Quantifying tensile forces at cell–cell junctions with a DNA-based fluorescent probe

Zhao, Bin; Li, Ningwei; Xie, Tianfa; Bagheri, Yousef; Liang, Chungwen; Keshri, Puspam; Sun, Yubing; Meng, Yuchuan

doi:10.1039/d0sc01455a

Cited by 38 publications

(60 citation statements)

References 61 publications

(80 reference statements)

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“…An alternative mechanoelectric DNA-based probe was developed for potassium detection based on the conformational transition of DNA into a G-quadruplex [132]. Inversely, DNA extension can be programmed to occur above a certain force threshold (<100 pN) and FRET reporter probes have been used to further study mechanotransduction at single cell-ECM and cell-cell adhesion sites [133][134][135][136][137].…”

Section: Nanoscale Mechanical Propertiesmentioning

confidence: 99%

Mechanical Properties of DNA Hydrogels: Towards Highly Programmable Biomaterials

Bush

Veneziano

2021

Applied Sciences

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DNA hydrogels are self-assembled biomaterials that rely on Watson–Crick base pairing to form large-scale programmable three-dimensional networks of nanostructured DNA components. The unique mechanical and biochemical properties of DNA, along with its biocompatibility, make it a suitable material for the assembly of hydrogels with controllable mechanical properties and composition that could be used in several biomedical applications, including the design of novel multifunctional biomaterials. Numerous studies that have recently emerged, demonstrate the assembly of functional DNA hydrogels that are responsive to stimuli such as pH, light, temperature, biomolecules, and programmable strand-displacement reaction cascades. Recent studies have investigated the role of different factors such as linker flexibility, functionality, and chemical crosslinking on the macroscale mechanical properties of DNA hydrogels. In this review, we present the existing data and methods regarding the mechanical design of pure DNA hydrogels and hybrid DNA hydrogels, and their use as hydrogels for cell culture. The aim of this review is to facilitate further study and development of DNA hydrogels towards utilizing their full potential as multifeatured and highly programmable biomaterials with controlled mechanical properties.

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Section: Nanoscale Mechanical Propertiesmentioning

confidence: 99%

Mechanical Properties of DNA Hydrogels: Towards Highly Programmable Biomaterials

Bush

Veneziano

2021

Applied Sciences

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“…We have recently developed a ratiometric DNA probe, termed DNAMeter, to quantify intercellular tensile forces [26] . To validate the performance of FLIM‐MDTP, we wondered how the lifetime measurement‐based quantification results can be compared to those obtained from the ratiometric measurement using DNAMeter.…”

Section: Resultsmentioning

confidence: 99%

“…Our next goal was to apply these dual EC‐FLIM‐MDTPs to study different force ranges of E‐cadherin‐mediated tension. The EC‐FLIM‐MDTP (FAM) or EC‐FLIM‐MDTP (Cy3) used above contains a 25 nt DNA hairpin with 22 % G/C base pairs to detect forces around 4.4 pN [26] . To enable the detection of stronger E‐cadherin tension, we have replaced the EC‐FLIM‐MDTP (Cy3) with a DNA hairpin of the same length but contains 66 % G/C base pairs.…”

Section: Resultsmentioning

confidence: 99%

“…To enable the detection of stronger E‐cadherin tension, we have replaced the EC‐FLIM‐MDTP (Cy3) with a DNA hairpin of the same length but contains 66 % G/C base pairs. A threshold force value of 8.1 pN has been determined for this probe construct [26] . To clarify this difference in the DNA hairpin, we just named these two probes as EC‐22‐FLIM‐MDTP (FAM) and EC‐66‐FLIM‐MDTP (Cy3).…”

Section: Resultsmentioning

confidence: 99%

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Quantitative and Multiplexed Fluorescence Lifetime Imaging of Intercellular Tensile Forces

et al. 2021

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Mechanical interactions between cells have been shown to play critical roles in regulating cell signaling and communications.H owever,t he precise measurement of intercellular forces is still quite challenging,e specially considering the complex environment at cell-cell junctions.Inthis study,we report af luorescence lifetime-based approach to image and quantify intercellular molecular tensions.U sing this method, tensile forces among multiple ligand-receptor pairs can be measured simultaneously.W efirst validated our approach and developed lifetime measurement-based DNAtension probes to image E-cadherin-mediated tension on epithelial cells.T hese probes were then further applied to quantify the correlations between E-cadherin and N-cadherin tensions during an epithelial-mesenchymal transition process.T he modular design of these probes can potentially be used to study the mechanical features of various physiological and pathological processes.

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“…Such devices offer great promise to measure extracellular and intracellular forces noninvasively if they can be targeted to desired locations. 128 …”

Section: Discussionmentioning

confidence: 99%

Quantitative Imaging of Biochemistry in Situ and at the Nanoscale

2020

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Biochemical reactions in eukaryotic cells occur in subcellular, membrane-bound compartments called organelles. Each kind of organelle is characterized by a unique lumenal chemical composition whose stringent regulation is vital to proper organelle function. Disruption of the lumenal ionic content of organelles is inextricably linked to disease. Despite their vital roles in cellular homeostasis, there are large gaps in our knowledge of organellar chemical composition largely from a lack of suitable probes. In this Outlook, we describe how, using organelle-targeted ratiometric probes, one can quantitatively image the lumenal chemical composition and biochemical activity inside organelles. We discuss how excellent fluorescent detection chemistries applied largely to the cytosol may be expanded to study organelles by chemical imaging at subcellular resolution in live cells. DNA-based reporters are a new and versatile platform to enable such approaches because the resultant probes have precise ratiometry and accurate subcellular targeting and are able to map multiple chemicals simultaneously. Quantitatively mapping lumenal ions and biochemical activity can drive the discovery of new biology and biomedical applications.

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Quantifying tensile forces at cell–cell junctions with a DNA-based fluorescent probe

Abstract: Cells are physically contacting with each other. Direct and precise quantification of forces at cell–cell junctions is still challenging. Herein, we have developed a DNA-based ratiometric fluorescent probe, termed DNAMeter,...

Cited by 38 publications

References 61 publications

Mechanical Properties of DNA Hydrogels: Towards Highly Programmable Biomaterials

Mechanical Properties of DNA Hydrogels: Towards Highly Programmable Biomaterials

Quantitative and Multiplexed Fluorescence Lifetime Imaging of Intercellular Tensile Forces

Quantitative Imaging of Biochemistry in Situ and at the Nanoscale

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