Quantitation of human cytomegalovirus genomes in the brain of AIDS patients

Kühn, Joachim; Wendland, Thomas; Eggers, Hans J.; Lorentzen, Eva; Wieland, Ulrike; Eing, Bodo R.; Kiessling, Marika; Gass, Peter

doi:10.1002/jmv.1890470114

Cited by 25 publications

(9 citation statements)

References 45 publications

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“…For preparation of each case, the sectioning blade was replaced and the cutting surface was cleansed with xylene and ethanol. From each sample, 100-250 ng of DNA was amplified by nested PCR using internal and external primers specific for HCMV glycoprotein B (UL55) gene as described [39]. Samples were considered positive when a band of 140 bp size could be visualized on agarose gel with ethidium bromide.…”

Section: Methodsmentioning

confidence: 99%

Detection of human cytomegalovirus in normal and neoplastic breast epithelium

et al. 2010

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IntroductionHuman cytomegalovirus (HCMV) establishes a persistent life-long infection, and can cause severe pathology in the fetus and the immunocompromised host[1]. Breast milk is the primary route of transmission in humans worldwide, and breast epithelium is thus a likely site of persistent infection and/or reactivation, though this phenomenon has not previously been demonstrated. Increasing evidence indicates HCMV infection can modulate signaling pathways associated with oncogenesis. We hypothesized that persistent HCMV infection occurs in normal adult breast epithelium and that persistent viral expression might be associated with normal and neoplastic ductal epithelium.MethodsSurgical biopsy specimens of normal breast (n = 38) breast carcinoma (n = 39) and paired normal breast from breast cancer patients (n = 21) were obtained. Specimens were evaluated by immunohistochemistry, in situ hybridization, PCR and DNA sequencing for evidence of HCMV antigens and nucleic acids.ResultsWe detected HCMV expression specifically in glandular epithelium in 17/27 (63%) of normal adult breast cases evaluated. In contrast, HCMV expression was evident in the neoplastic epithelium of 31/32 (97%) patients with ductal carcinoma in situ (DCIS) and infiltrating ductal carcinoma (IDC) cases evaluated (p = 0.0009).ConclusionsThese findings are the first to demonstrate that persistent HCMV infection occurs in breast epithelium in a significant percentage of normal adult females. HCMV expression was also evident in neoplastic breast epithelium in a high percentage of normal and neoplastic breast tissues obtained from breast cancer patients, raising the possibility that viral infection may be involved in the neoplastic process.

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Section: Methodsmentioning

confidence: 99%

Detection of human cytomegalovirus in normal and neoplastic breast epithelium

et al. 2010

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“…The sequences of the inside primer pair were as follows: 5'-GCCCGCCGCGGCAGCACCTGGCT-3' and 5'-GTAAACCACATCACCCGTGGA-3'. 4 A regular PCR cycling protocol was used: 95°C for 2 minutes, 1 cycle; 92°C for 20 seconds, 55°C for 30 seconds, and 70°C for 30 seconds, 30 cycles. At the end of amplification, 10 µL of the PCR products was analyzed on 1.2% agarose gels.…”

Section: Nested Pcr Amplificationmentioning

confidence: 99%

Is Cytomegalovirus Associated With Human Colorectal Tumorigenesis?

Akintola-Ogunremi

Luo

et al. 2005

Am J Clin Pathol

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Despite the rapid advance in the understanding of molecular pathways underlying human colorectal tumorigenesis, the causes that initiate dysregulation of the pathways remain largely unknown. Human cytomegalovirus (CMV) has been implicated as a potential etiopathogenetic agent. To further investigate whether CMV participates in human colorectal tumorigenesis, we examined 23 colorectal hyperplastic polyps, 65 colorectal adenomas, and 51 colorectal adenocarcinomas by immunohistochemical analysis using 2 antibody mixtures that recognize CMV immediate early, early, and delayed gene products. The results show that while typical nuclear staining (with or without cytoplasmic positivity) was observed in control cases of CMV colitis, no nuclear positivity was detected in any cases studied. Focal and weak cytoplasmic staining was noted in a subset of cases, particularly when a higher antibody concentration was used. This staining was believed to be nonspecific, however, because it also was observed in normal-appearing colonic mucosa. In addition, polymerase chain reaction failed to detect the presence of CMV DNA in 24 selected cases showing nonspecific cytoplasmic immunostaining. These observations demonstrate an absence of CMV proteins and DNA in human colorectal adenocarcinomas and their precursor lesions.

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“…External and internal oligonucleotide primers specific for CMV glycoprotein B gene were utilized. 20 The external primers used were 5 0 -TCCAACACC CACAGTACCCGT-3 0 and 5 0 -CGGAAACGATGGTG TAGTTCG-3 0 and the internal primers used were 5 0 -TGACGGTCAAGGATCAGTGGC-3 0 and 5 0 -GTAAA CCACATCACCCGTGGA-3 0 . The expected amplification product size was 146 bp.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Lack of association of cytomegalovirus with human brain tumors

et al. 2005

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Cytomegalovirus (CMV) is thought to possess oncogenic properties and has been linked with a number of human malignancies. CMV infection was recently described in association with malignant gliomas. The intent of the present study was to further investigate the reported association between CMV and malignant gliomas. Tissue from 22 brain tumors of various histologic types and grades, four normal brains, six breast carcinomas, six colon carcinomas, six lung carcinomas, and six sarcomas were evaluated for the presence of CMV by polymerase chain reaction (PCR), in situ hybridization, and immunohistochemical methods. None of the brain tumors or normal brain tissue tested demonstrated evidence of CMV pp65 or early nuclear proteins by immunohistochemistry. In addition, no CMV RNA or DNA was detected in these cases by in situ hybridization and PCR. None of the carcinomas or sarcomas evaluated were positive for CMV by immunohistochemistry, in situ hybridization, or PCR. The findings of the present study suggest that CMV is not significantly associated with brain tumors in humans.

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Quantitation of human cytomegalovirus genomes in the brain of AIDS patients

Cited by 25 publications

References 45 publications

Detection of human cytomegalovirus in normal and neoplastic breast epithelium

Detection of human cytomegalovirus in normal and neoplastic breast epithelium

Is Cytomegalovirus Associated With Human Colorectal Tumorigenesis?

Lack of association of cytomegalovirus with human brain tumors

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