Abstract:A three-step method for the identification of the main sources of fecal coliforms (FC) in urban waters and for the analysis of remedial actions is proposed. The method is based on (1) The statistical analysis of the relationship between rainfall and FC concentrations in urban rivers; (2) The simulation of hydrology and hydraulics; and (3) Scenario analysis. The proposed method was applied to the Beauport River watershed, in Canada, covering an area of 28.7 km 2 . FC loads and concentrations in the river, durin… Show more
“…This increase in coliforms during the rainy season has been evidenced in several studies, where it is associated with an increase in runoff and wastewater towards the coasts [31]. In addition, this increase in discharges decreases the salinity and increases the temperature of the system, thus favoring bacterial growth [32,33].…”
Coliforms are relatively common in aquatic environments, but their concentrations can be increased by environmental changes and anthropogenic activities, thus impacting fisheries resources. To determine the microbiological quality in the octopus production chain (capture, post-capture, processing and commercialization), total (TC) and fecal (FC) coliforms were quantified in sea water, fresh octopus, fresh water, ice and octopus in two presentations: packed in ice and boiled. Samples came from fishing zones Enmedio, Chopa and La Gallega at the Veracruz Reef System (VRS) during dry, rainy and windy seasons. The coliforms were determined using the most probable number technique (MPN). The most relevant results indicated that octopus packed in ice coming from the commercialization stage had FC levels >540 MPN/100 g, which exceeded the permissible limits (230 MPN/100 g). Therefore, these products present a risk for human consumption. Differences in FC were observed in octopuses between the three fishing zones (H = 8.697; p = 0.0129) and among the three climatic seasons, increasing during the rainy season, highlighting La Gallega with 203.33 ± 63 MPN (H = 7.200; p = 0.0273). The results provide evidence of the environmental and anthropogenic influences on coliform concentrations and the urgent need to implement an efficient cold chain throughout octopus production stages with adequate handling practices to reverse this situation.
“…This increase in coliforms during the rainy season has been evidenced in several studies, where it is associated with an increase in runoff and wastewater towards the coasts [31]. In addition, this increase in discharges decreases the salinity and increases the temperature of the system, thus favoring bacterial growth [32,33].…”
Coliforms are relatively common in aquatic environments, but their concentrations can be increased by environmental changes and anthropogenic activities, thus impacting fisheries resources. To determine the microbiological quality in the octopus production chain (capture, post-capture, processing and commercialization), total (TC) and fecal (FC) coliforms were quantified in sea water, fresh octopus, fresh water, ice and octopus in two presentations: packed in ice and boiled. Samples came from fishing zones Enmedio, Chopa and La Gallega at the Veracruz Reef System (VRS) during dry, rainy and windy seasons. The coliforms were determined using the most probable number technique (MPN). The most relevant results indicated that octopus packed in ice coming from the commercialization stage had FC levels >540 MPN/100 g, which exceeded the permissible limits (230 MPN/100 g). Therefore, these products present a risk for human consumption. Differences in FC were observed in octopuses between the three fishing zones (H = 8.697; p = 0.0129) and among the three climatic seasons, increasing during the rainy season, highlighting La Gallega with 203.33 ± 63 MPN (H = 7.200; p = 0.0273). The results provide evidence of the environmental and anthropogenic influences on coliform concentrations and the urgent need to implement an efficient cold chain throughout octopus production stages with adequate handling practices to reverse this situation.
“…In the present study, we also found mean daily inflow (DSF)—collinear in the statistical models with rain events— was also identified as a significant factor affecting not only E. coli concentrations ( p = 0.005), but also presence ( p = 0.0004) (Supplemental Tables S4 and S5). Thériault and Duchesne (2015) also reported a significant relationship between mean fecal coliform concentrations in the Beauport River Watershed in Quebec City, Canada, and the amount of rainfall observed on the same day and on the day before fecal coliform measurement. In addition, Jones et al (2017) reported that surface water flow and higher daily rainfall were the most important variables for broadly delineating detection of human and animal viruses, as well as F ‐specific coliphage—a fecal indicator for viruses.…”
Section: Resultsmentioning
confidence: 83%
“…The authors observed higher concentrations of FIB in urban runoff when compared with agricultural and mixed land use areas. Additionally, runoff water can overload the capacity of municipal sewage systems, resulting in the discharge of untreated water into nearby receiving rivers (McLellan et al, 2007; Thériault and Duchesne, 2015). Historically a greater issue with combined sewer systems, sanitary sewer systems can be affected by infiltration of rainwater through the subsurface into compromised infrastructure (i.e., cracks, broken pipes, equipment failure, blockages), resulting in sanitary sewer overflows (Arnone and Walling, 2007).…”
Standard methods for the evaluation of recreational water quality rely on generic bacterial indicators such as . However, does not provide enough information to determine fecal source or public health risk. The stsudy objective was to determine factors influencing the presence of and host-specific markers (HSM) from upstream to downstream in Beaver Lake Reservoir (BLR). From February 2014 to September 2015, 420 base flow and rain event samples were collected from seven sites-two sites from streams (White River [WR] and War Eagle Creek) draining into BLR and five sites from within BLR. Each sample was analyzed for and by quantitative polymerase chain reaction for HSM related to human, bovine, and poultry. The data indicate that overall levels of were significantly greater in the WR and significantly lower at the most downstream sampling location in BLR. is more likely present during spring (adjusted odds ratio [aOR] = 1.86), at the WR sampling site (aOR = 3.39), or during a rain event (aOR = 2.73). Moreover, the HSM HumM2 is more likely present (aOR = 1.99) when is present. These same factors were associated with concentrations >126 most probable number 100 mL (aOR = 2.76-12.48), except the poultry marker CL was more likely associated (aOR = 3.81) than HumM2. This study revealed that both seasonal and locational factors are important variables for fecal pollution in BLR. Moreover, these same factors may apply to fecal pollution in manmade reservoirs within similar types of watersheds across the United States, as well as internationally.
“…Microbes in urban stormwater originate from both point and diffuse sources, including those from animal and human faecal pollution [2,5,6]. The urban stormwater runoff can mobilise and transport microbial pathogens from various non-point sources to surface water bodies, such as rivers, lakes and creeks.…”
Microbial pathogens present in stormwater, which originate from human sewage and animal faecal matters, are one of the major impediments in stormwater reuse. The transport of microbes in stormwater is more than just a physical process. The mobility of microbes in stormwater is governed by many factors, such as dissolved organic matter, cations, pH, temperature and water flow. This paper examined the roles of three environmental variables, namely: dissolved organic matter, positive cations and stormwater flow on the transport of two faecal indicator bacteria (FIB), Enterococcus spp. and Escherichia coli. Stormwater runoff samples were collected during twelve wet weather events and one dry weather event from a medium density residential urban catchment in Brisbane. Enterococcus spp. numbers as high as 3 × 104 cfu/100 mL were detected in the stormwater runoff, while Escherichia coli numbers up to 3.6 × 103 cfu/100 mL were observed. The dissolved organic carbon (DOC) in the stormwater samples was in the range of 2.2–5.9 mg/L with an average concentration of 4.5 mg/L in which the hydrophilic carbon constituted the highest mass fraction of 60–80%. The results also showed that the transport of FIB in stormwater was reduced with an increasing concentration of the hydrophilic organic fraction, especially the humic fraction. On the contrary, the concentration of trivalent cations and stormwater flow rate showed a positive correlation with the FIB numbers. These findings indicated the potentiality to make a good use and measurement of simple environmental variables to reflect the degree of microbe transport in stormwater from residential/suburban catchments.
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