Quantitation of natural killer cell precursors in man

Gharehbaghian, Ahmad; Haque, K.M.G.; Truman, Carol; Newman, John H.; Bradley, Benjamin A.

doi:10.1016/s0022-1759(01)00534-8

Cited by 7 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, others showed that non-anti-coagulated salvaged blood conferred no change in blood levels of NK cells, albeit identified by the CD56 + CD16 + phenotype using flow cytometry and suppression of immune status [26][27][28]. Our NKp cell subset would have been negative for CD16, and only measurable by limiting dilutions analysis at levels well below detection by routine flow cytometry [29][30][31].…”

Section: Introductionmentioning

confidence: 70%

See 1 more Smart Citation

Post-traumatic immunosuppression is reversed by anti-coagulated salvaged blood transfusion: deductions from studying immune status after knee arthroplasty

Islam

Whitehouse

Mehendale

et al. 2014

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryMajor trauma increases vulnerability to systemic infections due to poorly defined immunosuppressive mechanisms. It confers no evolutionary advantage. Our objective was to develop better biomarkers of post-traumatic immunosuppression (PTI) and to extend our observation that PTI was reversed by anti-coagulated salvaged blood transfusion, in the knowledge that others have shown that non-anti-coagulated (fibrinolysed) salvaged blood was immunosuppressive. A prospective non-randomized cohort study of patients undergoing primary total knee arthroplasty included 25 who received salvaged blood transfusions collected post-operatively into acid-citrate-dextrose anti-coagulant (ASBT cohort), and 18 non-transfused patients (NSBT cohort). Biomarkers of sterile trauma included haematological values, damage-associated molecular patterns (DAMPs), cytokines and chemokines. Salvaged blood was analysed within 1 and 6 h after commencing collection. Biomarkers were expressed as fold-changes over preoperative values. Certain biomarkers of sterile trauma were common to all 43 patients, including supranormal levels of: interleukin (IL)-6, IL-1-receptor-antagonist, IL-8, heat shock protein-70 and calgranulin-S100-A8/9. Other proinflammatory biomarkers which were subnormal in NSBT became supranormal in ASBT patients, including IL-1β, IL-2, IL-17A, interferon (IFN)-γ, tumour necrosis factor (TNF)-α and annexin-A2. Furthermore, ASBT exhibited subnormal levels of anti-inflammatory biomarkers: IL-4, IL-5, IL-10 and IL-13. Salvaged blood analyses revealed sustained high levels of IL-9, IL-10 and certain DAMPs, including calgranulin-S100-A8/9, alpha-defensin and heat shock proteins 27, 60 and 70. Active synthesis during salvaged blood collection yielded increasingly elevated levels of annexin-A2, IL-1β, Il-1-receptor-antagonist, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IFN-γ, TNF-α, transforming growth factor (TGF)-β1, monocyte chemotactic protein-1 and macrophage inflammatory protein-1α. Elevated levels of high-mobility group-box protein-1 decreased. In conclusion, we demonstrated that anti-coagulated salvaged blood reversed PTI, and was attributed to immune stimulants generated during salvaged blood collection.

show abstract

Section: Introductionmentioning

confidence: 70%

“…Our NKp cell subset would have been negative for CD16, and only measurable by limiting dilutions analysis at levels well below detection by routine flow cytometry [29][30][31].…”

Section: Cd16mentioning

confidence: 99%

Post-traumatic immunosuppression is reversed by anti-coagulated salvaged blood transfusion: deductions from studying immune status after knee arthroplasty

Islam

Whitehouse

Mehendale

et al. 2014

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…Several approaches (24-33) have been described to assess immune lysis, including the classical [ 51 Cr]-release assay (26,27), the europium (Eu3+)-release assay (28)(29)(30), BLT esterase activity (31), b-galactosidase release (32), FACS assays (33), and video microscopy (34,35). Particularly relevant to the current study, a microfabricated device has been described (36) and is commercially available (xCELLigence; http://www.aceabio.com/case_info.aspx?id=249), enabling the dynamic, real-time, label-free detection of NK cell lysis by measurement of electric impedance in a microtiter format.…”

