Quantitation of cytidine‐5′‐monophospho‐<i>N</i>‐acetylneuraminic acid in human leukocytes using LC–MS/MS: method development and validation

Meng, Fan‐hao; Xu, Xin; Zhang, Michael; Shi, Yifan; Gu, Guodong; Liu, Wanjing; Class, Bradley; Ciccone, Carla; Gahl, William A.; Huizing, Marjan; Carrillo-Carrasco, Nuria; Wang, Amy Q.

doi:10.1002/bmc.4735

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…After centrifugation, the blood sample was preserved in a freezer − 80°C for subsequent analysis. The serum Neu5Ac concentration in each patient was detected by liquid chromatography-tandem mass spectrometry, as previously described [19,23].…”

Section: Measurement Of Serum Neu5acmentioning

confidence: 99%

Correlation of serum N-Acetylneuraminic acid with the risk and prognosis of acute coronary syndrome: a prospective cohort study

Shaohuan

Yao

et al. 2020

BMC Cardiovasc Disord

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Background N-acetylneuraminic acid (Neu5Ac) is a functional metabolite involved in coronary artery disease (CAD). We aimed to evaluate the relationship between serum Neu5Ac and the risk and prognosis of acute coronary syndrome (ACS) in a real-world prospective study. Methods Patients with suspected ACS who underwent coronary angiography were included. Serum Neu5Ac was measured at admission. Coronary lesion severity was evaluated by Gensini Score. GRACE risk stratification was performed at admission. Major adverse cardiac events (MACEs) were recorded during follow-up. Results A total of 766 patients, including 537 with unstable angina (UAP), 100 with myocardial infarction (MI), and 129 without CAD were included. The circulating Neu5Ac level was significantly higher in patients with MI (median [1QR]: 297[220, 374] ng/ml) than in those with UAP (227 [114, 312] ng/ml) or without CAD (207 [114, 276] ng/ml; both p < 0.001). Serum level of Neu5Ac was positively correlated with age, hypertension, serum uric acid, creatinine, MB isoform of creatine kinase (CK-MB), and Gensini score (all p < 0.05). Receiver operating characteristic curve analysis showed that a higher serum Neu5Ac was potentially associated with MI and high-risk GRACE stratification in ACS patients. Logistic analysis identified only elevated serum Neu5Ac as an independent predictor of MACEs in these patients (odds ratio [OR]: 1.003, 95% confidence interval [CI]: 1.002–1.005, p < 0.001). Conclusions Serum Neu5Ac is associated with myocardial injury, GRACE risk category, and prognosis in ACS patients.

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Section: Measurement Of Serum Neu5acmentioning

confidence: 99%

Correlation of serum N-Acetylneuraminic acid with the risk and prognosis of acute coronary syndrome: a prospective cohort study

Shaohuan

Yao

et al. 2020

BMC Cardiovasc Disord

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“…Intracellular concentrations of ManNAc in the cerebral organoids at different time points were detected by using a triple-quadrupole liquid-chromatography tandem mass spectrometry system (LCMS-8060NX, Shimadzu, Japan) coupled with an LC-40D XS system, as previously reported ( 59 ). The column was an ACQUITY UPLC BEH Amide column (2.1 mm by 50 mm, 1.7 μm).…”

Section: Methodsmentioning

confidence: 99%

Neurodevelopmental defects in human cortical organoids with N -acetylneuraminic acid synthase mutation

Bu,

Dai,

Zhang

et al. 2023

Sci. Adv.

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Biallelic genetic variants in N -acetylneuraminic acid synthase ( NANS ), a critical enzyme in endogenous sialic acid biosynthesis, are clinically associated with neurodevelopmental disorders. However, the mechanism underlying the neuropathological consequences has remained elusive. Here, we found that NANS mutation resulted in the absence of both sialic acid and protein polysialylation in the cortical organoids and notably reduced the proliferation and expansion of neural progenitors. NANS mutation dysregulated neural migration and differentiation, disturbed synapse formation, and weakened neuronal activity. Single-cell RNA sequencing revealed that NANS loss of function markedly altered transcriptional programs involved in neuronal differentiation and ribosomal biogenesis in various neuronal cell types. Similarly, Nans heterozygous mice exhibited impaired cortical neurogenesis and neurobehavioral deficits. Collectively, our findings reveal a crucial role of NANS-mediated endogenous sialic acid biosynthesis in regulating multiple features of human cortical development, thus linking NANS mutation with its clinically relevant neurodevelopmental disorders.

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“…After centrifugation, the blood samples were stored in a freezer at −80 °C until further analysis. Serum Neu5Ac concentrations in each patient and control subject were identified using liquid chromatography–tandem mass spectrometry, according to previous research [ 22 , 23 ]. We also detected RNF213 p.R4810K variant with the primer designation of RNF213-4810F 5′-GCCCTCCATTTCTAGCACAC-3′ and RNF213-4810R 5′-AGCTGTGGCGAAAGCTTCTA-3′.…”

Section: Methodsmentioning

confidence: 99%

Correlation of Serum N-Acetylneuraminic Acid with the Risk of Moyamoya Disease

Liu

Zeng

et al. 2023

Brain Sciences

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N-acetylneuraminic acid (Neu5Ac) is a functional metabolite and has been demonstrated to be a risk factor for cardiovascular diseases. It is not clear whether Neu5Ac is associated with a higher risk of cerebrovascular disorders, especially moyamoya disease (MMD). We sought to elucidate the association between serum Neu5Ac levels and MMD in a case–control study and to create a clinical risk model. In our study, we included 360 MMD patients and 89 matched healthy controls (HCs). We collected the participants’ clinical characteristics, laboratory results, and serum Neu5Ac levels. Increased level of serum Neu5Ac was observed in the MMD patients (p = 0.001). After adjusting for traditional confounders, the risk of MMD (odds ratio [OR]: 1.395; 95% confidence interval [CI]: 1.141–1.706) increased with each increment in Neu5Ac level (per μmol/L). The area under the curve (AUC) values of the receiver operating characteristic (ROC) curves of the basic model plus Neu5Ac binary outcomes, Neu5Ac quartiles, and continuous Neu5Ac are 0.869, 0.863, and 0.873, respectively. Furthermore, including Neu5Ac in the model offers a substantial improvement in the risk reclassification and discrimination of MMD and its subtypes. A higher level of Neu5Ac was found to be associated with an increased risk of MMD and its clinical subtypes.

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Quantitation of cytidine‐5′‐monophospho‐N‐acetylneuraminic acid in human leukocytes using LC–MS/MS: method development and validation

Cited by 6 publications

References 20 publications

Correlation of serum N-Acetylneuraminic acid with the risk and prognosis of acute coronary syndrome: a prospective cohort study

Correlation of serum N-Acetylneuraminic acid with the risk and prognosis of acute coronary syndrome: a prospective cohort study

Neurodevelopmental defects in human cortical organoids with N -acetylneuraminic acid synthase mutation

Correlation of Serum N-Acetylneuraminic Acid with the Risk of Moyamoya Disease

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