Quantitation of nasal development in the early prenatal period using geometric morphometrics and MRI: a new insight into the critical period of Binder phenotype

Katsube, Motoki; Yamada, Shigehito; Miyazaki, Reina; Yamaguchi, Yasushi; Makishima, Hideki; Takakuwa, Tetsuya; Yamamoto, Akira; Fujii, Yosuke; Morimoto, Naoki; Ito, Tsuyoshi; Imai, Hirohiko; Suzuki, Shigehiko

doi:10.1002/pd.5106

Cited by 12 publications

(18 citation statements)

References 45 publications

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Section: Methodsmentioning

confidence: 99%

“…The Congenital Anomaly Research Center at Kyoto University, Japan has maintained a large collection of human conceptuses since 1961, collected under the Maternity Protection Law of Japan. 22,23 Specimens have been fixed in formalin upon collection, preserving soft and hard tissues. Conceptuses without any distinct congenital anomalies and artificial deformities of the face were selected for the present study (n = 21, crown-rump length 98-198 mm, ~15-20 weeks of gestation).…”

Section: Specimensmentioning

confidence: 99%

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Analysis of facial skeletal asymmetry during foetal development using μCT imaging

Katsube

Rolfe

Bortolussi

et al. 2019

Orthod Craniofacial Res

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Structured Abstract Objectives Asymmetry has been noted in the human craniofacial region in several pathological conditional and growth abnormalities, often with a directional predilection. Physiological asymmetry has also been reported in normal adults and adolescents, with certain regions of the cranioskeleton, such as the mandible, displaying prevalent asymmetry. However, the timing at which such asymmetries arise has not been evaluated. The objectives of this study were to assess the degree of asymmetry in facial bones during the foetal stages of human development. Material and Methods Twenty‐one preserved conceptuses from the Congenital Anomaly Research Center at Kyoto University, between ages 15 and 20 weeks of gestation, were studied using high‐resolution μCT imaging. Asymmetry analysis was performed on digitally segmented facial bone pairs, using geometric morphometric (GM) approaches as well as adapted deformation‐based asymmetry (DBA) methods. Results GM analysis revealed that the developing facial bones display statistically significant fluctuating and directional asymmetry. DBA methods suggest that the magnitude of asymmetry in facial bones is low and does not appear to be correlated to the estimate of overall size of conceptus. Additionally, the patterns of asymmetry are highly variable between individual specimens. Conclusions The developing foetal facial skeleton displays variable patterns of low magnitude asymmetry. GM and DBA methods offer unique advantages to assess facial asymmetry quantitatively and qualitatively.

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Section: Methodsmentioning

confidence: 99%

Section: Specimensmentioning

confidence: 99%

Analysis of facial skeletal asymmetry during foetal development using μCT imaging

Katsube

Rolfe

Bortolussi

et al. 2019

Orthod Craniofacial Res

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“…Accordingly, the shape of the midfacial skeleton is a complicated 3-dimensional structure. The shape of the midfacial skeleton has been reported to change drastically; it is presumably established during the early fetal period (Burdi, 1969; Johnston, 1974; Diewert, 1985; Katsube et al, 2017). However, the growth trend of the midfacial skeleton during this period has been poorly documented.…”

Section: Introductionmentioning

confidence: 99%

Critical Growth Processes for the Midfacial Morphogenesis in the Early Prenatal Period

Katsube

Yamada

Yamaguchi

et al. 2019

The Cleft Palate-Craniofacial Journal

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Background: Congenital midfacial hypoplasia often requires intensive treatments and is a typical condition for the Binder phenotype and syndromic craniosynostosis. The growth trait of the midfacial skeleton during the early fetal period has been assumed to be critical for such an anomaly. However, previous embryological studies using 2-dimensional analyses and specimens during the late fetal period have not been sufficient to reveal it. Objective: To understand the morphogenesis of the midfacial skeleton in the early fetal period via 3-dimensional quantification of the growth trait and investigation of the developmental association between the growth centers and midface. Methods: Magnetic resonance images were obtained from 60 human fetuses during the early fetal period. Three-dimensional shape changes in the craniofacial skeleton along growth were quantified and visualized using geometric morphometrics. Subsequently, the degree of development was computed. Furthermore, the developmental association between the growth centers and the midfacial skeleton was statistically investigated and visualized. Results: The zygoma expanded drastically in the anterolateral dimension, and the lateral part of the maxilla developed forward until approximately 13 weeks of gestation. The growth centers such as the nasal septum and anterior portion of the sphenoid were highly associated with the forward growth of the midfacial skeleton (RV = 0.589; P < .001). Conclusions: The development of the midface, especially of the zygoma, before 13 weeks of gestation played an essential role in the midfacial development. Moreover, the growth centers had a strong association with midfacial forward growth before birth.

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“…Specific organs and tissues, such as the heart and bronchial tubes, can also be extracted using 3D images . Similar 3D imaging of development has been performed using magnetic resonance imaging (MRI) for the brain (Yamaguchi et al 2017) and nasal region (Katsube et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Blechschmidt Collection: Revisiting specimens from a historical collection of serially sectioned human embryos and fetuses using modern imaging techniques

Miyazaki

Makishima

Männer

et al. 2018

Congenital Anomalies

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Along with the Carnegie Collection in the United States and the Kyoto Collection in Japan, the Blechschmidt Collection (Georg-August-University of Göttingen, Germany) is a major historical human embryo and fetus collection. These collections are of enormous value to human embryology; however, due to the nature of the historical histological specimens, some stains are fading in color, and some glass slides are deteriorating over time. To protect these specimens against such degradation and ensure their future usefulness, we tried to apply modern image scanning and computational reconstruction. Samples of histological specimens of the Blechschmidt Collection were digitized into images using commercial flatbed scanners with a resolution of 4800 pixels per inch. Two specimens were reconstructed into three-dimensional (3D) images by using modern techniques to vertically stack two-dimensional images of the slices into 3D blocks. The larger specimen of crown-rump length (CRL) 64.0 mm, a series of very large histological sections in human embryology, was reconstructed clearly, with its central nervous system segmented before stacking. The smaller specimen of CRL 17.5 mm was also reconstructed into 3D images. The outer surface of the embryo was intact, and its development was classified according to the widely used Carnegie stages (CSs). The CS of the specimen was identified as the later half of CS 20. The invaluable Blechschmidt Collection can be revisited for further research with modern techniques such as digital image scanning and computational 3D reconstruction.

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Quantitation of nasal development in the early prenatal period using geometric morphometrics and MRI: a new insight into the critical period of Binder phenotype

Cited by 12 publications

References 45 publications

Analysis of facial skeletal asymmetry during foetal development using μCT imaging

Analysis of facial skeletal asymmetry during foetal development using μCT imaging

Critical Growth Processes for the Midfacial Morphogenesis in the Early Prenatal Period

Blechschmidt Collection: Revisiting specimens from a historical collection of serially sectioned human embryos and fetuses using modern imaging techniques

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