Quantitation of human cytomegalovirus DNA in plasma using an affordable in-house qPCR assay

Khansarinejad, Behzad; Soleimanjahi, Hoorieh; Samiee, Siamak Mirab; Hamidieh, Amir Ali; Paryan, Mahdi; Sanahmadi, Yadollah

doi:10.1016/j.jviromet.2012.04.010

Cited by 20 publications

(17 citation statements)

References 25 publications

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“…The IFA assay results suggested that not only RTx recipients, but also healthy volunteers, were infected by HCMV, because sera anti‐HCMV IgG antibody could be found in both recipients and volunteers, which was concordant with previous reports . It was also observed that titers of sera anti‐HCMV IgG antibody in the positive group were significantly higher than that in the negative group, a much smaller amount of sera anti‐HCMV IgM antibody was detected in the positive group, and no sera anti‐HCMV IgM was found in the negative group and healthy volunteers.…”

Section: Discussionsupporting

confidence: 89%

B‐cell‐activating factor code and human cytomegalovirus infection in renal transplant recipients

Dong

et al. 2014

Microbiology and Immunology

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The objective of the present study was to explore the correlation between the BAFF signal and HCMV-TLR activation in RTx recipients complicated by HCMV. Peripheral blood (anticoagulated by EDTA-Na 2 ) and urine of 113 RTx recipients were collected; healthy volunteers were controlled. Urine HCMV-DNA was detected by real-time PCR. Recipients were classified into a positive group (>10,000 copies/mL urine) and a negative group (<10,000 copies/mL urine). ELISA results showed that sBAFF, sera anti-HCMV pp65 immunoglobulin (Ig)G antibody, and total IgG all significantly increased in recipients with positive HCMV-DNA (>10,000 copies/mL urine) (P < 0.05) compared with negative recipients (<10,000 copies/mL urine). In the positive group, HCMV-DNA copies and total IgG positively correlated with sBAFF (r ¼ 0.988 and 0.625, respectively) (P < 0.05). Luminex assay results suggested that the incidence of anti-HLA I and II and MICA antibody obviously increased in positive recipients. The expression level of BAFF and BAFF-R increased in positive recipients. A total of 88 particular genes-involved in TLR signaling pathways, NF-kB signaling pathways, and cytokinecytokine receptor signaling pathways-were detected in real-time PCR chip assay. A total of 46 genes were differentially expressed greater than two-fold, and the expression characteristic of BAFF-R was concordant with FACS results. Our findings are that activation of HCMV would induce or enhance the activation of BAFF code in RTx recipients, which may independently or cooperatively participate in renal allograft injury and decrease the long-term outcome of renal allografts.

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Section: Discussionsupporting

confidence: 89%

B‐cell‐activating factor code and human cytomegalovirus infection in renal transplant recipients

Dong

et al. 2014

Microbiology and Immunology

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“…Species identification relied on MALDI-TOF technique combined with classical biochemical identification methods. are designed based on sequences and assays published elsewhere [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. Table 1 gives a list of LDTs used for detection of pathogens included in the FilmArray ME with underlying references.…”

Section: Ethicsmentioning

confidence: 99%

Implementation of the FilmArray ME panel in laboratory routine using a simple sample selection strategy for diagnosis of meningitis and encephalitis

Pfefferle

Martin

Aepfelbacher

et al. 2020

Preprint

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“…Primary infections are shown by low IgG avidity and seroconversion (Bodeus et al, 1998;Bodeus & Goubau, 1999;Yinon et al, 2010;Revello et al, 2011). Since the late 1990s, quantitation of HCMV DNA load by real-time PCR assays has become the most widely used diagnostic tool (Sia et al, 2000;Atkinson & Emery, 2011;Mengelle et al, 2011;Khansarinejad et al, 2012). Since the late 1990s, quantitation of HCMV DNA load by real-time PCR assays has become the most widely used diagnostic tool (Sia et al, 2000;Atkinson & Emery, 2011;Mengelle et al, 2011;Khansarinejad et al, 2012).…”

Section: Tlrs Contribute To Immune Response Against Hcmvmentioning

confidence: 99%

“…However, diagnosis of these infections by seroconversion determination requires completing screening tests in early pregnancy, which is not practiced routinely. Since the late 1990s, quantitation of HCMV DNA load by real-time PCR assays has become the most widely used diagnostic tool (Sia et al, 2000;Atkinson & Emery, 2011;Mengelle et al, 2011;Khansarinejad et al, 2012). This is a rapid, efficient and sensitive method for determination of HCMV viremia level in newborns and HCMV viral copy number in amniotic fluid and placental cells, as well as urine and saliva samples from pregnant women with active HCMV infections and their newborns within the first week of birth (Paradowska et al, 2006(Paradowska et al, , 2012Ducroux et al, 2008;Kouri et al, 2010).…”

mentioning

confidence: 99%

Alterations inTLRsas new molecular markers of congenital infections withHuman cytomegalovirus?

2013

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Toll-like receptors (TLRs) play a crucial role in non-specific immunity against various infections. The most common intrauterine infection, caused by Human cytomegalovirus (HCMV), results in perinatal morbidity and mortality of primary infected fetuses. The induction of immune response by TLRs was observed in HCMV infections in murine models and cell lines cultured in vitro. Studies reported an immunological response in pregnant women with primary HCMV infection and TLR2 activity in collecting of HCMV particles in placental syncytiotrophoblasts (STs) in vivo and cultured ST, and in stimulation of tumor necrosis factor (TNF)-α expression and damage of villous trophoblast. Expression levels of TLRs are associated with cell type, stage of pregnancy and response to microorganisms. We show the effect of HCMV infection on the development of pregnancy as well as the effect of TLR single-nucleotide polymorphisms on the occurrence and course of infectious diseases, immune response and diseases of pregnancy. We report the impact of TLRs on the function of miRNAs and the altered expression levels of these molecules, as observed in HCMV infections. We suggest that the methylation status of TLR gene promoter regions as epigenetic modifications may be significant in the immune response to HCMV infections. We conclude that it is important to study in detail the molecular mechanisms of TLR function in the immune response to HCMV infections in pregnancy.

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Quantitation of human cytomegalovirus DNA in plasma using an affordable in-house qPCR assay

Cited by 20 publications

References 25 publications

B‐cell‐activating factor code and human cytomegalovirus infection in renal transplant recipients

B‐cell‐activating factor code and human cytomegalovirus infection in renal transplant recipients

Implementation of the FilmArray ME panel in laboratory routine using a simple sample selection strategy for diagnosis of meningitis and encephalitis

Alterations inTLRsas new molecular markers of congenital infections withHuman cytomegalovirus?

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