Quantitation of Cu-containing metallothionein by a Cd-saturation method

Klein, Dominik; Bartsch, Rüdiger; Summer, K

doi:10.1016/0003-2697(90)90040-g

Cited by 68 publications

(22 citation statements)

References 16 publications

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“…As the latter transporter is dysfunctional in WD, a large part of the intracellular copper accumulates in the cytosolic MT storage pool (46,47). However, in the Long-Evans Cinnamon (LEC) rat model of WD, it was shown that the cytosol from livers just before hepatitis still contained Cu-unsaturated MTs and thus may still act as antioxidants (47).…”

Section: Discussionmentioning

confidence: 99%

Methanobactin reverses acute liver failure in a rat model of Wilson disease

Lichtmannegger¹,

Leitzinger²,

Wimmer³

et al. 2016

Journal of Clinical Investigation

135

107

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Section: Discussionmentioning

confidence: 99%

Methanobactin reverses acute liver failure in a rat model of Wilson disease

Lichtmannegger¹,

Leitzinger²,

Wimmer³

et al. 2016

Journal of Clinical Investigation

135

107

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“…Homogenates were centrifuged at 100,000 g (60 min, 03C), to obtain a cytosolic fraction. MT determination was carried out by the Cd}Chelex assay and the thiomolybdate assay Klein et al, 1990) which allow the determination of total metal-binding MT, Cu-binding MT, and Cd, Zn-binding MT. For metal determination, a 0.3-ml portion of homogenates was wet-ashed with 1.5 ml of 65% nitric acid.…”

Section: Methodsmentioning

confidence: 99%

Metallothionein as Bioindicator of Freshwater Metal Pollution: European Eel and Brown Trout

Linde

Sanchez-Galan

Vallés-Mota

et al. 2001

Ecotoxicology and Environmental Safety

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“…The rechnique employed was based on the thiomolybdate assay (Klein et al 1990). The protein content in the cytosolic fraction was determined using the Biuret method, with bovine serum albumin as standard.…”

Section: Experimental Protocolmentioning

confidence: 99%

Transfer and accumulation of cadmium, and the level of metallothionein in perfused human placentae

et al. 1991

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The purpose of the present study was to investigate the effect of perfusion with a medium containing 12 or 24 micrograms Cadmium (as CdCl2) per ml on this metal's accumulation, transfer rate and metallothionein (MT) level. The experiments were performed with an isolated lobule of a dually-perfused human term placenta. Placental cell integrity and viability were characterised by their morphology and metabolic function, manifested in the tissue's electron microscopic structure and glucose and oxygen (O2) consumption, respectively. Perfusion with 24 micrograms Cd/ml medium for 5 h resulted in significant elevation in MT. The transfer rate of Cd to the fetal side of the placenta was very slow, and not until 40 min after the addition of Cd into the maternal side was a significant increase in the metal's level observed in the fetal perfusate. Thereafter, the level of the metal increased gradually and reached a steady state about 1 h later, at a level which was less than 1/20th of its concentration in the maternal perfusate. There was a 60-fold increase in Cd level in the cytosolic fraction obtained from the Cd-treated samples. At 12 micrograms Cd/ml no significant changes were noted in morphology, metabolic function and MT content. None of the Cd levels caused a significant change in O2 and glucose consumption, in spite of the fact that with the higher Cd dose the microstructure of the tissue showed some pathological changes. The observed elevation in MT may provide the fetus some protection against the harmful effects of the metal.

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Quantitation of Cu-containing metallothionein by a Cd-saturation method

Cited by 68 publications

References 16 publications

Methanobactin reverses acute liver failure in a rat model of Wilson disease

Methanobactin reverses acute liver failure in a rat model of Wilson disease

Metallothionein as Bioindicator of Freshwater Metal Pollution: European Eel and Brown Trout

Transfer and accumulation of cadmium, and the level of metallothionein in perfused human placentae

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