Quantitation of<i>Helicobacter pylori</i>in dental plaque samples by competitive polymerase chain reaction

Song, Qunsheng; Haller, B; Dobrindt, Ulrich; Wichelhaus, Andrea; Adler, Guido; Bode, Günter

doi:10.1136/jcp.53.3.218

Cited by 38 publications

(47 citation statements)

References 25 publications

(17 reference statements)

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“…While some studies have shown higher frequency of H. pylori in dental plaque (15), this paper has identified the bacteria in similar proportions in dental plaque and in saliva samples. However, according to the literature, the results depend largely on the detection method chosen (11). In patients with positive biopsies for H. pylori, the difference between sexes was not statistically significant.…”

Section: Among the Biology Molecular Techniques Polymerasementioning

confidence: 90%

“…Chain Reaction (PCR) has demonstrated to be specific and sensible, that is why it is considered the method of choice to detect DNA from H. pylori in the oral cavity and for clinical control and follow-up of the patients (1,7,11). It has been used also in a wide range of ages, in which the diagnostic criteria by endoscopic examination were imprecise (12).…”

Section: Among the Biology Molecular Techniques Polymerasementioning

confidence: 99%

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Molecular detection of Helicobacter pylori in oral samples from patients suffering digestive pathologies

Medina¹,

Medina²,

Martín³

et al. 2009

Med Oral

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Objective: to determine the simultaneous presence of H. pylori in both the oral cavity and gastric mucosal in patients suffering digestive pathologies and to establish the possible association between the presence of H. pylori in the oral cavity and the gingivoperiodontal pathology. Study design: Patients with gastric symptoms (case group) and asymptomatic patients (control group) seen at the Gastroenterology Department of Dr. Julio C. Perrando Hospital (Resistencia, Argentina) were selected. Dental plaque and saliva samples from both groups were obtained. In the case group, gastric biopsy samples were also taken. H. pylori was detected in gastric biopsies by histological stains, and Polymerase Chain Reaction (PCR) was carried out on the oral samples. Results: Among the 98 patients (43 cases and 55 controls), 196 oral samples (saliva 98, dental plaque 98) and 43 gastric biopsias were obtained. H. pylori was detected in oral samples in 18/98 patients, in gastric biopsies in 38/43 patients, and in both samples in 15/43 patients. Conclusions: The presence of H. pylori in the oral cavity of patients suffering digestive pathologies is more frequent in those patients harbouring a gingivoperiodontal disease, and this fact could represent an obstacle for the eradication of the bacterium. At the same time, it could constitute a risk factor for gastrointestinal reinfection after treatment.

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Section: Among the Biology Molecular Techniques Polymerasementioning

confidence: 90%

Section: Among the Biology Molecular Techniques Polymerasementioning

confidence: 99%

Molecular detection of Helicobacter pylori in oral samples from patients suffering digestive pathologies

Medina¹,

Medina²,

Martín³

et al. 2009

Med Oral

View full text Add to dashboard Cite

show abstract

“…This study investigated only adult patients. As H. pylori infection is mainly acquired in childhood [33], and the number of H. pylori in the mouths of adults is very low, this does not seem to be a signi®cant risk for hosts themselves [34,35]. According to a recent study, it seems likely that an infected mother may play a key role in transmission of H. pylori to her child, possibly via saliva [36].…”

Section: Discussionmentioning

confidence: 99%

Characteristic distribution pattern of Helicobacter pylori in dental plaque and saliva detected with nested PCR

Song¹,

Lange

Spahr

et al. 2000

Journal of Medical Microbiology

154

142

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The precise mode of transmission and the natural reservoir for Helicobacter pylori are unknown. PCR assays have proved to be highly sensitive and speci®c and are regarded as the method of choice for detecting H. pylori DNA in the oral cavity. The aim of this study was to investigate the prevalence and distribution of H. pylori in the oral cavity. Forty-two patients undergoing gastroscopy were investigated for the presence of H. pylori in dental plaque and saliva by nested PCR, and in the stomach by the 13 C-urea breath test. Samples tested comprised dental plaque from molars, premolars and incisors and saliva. Two sets of primers homologous to the 860-bp fragment of H. pylori DNA, which have been shown previously to be highly sensitive and speci®c, were used for nested PCR. Eleven patients (26.2%) were infected with H. pylori in the stomach. H. pylori DNA was identi®ed in dental plaque samples from 41 patients (97%) and in 23 saliva samples (55%). The prevalence in dental plaque from molars, premolars and incisors was 82%, 64% and 59%, with an odds ratio of 3.18, 1.24 and 1 (reference), respectively. In conclusion, H. pylori was present in the oral cavity of 97% of tested patients, with a characteristic distribution that was independent of the infection status of the stomach. Thus H. pylori may belong to the normal oral micro¯ora.

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“…There is disagreement among scientists regarding the prevalence of Helicobacter pylori in the oral cavity of humans. Some consider the flora to be normal and believe it maintains a symbiotic relationship with the host flora while others believe it enters the oral cavity during a gastroesophageal reflux (Savoldi et al 1998, Checchi et al 2000, Song et al 2000.…”

Section: Discussionmentioning

confidence: 99%

Detection of Helicobacter spp. in the saliva of dogs with gastritis

Jankowski¹,

Spużak²,

Kubiak³

et al. 2016

Polish Journal of Veterinary Sciences

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The aim of this study was to identify the species and determine the prevalence of gastric Helicobacter in the saliva of dogs with gastritis. The study was carried out on 30 dogs of different breeds, genders and ages, which were diagnosed with gastritis. The nested-PCR method was used to detect Helicobacter spp. in saliva. Helicobacter bacteria were found in the saliva samples of 23 (76.6%) dogs. Helicobacter heilmannii was the most commonly detected species of gastric Helicobacter spp. in canine saliva, and was found in 22 (73.3%) cases. The results indicate that gastric Helicobacter spp. occurs relatively frequently in dogs with gastritis. Moreover, the saliva of dogs with gastritis may be a source of Helicobacter spp. infection for humans and other animals. However, further studies are needed to confirm this finding as the PCR method does not distinguish active from inactive infections.

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Quantitation ofHelicobacter pyloriin dental plaque samples by competitive polymerase chain reaction

Cited by 38 publications

References 25 publications

Molecular detection of Helicobacter pylori in oral samples from patients suffering digestive pathologies

Molecular detection of Helicobacter pylori in oral samples from patients suffering digestive pathologies

Characteristic distribution pattern of Helicobacter pylori in dental plaque and saliva detected with nested PCR

Detection of Helicobacter spp. in the saliva of dogs with gastritis

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