Section: Discussionmentioning

confidence: 99%

Lysis-on-Chip of Single Target Cells following Forced Interaction with CTLs or NK Cells on a Dielectrophoresis-Based Array

Abonnenc¹,

Borgatti

Fabbri

et al. 2013

The Journal of Immunology

View full text Add to dashboard Cite

Guiding the interaction of single cells acting as partners in heterotypic interactions (e.g., effectors and targets of immune lysis) and monitoring the outcome of these interactions are regarded as crucial biomedical achievements. In this study, taking advantage of a dielectrophoresis (DEP)-based Laboratory-on-a-chip platform (the DEPArray), we show that it is possible to generate closed DEP cages entrapping CTLs and NK cells as either single cells or clusters; reversibly immobilize a single virus-presenting or tumor cell within the chip at a selected position; move cages and their content to predetermined spatial coordinates by software-guided routing; force a cytotoxic effector to physically interact with a putative target within a secluded area by merging their respective cages; generate cages containing effector and target cells at predetermined E:T ratios; accurately assess cytotoxicity by real-time quantitation of the release kinetics of the fluorescent dye calcein from target cells (>50 lytic events may be tested simultaneously); estimate end points of calcein release within 16 min of initial E:T cell contact; simultaneously deliver Ab-based phenotyping and on-chip lysis assessment; and identify lytic and nonlytic E:T combinations and discriminate nonlytic effector phenotypes from target refractoriness to immune lysis. The proof of principle is provided that DEPArray technology, previously used to levitate and move single cells, can be used to identify highly lytic antiviral CTLs and tumor cells that are particularly refractory to NK cell lysis. These findings are of primary interest in targeted immunotherapy.

show abstract

“…NK cell development and maturation primarily occur in the bone marrow, where common lymphoid progenitors (CLPs) differentiate into NK precursors (NKPs), immature NK cells, and finally mature NK cells [ 12 ]. Notably, recombinant interleukin (rIL)-15 plays a crucial role in NK cell development from hematopoietic stem cells [ 16 ]. In humans, CD122 expression on NKPs is crucial for NK cell lineage commitment, and CD56 expression is a final step in the differentiation of NKPs into NK cells [ 17 ].…”

Section: Nk Cells: Biology Receptors and Functionsmentioning

confidence: 99%

Harnessing natural killer cells for refractory/relapsed non-Hodgkin lymphoma: biological roles, clinical trials, and future prospective

Bakhtiyaridovvombaygi,

Yazdanparast,

Kheyrandish

et al. 2024

Biomark Res

View full text Add to dashboard Cite

Non-Hodgkin lymphomas (NHLs) are heterogeneous and are among the most common hematological malignancies worldwide. Despite the advances in the treatment of patients with NHLs, relapse or resistance to treatment is anticipated in several patients. Therefore, novel therapeutic approaches are needed. Recently, natural killer (NK) cell-based immunotherapy alone or in combination with monoclonal antibodies, chimeric antigen receptors, or bispecific killer engagers have been applied in many investigations for NHL treatment. The functional defects of NK cells and the ability of cancerous cells to escape NK cell-mediated cytotoxicity within the tumor microenvironment of NHLs, as well as the beneficial results from previous studies in the context of NK cell-based immunotherapy in NHLs, direct our attention to this therapeutic strategy. This review aims to summarize clinical studies focusing on the applications of NK cells in the immunotherapy of patients with NHL.

show abstract

Quantitation of natural killer cell precursors in man

Cited by 7 publications

References 38 publications

Post-traumatic immunosuppression is reversed by anti-coagulated salvaged blood transfusion: deductions from studying immune status after knee arthroplasty

Post-traumatic immunosuppression is reversed by anti-coagulated salvaged blood transfusion: deductions from studying immune status after knee arthroplasty

Lysis-on-Chip of Single Target Cells following Forced Interaction with CTLs or NK Cells on a Dielectrophoresis-Based Array

Harnessing natural killer cells for refractory/relapsed non-Hodgkin lymphoma: biological roles, clinical trials, and future prospective

Contact Info

Product

Resources

About