-MS/MS analysis and help to avoid thrombin clots during the freeze/thaw process [126].…”

Section: Microplate Techniquesmentioning

confidence: 99%

Increasing Speed and Throughput When Using HPLC-MS/MS Systems forDrug Metabolism and Pharmacokinetic Screening

Hsieh¹,

Korfmacher²

2006

CDM

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Both combinatorial chemistry and parallel synthesis provide a valuable means for the production of large numbers of compounds with diverse molecular architectures that become available for various drug discovery experiments. In both the lead optimization and lead selection stages, one requirement that is common for many processes is the need for bioanalytical support. This review summarizes current high throughput strategies and efficient methodologies that are employed for drug metabolism and pharmacokinetic (DMPK) screens for a series of drug discovery compounds. For these types of assays, high performance liquid chromatography coupled to a tandem mass spectrometer (HPLC-MS/MS) has now become the technique of choice. The major high throughput strategies including sample reduction and cassette dosing are discussed. The methods for increasing the speed of HPLC-MS/MS-based analyses, such as fast chromatography, direct sample injection, parallel technologies and combined ionization interfaces are also presented in this review. In addition, the special challenges when performing HPLC-MS/MS bioanalysis, such as the choice of ionization sources, matrix ionization suppression and the potential for endogenous interferences, are addressed.

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Section: Microplate Techniquesmentioning

confidence: 99%

Increasing Speed and Throughput When Using HPLC-MS/MS Systems forDrug Metabolism and Pharmacokinetic Screening

Hsieh¹,

Korfmacher²

2006

CDM

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“…Many methods, including HPLC/UV, HPLC/FLD and HPLC/MS/MS have been reported for the determination of quinolones in biofluids (Huang et al ., ; Raju et al ., ; Roy et al ., ; Jin et al ., ; Fang et al ., ; Pranger et al ., ; Lin et al ., ; Bian et al ., ; Conte et al ., ; Al‐Dgither et al ., ; Zoutendam et al ., ; Vishwanathan et al, ; Zheng et al ., ; Bai et al ., ; Ma et al ., ; Liu et al ., ; Wang et al ., , ). However, only a few methods published were to determine caderofloxacin (Bai et al ., ; Liu et al ., ; Wang et al ., ).…”

Section: Introductionmentioning

confidence: 98%

A validated HPLC‐MS/MS method for the quantification of caderofloxacin in human plasma and its application to a clinical pharmacokinetic study

Zhang

Zhai

Duan

et al. 2015

Biomedical Chromatography

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A simple, selective and rapid HPLC-MS/MS method was developed and validated for the determination of caderofloxacin in human plasma. Sparfloxacin was used as the internal standard (IS). After precipitation with methanol and dilution with the mobile phase, the samples were injected into the HPLC-MS/MS system. The chromatographic separation was performed on a Zorbax XDB Eclipse C18 column (150 × 4.6 mm, 5 µm) with a mobile phase of ammonium acetate buffer (20 mm, pH 3.0)-methanol, 45:55 (v/v). The MS/MS analysis was done in positive mode. The multiple reaction monitoring transitions monitored were m/z 412.3 → 297.1 for caderofloxacin and m/z 393.2 → 292.2 for the IS. The calibration curve was linear over the range of 50.0-8000 ng/mL with an aliquot of 100 μL plasma. The precision of the assay was 2.0-9.4 and 6.6-11.5% for the intra- and inter-run variability, respectively. The intra- and inter-run accuracy (relative error) was 4.4-10.0 and -1.2-4.0%. The total run time was 3.5 min. The assay was fully validated in accordance with the US Food and Drug Administration guidance. It was successfully applied to a pharmacokinetic study of caderofloxacin in healthy Chinese volunteers.

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“…Pruvost). or blood have been previously described. These LC systems with UV and fluorescence detection [5][6][7] reach a lower limit of quantification (LLOQ) in the range 25-100 and 5 ng/mL for the most recently developed [8,9] and electrochemical detection [10], while with mass spectrometry (MS) and MS/MS detection the LLOQ ranges between 0.1 and 10 ng/mL [11][12][13][14]. The contribution of LC-MS/MS to the bioanalysis of xenobiotics and particularly of other HIV reverse transcriptase and protease inhibitors in plasma and in intracellular media is now well established [15][16][17][18], in terms of fast development, runtime reduction, sensitivity and specificity enhancement, which are major parameters in multidrug therapy.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and specific LC–ESI–MS/MS method for the determination of a styrylquinoline, BA011FZ041, a potent HIV anti-integrase agent, in rat plasma

Pruvost

Levi²,

Zouhiri³

et al. 2007

Journal of Chromatography B

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Quantitation of PGE9509924, a novel, nonfluorinated quinolone, in rat plasma using liquid chromatography electrospray-tandem mass spectrometry following solid-phase extraction sample clean-up in a 96-well format

Cited by 9 publications

References 14 publications

Increasing Speed and Throughput When Using HPLC-MS/MS Systems forDrug Metabolism and Pharmacokinetic Screening

Increasing Speed and Throughput When Using HPLC-MS/MS Systems forDrug Metabolism and Pharmacokinetic Screening

A validated HPLC‐MS/MS method for the quantification of caderofloxacin in human plasma and its application to a clinical pharmacokinetic study

Sensitive and specific LC–ESI–MS/MS method for the determination of a styrylquinoline, BA011FZ041, a potent HIV anti-integrase agent, in rat plasma

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