Quantifying perivascular sympathetic innervation: Regional differences in male C57BL/6 mice at 3 and 20 months

Long, Jennifer B.; Segal, Steven S.

doi:10.1016/j.jneumeth.2009.07.028

Cited by 23 publications

(32 citation statements)

References 15 publications

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“…TH is the rate-limiting enzyme involved in catecholamines synthesis within the postganglionic nerve termi- nals; therefore, changes in TH have been used as a marker of sympathetic activity and have been extensively used to mark postganglionic sympathetic terminals innervating vessels and tissues [32][33][34] . TH and NE concentration with sympathetic activity in different experimental conditions support the proposition that changes in TH and NE concentration may reflect changes in sympathetic activity [35,36] .…”

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confidence: 99%

Effects of Renal Denervation from the Intima and the Adventitia of Renal Arteries on Renal Sympathetic Nerve Activity in Dogs: A Comparative Study

Bai

Yang²,

Gao³

et al. 2015

Cardiology

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Objectives: This study was designed to observe the efficacy and safety of renal denervation from the inside and outside of renal arteries. Methods: Fourteen beagles were randomly divided into a control group (n = 4) and treatment group (n = 10). One renal artery in every beagle of the treatment group was randomly assigned to an intimal group (10 renal arteries) which underwent percutaneous renal denervation from the inside, and another renal artery was assigned to an adventitial group (10 renal arteries) which underwent renal denervation from the outside by laparotomy. Results: Compared with the intimal group, the renal norepinephrine (NE) concentration in the adventitial group had significantly decreased (p = 0.003) at 3 months postsurgery. Renal artery HE staining showed that the perineurium from the adventitial group appeared thickened. Western blotting showed that renal tissue tyrosine hydroxylase (TH) protein expression in the adventitial group was significantly lower than that in the intimal group (p < 0.01) at 3 months postsurgery. There was a renal artery stenosis and a renal atrophy in the intimal group after 1 month of follow-up. Conclusion: The inhibitory effect on renal sympathetic nerve activity was more effective in the adventitial group than the intimal group, and renal denervation in the former group was safe.

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Section: Color Version Available Onlinementioning

confidence: 99%

Effects of Renal Denervation from the Intima and the Adventitia of Renal Arteries on Renal Sympathetic Nerve Activity in Dogs: A Comparative Study

Bai

Yang²,

Gao³

et al. 2015

Cardiology

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“…In our hands, this anti-TH antibody labels neurons with the expected morphologies and distributions in the medulla and axons with the expected distribution in the spinal cord. This antibody has been used extensively to study catecholaminergic innervation in the brain, spinal cord, and periphery in a variety of species (e.g., Cano et al, 2008;Long and Segal, 2009;Morona and Gonzalez, 2009;Nangle et al, 2009).…”

Section: Antibody Characterizationmentioning

confidence: 99%

Innervation of the rat uterus at estrus: A study in full‐thickness, immunoperoxidase‐stained whole‐mount preparations

Gnanamanickam

Llewellyn‐Smith

2011

J of Comparative Neurology

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The innervation of the nonpregnant rat uterus has been studied in histological sections, which contain only small samples of nerves and are unlikely to afford a complete picture of uterine innervation. Here we used whole-mount preparations of entire full-thickness uterine horns from nonpregnant rats in estrus to visualize autonomic or sensory nerves with peroxidase immunohistochemistry. Immunoreactivity was studied for tyrosine hydroxylase (TH)-labeled sympathetic nerves; vesicular acetylcholine transporter (VAChT), parasympathetic nerves; and substance P (SP) and calcitonin gene-related peptide (CGRP), sensory nerves. Neuropeptide Y (NPY) and nitric oxide synthase (NOS) identified more than one of these functionally distinct nerve types. Axons of all neurochemical classes entered the uterus at the mesometrium and innervated the uterine smooth muscle. The linea uteri, a dense band of longitudinal muscle opposite the mesometrium, contained more TH-, NPY-, CGRP-, and VAChT-immunoreactive axons than the remaining smooth muscle. Axons immunoreactive for NPY, SP, NOS, and VAChT formed a plexus near the circular muscle-endometrium interface. Rare TH- and NPY-immunoreactive axons and occasional CGRP-immunoreactive axons occurred close to uterine glands. Blood vessels had dense perivascular plexuses of TH- and NPY-containing axons and less dense NOS-, SP-, CGRP-, and VAChT-positive plexuses. The circular muscle plexus and glands were absent opposite the mesometrium. Uterine arterioles formed an interconnected network throughout the uterus. This article provides the first comprehensive description of the autonomic and sensory innervation of the nonpregnant rat uterus and will be a foundation for future studies on changes in uterine innervation caused by normal physiological or pathophysiological challenges.

