2012
DOI: 10.1016/j.jviromet.2012.04.010
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Quantitation of human cytomegalovirus DNA in plasma using an affordable in-house qPCR assay

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Cited by 20 publications
(17 citation statements)
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“…The IFA assay results suggested that not only RTx recipients, but also healthy volunteers, were infected by HCMV, because sera anti‐HCMV IgG antibody could be found in both recipients and volunteers, which was concordant with previous reports . It was also observed that titers of sera anti‐HCMV IgG antibody in the positive group were significantly higher than that in the negative group, a much smaller amount of sera anti‐HCMV IgM antibody was detected in the positive group, and no sera anti‐HCMV IgM was found in the negative group and healthy volunteers.…”
Section: Discussionsupporting
confidence: 89%
“…The IFA assay results suggested that not only RTx recipients, but also healthy volunteers, were infected by HCMV, because sera anti‐HCMV IgG antibody could be found in both recipients and volunteers, which was concordant with previous reports . It was also observed that titers of sera anti‐HCMV IgG antibody in the positive group were significantly higher than that in the negative group, a much smaller amount of sera anti‐HCMV IgM antibody was detected in the positive group, and no sera anti‐HCMV IgM was found in the negative group and healthy volunteers.…”
Section: Discussionsupporting
confidence: 89%
“…Species identification relied on MALDI-TOF technique combined with classical biochemical identification methods. are designed based on sequences and assays published elsewhere [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. Table 1 gives a list of LDTs used for detection of pathogens included in the FilmArray ME with underlying references.…”
Section: Ethicsmentioning
confidence: 99%
“…Primary infections are shown by low IgG avidity and seroconversion (Bodeus et al, 1998;Bodeus & Goubau, 1999;Yinon et al, 2010;Revello et al, 2011). Since the late 1990s, quantitation of HCMV DNA load by real-time PCR assays has become the most widely used diagnostic tool (Sia et al, 2000;Atkinson & Emery, 2011;Mengelle et al, 2011;Khansarinejad et al, 2012). Since the late 1990s, quantitation of HCMV DNA load by real-time PCR assays has become the most widely used diagnostic tool (Sia et al, 2000;Atkinson & Emery, 2011;Mengelle et al, 2011;Khansarinejad et al, 2012).…”
Section: Tlrs Contribute To Immune Response Against Hcmvmentioning
confidence: 99%
“…However, diagnosis of these infections by seroconversion determination requires completing screening tests in early pregnancy, which is not practiced routinely. Since the late 1990s, quantitation of HCMV DNA load by real-time PCR assays has become the most widely used diagnostic tool (Sia et al, 2000;Atkinson & Emery, 2011;Mengelle et al, 2011;Khansarinejad et al, 2012). This is a rapid, efficient and sensitive method for determination of HCMV viremia level in newborns and HCMV viral copy number in amniotic fluid and placental cells, as well as urine and saliva samples from pregnant women with active HCMV infections and their newborns within the first week of birth (Paradowska et al, 2006(Paradowska et al, , 2012Ducroux et al, 2008;Kouri et al, 2010).…”
mentioning
confidence: 99%