“…-Cannot distinguish between intact and defective provirus so overestimates the reservoir -Quantification relative to a standard so prone to bias -Highly specific and prone to error from primer/template mismatches Kostrikis et al, 2002Beloukas et al, 2009van der Sluis et al, 2013Munir et al, 2013Casabianca et al, 2014Rouzioux et al, 2014Vandergeeten et al, 2014 Brussel et al, 2005Yu et al, 2008Liszewski et al, 2009Brady et al, 2013Agosto et al, 2007De Spiegelaere et al, 2014Vandergeeten et al, 2014Lada et al, 2018 Digital Henrich et al, 2012Strain et al, 2013Malatinkova et al, 2015Henrich et al, 2017Lada et al, 2018 Cell associated RNA Measures all or different forms of cell associated RNA with the rationale that it is more likely to measure replication competent provirus than defective -More sensitivity for replication competent provirus -Cannot distinguish transcripts that arise from replication competent cells and defective cells Archin et al, 2012Pasternak et al, 2012Shan et al, 2013Cillo et al, 2014Yucha et al, 2017Massanella et al, 2018Yukl et al, 2018 TILDA Measures multiply spliced tat/rev transcripts following stimulation of CD4 T cells plated in limiting dilution -Higher sensitivity for replication competent provirus -Faster, cheaper and less resources needed than VOA Measured transcripts may arise from defective proviral genomes Procopio et al, 2015Frank et al, 2019Bertoldi et al, 2020 ISH and flow cytometry…”