Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine

Gonzalez, Diego Daniel; Rimondi, Agustina; Aguirreburualde, María Sol Pérez; Mozgovoj, Marina Valeria; Bellido, Demian; Wigdorovitz, Andrés; Santos, María José Dus

doi:10.1016/j.rvsc.2013.03.016

Cited by 11 publications

(4 citation statements)

References 33 publications

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“…However, transfer of cytokines and leukocytes via colostrum and milk in other animal models was reported previously (55,56). In our studies, we observed that vaccinated sows possess a high level of mainly proinflammatory cytokines in their serum and colostrum/milk in addition to antibodies.…”

Section: Maternal Cytokines and Immunity To Pertussissupporting

confidence: 60%

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets

et al. 2017

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Maternal vaccination represents a potential strategy to protect both the mother and the offspring against life-threatening infections. This protective role has mainly been associated with antibodies, but the role of cell-mediated immunity, in particular passively transferred cytokines, is not well understood. Here, using a pertussis model, we have demonstrated that immunization of pregnant sows with heat-inactivated bacteria leads to induction of a wide range of cytokines (e.g., tumor necrosis factor alpha [TNF-␣], gamma interferon [IFN-␥], interleukin-6 [IL-6], IL-8, and IL-12/IL-23p40) in addition to pertussis-specific antibodies. These cytokines can be detected in the sera and colostrum/milk of vaccinated sows and subsequently were detected at significant levels in the serum and bronchoalveolar lavage fluid of piglets born to vaccinated sows together with pertussis-specific antibodies. In contrast, active vaccination of newborn piglets with heat-inactivated bacteria induced high levels of specific IgG and IgA but no cytokines. Although the levels of antibodies in vaccinated piglets were comparable to those of passively transferred antibodies, no protection against Bordetella pertussis infection was observed. Thus, our results demonstrate that a combination of passively transferred cytokines and antibodies is crucial for disease protection. The presence of passively transferred cytokines/antibodies influences the cytokine secretion ability of splenocytes in the neonate, which provides novel evidence that maternal immunization can influence the newborn's cytokine milieu and may impact immune cell differentiation (e.g., Th1/Th2 phenotype). Therefore, these maternally derived cytokines may play an essential role both as mediators of early defense against infections and possibly as modulators of the immune repertoire of the offspring.

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Section: Maternal Cytokines and Immunity To Pertussissupporting

confidence: 60%

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets

et al. 2017

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“…Milk somatic cells consist of polymorphonuclear neutrophilic leukocytes, macrophages, lymphocytes, and a small amount of mammary epithelial cells (Gonzalez et al, 2013). Under normal physiological conditions, 1 mL of Holstein milk contains from 2 × 10 4 to 2 × 10 5 somatic cells (VanBaale et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Novel extraction method of genomic DNA suitable for long-fragment amplification from small amounts of milk

Liu

Gao

Yang

et al. 2014

Journal of Dairy Science

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Isolation of genomic DNA is a prerequisite for assessment of milk quality. As a source of genomic DNA, milk somatic cells from milking ruminants are practical, animal friendly, and cost-effective sources. Extracting DNA from milk can avoid the stress response caused by blood and tissue sampling of cows. In this study, we optimized a novel DNA extraction method for amplifying long (>1,000 bp) DNA fragments and used it to evaluate the isolation of DNA from small amounts of milk. The techniques used for the separation of milk somatic cell were explored and combined with a sodium dodecyl sulfate (SDS)-phenol method for optimizing DNA extraction from milk. Spectrophotometry was used to determine the concentration and purity of the extracted DNA. Gel electrophoresis and DNA amplification technologies were used for to determine DNA size and quality. The DNA of 112 cows was obtained from milk (samples of 13 ± 1 mL) and the corresponding optical density ratios at 260:280 nm were between 1.65 and 1.75. Concentrations were between 12 and 45 μg/μL and DNA size and quality were acceptable. The specific PCR amplification of 1,019- and 729-bp bovine DNA fragments was successfully carried out. This novel method can be used as a practical, fast, and economical mean for long genomic DNA extraction from a small amount of milk.

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“…Os efeitos das citocinas derivadas do colostro sobre o sistema imune dos recém-nascidos são pouco conhecidos, entretanto, Hagiwara et al (2001) demonstraram que a IL-1β promove ativação de linfócitos e neutrófilos em bezerros. Gonzalez et al (2013) vacinaram vacas com duas vacinas experimentais contendo o rotavírus ou apenas a proteína VP6. Nesta pesquisa, as células mononucleares do colostro foram isoladas e avaliadas quanto à expressão das citocinas IL-4, IL-6, IL-10, IL-12, IFN-γ e IFN-α.…”

Section: Células: Os Componentes Esquecidos Do Colostrounclassified

Colostro bovino: muito além das imunoglobulinas

Gomes¹,

Baccili²,

Martin³

et al. 2017

Rev. Acad. Ciên. Anim.

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O objetivo desta revisão é trazer informações atualizadas sobre o papel dos fatores imunes do colostro, além das imunoglobulinas (Igs), na proteção direta ou indireta dos bezerros. Os recém-nascidos são hipogamaglobulinêmicos e imunossuprimidos, além de possuírem sistema imune adaptativo näive. Desta forma, é consensual que a colostragem é prioridade no sistema de criação de bezerras, entretanto as novas descobertas sobre a atuação dos leucócitos maternos e seus subprodutos (citocinas), amplificando os mecanismos da resposta imunes inata e adaptativa, tem gerado muitas discussões no meio científico internacional. Além disso, as recentes descobertas sobre o microbioma humano têm sugerido que o processo de amamentação é fundamental para a transferência vertical de Lactobacillus e Bifidobacterium, microrganismos que desempenham um importante papel no desenvolvimento da imunidade de mucosas e saúde intestinal. Em geral, estas descobertas possuem aplicabilidade e trazem reflexões sobre o manejo tradicionalmente empregado na criação de bezerras, considerando-se o contato entre a vaca e recém-nascido, colostragem, sistema de aleitamento, higienização e desinfecção ambiental.

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Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine

Cited by 11 publications

References 33 publications

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets

Novel extraction method of genomic DNA suitable for long-fragment amplification from small amounts of milk

Colostro bovino: muito além das imunoglobulinas

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