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“…muscle sympathetic nerve activity; blood pressure; large artery vasoconstriction SYMPATHETIC VASOCONSTRICTION is most effective in altering blood flow and blood pressure (BP) by influencing small arteries and arterioles (16, 18). However, conduit arteries also exhibit sympathetic perivascular nerve innervations (11,15,28,33), express ␣-adrenergic receptors (11, 29, 33), and significantly vasoconstrict in response to ␣-adrenergic agonists (3, 40). Indeed, the majority of evidence (2,4,6,23,25,26) suggests that large reflex-mediated increases in muscle sympathetic nerve activity (MSNA) decrease conduit artery diameter, although a few reports indicate only minimal changes (6, 30).…”

mentioning

confidence: 99%

“…However, conduit arteries also exhibit sympathetic perivascular nerve innervations (11,15,28,33), express ␣-adrenergic receptors (11,29,33), and significantly vasoconstrict in response to ␣-adrenergic agonists (3,40). Indeed, the majority of evidence (2,4,6,23,25,26) suggests that large reflex-mediated increases in muscle sympathetic nerve activity (MSNA) decrease conduit artery diameter, although a few reports indicate only minimal changes (6,30).…”

mentioning

confidence: 99%

Influence of spontaneously occurring bursts of muscle sympathetic nerve activity on conduit artery diameter

Fairfax

Padilla

Vianna

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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Large increases in muscle sympathetic nerve activity (MSNA) can decrease the diameter of a conduit artery even in the presence of elevated blood pressure, suggesting that MSNA acts to regulate conduit artery tone. Whether this influence can be extrapolated to spontaneously occurring MSNA bursts has not been examined. Therefore, we tested the hypothesis that MSNA bursts decrease conduit artery diameter on a beat-by-beat basis during rest. Conduit artery responses were assessed in the brachial (BA), common femoral (CFA) and popliteal (PA) arteries to account for regional differences in vascular function. In 20 young men, MSNA, mean arterial pressure (MAP), conduit artery diameter, and shear rate (SR) were continuously measured during 20-min periods of supine rest. Spike-triggered averaging was used to characterize beat-by-beat changes in each variable for 15 cardiac cycles following all MSNA bursts, and a peak response was calculated. Diameter increased to a similar peak among the BA (+0.14 ± 0.02%), CFA (+0.17 ± 0.03%), and PA (+0.18 ± 0.03%) following MSNA bursts (all P < 0.05 vs. control). The diameter rise was positively associated with an increase in MAP in relation to increasing amplitude and consecutive numbers of MSNA bursts (P < 0.05). Such relationships were similar between arteries. SR changes following MSNA bursts were heterogeneous between arteries and did not appear to systematically alter diameter responses. Thus, in contrast to our hypothesis, spontaneously occurring MSNA bursts do not directly influence conduit arteries with local vasoconstriction or changes in shear, but rather induce a systemic pressor response that appears to passively increase conduit artery diameter.

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Quantifying perivascular sympathetic innervation: Regional differences in male C57BL/6 mice at 3 and 20 months

Cited by 23 publications

References 15 publications

Effects of Renal Denervation from the Intima and the Adventitia of Renal Arteries on Renal Sympathetic Nerve Activity in Dogs: A Comparative Study

Effects of Renal Denervation from the Intima and the Adventitia of Renal Arteries on Renal Sympathetic Nerve Activity in Dogs: A Comparative Study

Innervation of the rat uterus at estrus: A study in full‐thickness, immunoperoxidase‐stained whole‐mount preparations

Influence of spontaneously occurring bursts of muscle sympathetic nerve activity on conduit artery diameter

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