Quantitation of elastin in human urine and rat pleural mesothelial cell matrix by a sensitive avidin-biotin ELISA for desmosine

Laurent, Philippe; Magne, L; Palmas, J De; Bignon, J.; Jaurand, Marie‐Claude

doi:10.1016/0022-1759(88)90002-6

Cited by 30 publications

(14 citation statements)

References 20 publications

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“…Mesothelial cells also have the capacity to synthesize a variety of ECM macromolecules in vitro . Cultured rat mesothelial cells produce collagen types I, III and IV, elastin, fibronectin and laminin 170–175 . Furthermore, electron microscopy studies reveal that pleural mesothelial cells can organise these components into complex structures that resemble components of the ECM in vivo (thick collagen fibres, the amorphous components of elastic fibres and basement membrane‐like structures), which were restricted to the basal region below the cells in culture 170 .…”

Section: Tissue Repairmentioning

confidence: 99%

“…Cultured rat mesothelial cells produce collagen types I, III and IV, elastin, fibronectin and laminin. [170][171][172][173][174][175] Furthermore, electron microscopy studies reveal that pleural mesothelial cells can organise these components into complex structures that resemble components of the ECM in vivo (thick collagen fibres, the amorphous components of elastic fibres and basement membrane-like structures), which were restricted to the basal region below the cells in culture. 170 Several studies have also demonstrated increased production of these molecules following incubation of cells with various cytokines and growth factors, such as TNF-a, EGF and PDGF.…”

Section: Tissue Repairmentioning

confidence: 99%

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Mesothelial cells: Their structure, function and role in serosal repair

2002

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Mesothelial cells: Their structure, function and role in serosal repair MUTSAERS SE. Respirology 2002; 7: 171-191 The mesothelium is composed of an extensive monolayer of specialized cells (mesothelial cells) that line the body's serous cavities and internal organs. Traditionally, this layer was thought to be a simple tissue with the sole function of providing a slippery, non-adhesive and protective surface to facilitate intracoelomic movement. However, with the gradual accumulation of information about serosal tissues over the years, the mesothelium is now recognized as a dynamic cellular membrane with many important functions. These include transport and movement of fluid and particulate matter across the serosal cavities, leucocyte migration in response to inflammatory mediators, synthesis of pro-inflammatory cytokines, growth factors and extracellular matrix proteins to aid in serosal repair, release of factors to promote both the deposition and clearance of fibrin, and antigen presentation. Furthermore, the secretion of molecules, such as glycosaminoglycans and lubricants, not only protects tissues from abrasion, but also from infection and possibly tumour dissemination. Mesothelium is also unlike other epithelial-like surfaces because healing appears diffusely across the denuded surface, whereas in true epithelia, healing occurs solely at the wound edges as sheets of cells. Although controversial, recent studies have begun to shed light on the mechanisms involved in mesothelial regeneration. In the present review, the current understanding of the structure and function of the mesothelium and the biology of mesothelial cells is discussed, together with recent insights into the mechanisms regulating its repair.

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Section: Tissue Repairmentioning

confidence: 99%

Section: Tissue Repairmentioning

confidence: 99%

Mesothelial cells: Their structure, function and role in serosal repair

2002

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“…Thus, while the normal processes of degradation and regeneration of the elastin network involved in tissue renewal produce a very low urinary excretion of these cross-links, their level was shown to increase significantly in the urine of patients affected by pulmonary diseases [4,[8][9][10]. Given the possibility that desmosines could be used as potential markers of elastin alteration, a variety of immunochemical (radioimmunoassay (RIA) [11][12][13][14]; enzyme-linked immunosorbent assay, ELISA [15][16][17]) and chromatographic (reverse-phase high-performance liquid chromatography (RP-HPLC) [8,[18][19][20]) procedures were developed to detect their amount in the urine. Although of great utility, some limitations have to be dealt with in these approaches.…”

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confidence: 99%

Capillary electrophoresis with laser‐induced fluorescence detection as a novel sensitive approach for the analysis of desmosines in real samples

et al. 2004

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Among various biomarkers believed to behave as descriptors of the disease process in chronic obstructive pulmonary disease (COPD), urinary desmosines are commonly used for monitoring elastin degradation. Given the low concentrations of urinary desmosines, their quantitative determination in this biological matrix often requires preconcentration steps. To minimize both solute losses and effects of sample matrix, and to decrease data variability related to the above-mentioned manipulation processes, we have developed a capillary electrophoresis approach combined with laser-induced fluorescence (CE-LIF) detection system using urine samples not submitted to any pretreatment procedure other than filtering the sample. Urines were hydrolyzed, derivatized with fluorescein isothiocyanate (FITC) and endogenous desmosines were identified by addition of standard analytes and submitting to mass spectrometry (MS) analysis the material collected from micropreparative runs. The assay showed good linearity, reproducibility and precision, allowing to detect amounts of desmosines as low as 10(-8) M (equivalent to 0.1 fmol on column). We conclude that CE-LIF technique is a highly sensitive method for detecting urinary desmosines.

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“…The ELISA method developed by Laurent et al [30] was of particular interest. Based on the competition between solid phase-bound desmosine-protein conjugate and free desmosine for binding to monospecific antidesmosine antiserum, it showed greater sensitivity and specificity than those previously described.…”

Section: Elisamentioning

confidence: 99%

Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

Viglio

Annovazzi

Luisetti

et al. 2007

J of Separation Science

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Desmosines are crosslinking amino acids unique to mature elastin in humans. Owing to this unicity, they have been discussed as potentially attractive indicators of connective tissue disorders whose clinical manifestations are mostly the result of elastin degradation. This review covers advances in immunochemical, chromatographic, and electrophoretic procedures applied in the last 25 years to detect and quantitate these crosslinksin a variety of biological samples. Recent applications of CE with LIF detection (CE-LIF) for investigating the content of desmosines in different fluids will also be discussed.

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Quantitation of elastin in human urine and rat pleural mesothelial cell matrix by a sensitive avidin-biotin ELISA for desmosine

Cited by 30 publications

References 20 publications

Mesothelial cells: Their structure, function and role in serosal repair

Mesothelial cells: Their structure, function and role in serosal repair

Capillary electrophoresis with laser‐induced fluorescence detection as a novel sensitive approach for the analysis of desmosines in real samples

Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

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