Quantitation and Enzymatic Activity of the Alkaline Protease from <i>Pseudomonas aeruginosa</i> in Culture Supernatants from Clinical Strains

Louis, Dominique; Sorlier, Pierre; Wallach, Jean

doi:10.1515/cclm.1998.050

Cited by 20 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dominique et al [40] isolated alkaline protease from Pseudomonas aeruginosa (EC 3.4.24). The alkaline protease concentration and activity were determined in culture supernatants of pseudomonas from patients with cystic fibrosis.…”

Section: Qualitative and Quantitative Analytical Techniques For Serramentioning

confidence: 99%

Analytical techniques for serratiopeptidase: A review

Gupte

Luthra

2017

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

A review is presented on different analytical techniques used for qualitative and quantitative analysis of serratiopeptidase, a proteolytic enzyme, which has recently gained importance as an anti-inflammatory agent. Efforts have been made to collate all the relevant references to the extent possible. The review discusses the advantages and disadvantages of the cited analytical techniques, which will help to give insights into the methods used for estimation of serratiopeptidase as such, from clinical isolates and from its dosage forms. The review highlights the basic as well as advanced techniques performed for estimating serratiopeptidase. The techniques illustrated here have been demonstrated to be useful for qualitative and quantitative determination of serratiopeptidase and may find application in analyzing other related proteases.

show abstract

Section: Qualitative and Quantitative Analytical Techniques For Serramentioning

confidence: 99%

Analytical techniques for serratiopeptidase: A review

Gupte

Luthra

2017

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

show abstract

“…However, current diagnosis of P. aeruginosa infection largely relies on traditional methods, such as Gram-stain, bacterial culturing, biochemical methods, and immunoassays [ 11 ]. Though those methods are sensitive and reliable, they require a significant amount of time to confirm infection, prolonging the time between patient clinical presentations and antibiotics treatments.…”

Section: Introductionmentioning

confidence: 99%

Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human Serum

Hong

Yancey²,

Battistella

et al. 2015

BioMed Research International

View full text Add to dashboard Cite

Exotoxin A is one of the virulence factors of Pseudomonas aeruginosa, a bacterium that can cause infections resulting in adverse health outcomes and increased burden to health care systems. Current methods of diagnosing P. aeruginosa infections are time consuming and can require significant preparation of patient samples. This study utilized a novel variation of the Systematic Evolution of Ligand by Exponential Enrichment, Decoy-SELEX, to identify an Exotoxin A specific single-stranded DNA (ssDNA) molecular recognition element (MRE). Its emphasis is on increasing stringency in directing binding toward free target of interest and at the same time decreasing binding toward negative targets. A ssDNA MRE with specificity and affinity was identified after fourteen rounds of Decoy-SELEX. Utilizing surface plasmon resonance measurements, the determined equilibrium dissociation constant (K d) of the MRE is between 4.2 µM and 4.5 µM, and is highly selective for Exotoxin A over negative targets. A ssDNA MRE modified sandwich enzyme-linked immunosorbent assay (ELISA) has been developed and achieved sensitive detection of Exotoxin A at nanomolar concentrations in human serum. This study has demonstrated the proof-of-principle of using a ssDNA MRE as a clinical diagnostic tool.

show abstract

“…Two of these, the 50-kDa alkaline protease AprA and the 33-kDa elastase LasB, often are implicated in P. aeruginosa infections. They can be detected under in vitro culture conditions and in vivo at various sites of infection (2)(3)(4)(5). Both AprA and LasB are zinc metalloproteases that require calcium ions for stability (6,7).…”

mentioning

confidence: 99%

The LasB Elastase of Pseudomonas aeruginosa Acts in Concert with Alkaline Protease AprA To Prevent Flagellin-Mediated Immune Recognition

et al. 2016

View full text Add to dashboard Cite

eThe opportunistic pathogen Pseudomonas aeruginosa is capable of establishing severe and persistent infections in various eukaryotic hosts. It encodes a wide array of virulence factors and employs several strategies to evade immune detection. In the present study, we screened the Harvard Medical School transposon mutant library of P. aeruginosa PA14 for bacterial factors that modulate interleukin-8 responses in A549 human airway epithelial cells. We found that in addition to the previously identified alkaline protease AprA, the elastase LasB is capable of degrading exogenous flagellin under calcium-replete conditions and prevents flagellin-mediated immune recognition. Our results indicate that the production of two proteases with anti-flagellin activity provides a failsafe mechanism for P. aeruginosa to ensure the maintenance of protease-dependent immune-modulating functions.

show abstract

Quantitation and Enzymatic Activity of the Alkaline Protease from Pseudomonas aeruginosa in Culture Supernatants from Clinical Strains

Cited by 20 publications

References 31 publications

Analytical techniques for serratiopeptidase: A review

Analytical techniques for serratiopeptidase: A review

Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human Serum

The LasB Elastase of Pseudomonas aeruginosa Acts in Concert with Alkaline Protease AprA To Prevent Flagellin-Mediated Immune Recognition

Contact Info

Product

Resources

About