1990
DOI: 10.1016/0022-1759(90)90358-3
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Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays

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Cited by 45 publications
(26 citation statements)
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“…Fasting glucose 30 and triglyceride 31 levels were determined by routine biochemical methods. Insulin and leptin were determined in serum by commercially available radioimmunoassays (Sorin, Biomedica, and Wak-Chemie, Medical GmbH, respectively) and adipsin by an ELISA according to Oppermann and coworkers, 32 with the following modifications: for coating the plates, monoclonal anti-human factor D IgG (4 g/mL D10/4, Connex GmbH) in 5.5 mmol/L Na 2 CO 3 , 35 mmol/L NaHCO 3 , and 0.01% thimerosal (pH 9.6) was used. Instead of gelatin, 1% BSA was used in the blocking and sample dilution buffers.…”
Section: Anthropometric and Laboratory Measurementsmentioning
confidence: 99%
“…Fasting glucose 30 and triglyceride 31 levels were determined by routine biochemical methods. Insulin and leptin were determined in serum by commercially available radioimmunoassays (Sorin, Biomedica, and Wak-Chemie, Medical GmbH, respectively) and adipsin by an ELISA according to Oppermann and coworkers, 32 with the following modifications: for coating the plates, monoclonal anti-human factor D IgG (4 g/mL D10/4, Connex GmbH) in 5.5 mmol/L Na 2 CO 3 , 35 mmol/L NaHCO 3 , and 0.01% thimerosal (pH 9.6) was used. Instead of gelatin, 1% BSA was used in the blocking and sample dilution buffers.…”
Section: Anthropometric and Laboratory Measurementsmentioning
confidence: 99%
“…Concentrations of immunoglobulin κ light chain were measured by immunoassay (Freelite, The Binding Site, Birmingham, UK). Complement factor D concentrations were measured by ELISA [17]. Dialysate albumin concentrations were measured by enzyme immunoassay (Alpco, Windham, NH, USA).…”
Section: Methodsmentioning
confidence: 99%
“…The normal concentration range of factor Bb is 0.4 to 1.0 g/mL, 31 the mean 0.65 Ϯ 0.23 g/mL, 33 and high levels were found in septic shock (3.7 Ϯ 2.1 g/mL 34 ). Pretreatment levels of factor Bb were within the normal range in 2 amyopathic patients ( Figure 6A-B) and twice as high in one of the myopathic patients studied ( Figure 6C).…”
Section: Attenuation Of Complement Amplification By Lowering the Concmentioning
confidence: 99%