Quantitation of Cell DNA in the Evaluation of Heteroploid Cells as Substrate for the Preparation of Killed Viral Vaccines

Crainic, R; Horodniceanu, F; Barme, M

doi:10.1159/000149200

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…One of the main problems of epidemiological survey of poliomyelitis is to determine the vaccine or nonvaccine origin of poliovirus isolates (29). Several recently published papers have shown that some of the best methods for identification of the genetic origin of poliovirus isolates are oligonucleotide mapping of poliovirus RNA (14,21,23,30) and antigenic characterization by monoclonal antibodies (5,7,(10)(11)(12)24).…”

Section: Discussionmentioning

confidence: 99%

Natural variation of poliovirus neutralization epitopes

Crainic¹,

Couillin²,

Blondel³

et al. 1983

Infect Immun

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Poliovirus-neutralizing monoclonal antibodies were prepared against type 1, type 2, and type 3 wild laboratory (Mahoney, MEF1, and Saukett) and Sabin vaccine strains. Fifty-five poliovirus laboratory strains and field isolates were assayed by neutralization index test with a panel of homotypic monoclonal antibodies. A total of 27 monoclonal antibodies were used. Two categories of neutralization epitopes were found, i.e., cross-reacting (K), which is present on almost all strains of the same serotype, and strain-specific (V, variable), either wild (VW) or Sabin (VS). Several distinct neutralization epitopes were defined for each of the three poliovirus serotypes in almost every category. The study of antigenic variation of the Sabin type 1 vaccine virus during replication in human intestine showed that the VS neutralization epitope may be lost and even replaced by the VW epitopes of the parental Mahoney virus. A late isolate from a vaccine-fed infant recovered the complete neutralization epitope pattern of the Mahoney strain. Upon in vivo virus replication, a different kind of antigenic variation was also detected in which an epitope lost its function in virus neutralization but kept its antigenic conformation unaltered. Neutralization epitope analysis demonstrated that the presence of VS epitopes on a field isolate suggests the Sabin origin of the strain when the isolate displays the same epitope pattern as the original Sabin virus, or confirms it when the VS epitope(s) is mutually exclusive of VW epitopes. The lack of VS epitopes on a field isolate does not rule out its being of Sabin origin.

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