Quantifying Nanoparticle–Cell Interactions

Hondow, Nicole; Brown, Andy; Summers, Huw D.; Brown, M. Rowan; Rees, Paul; Holton, Mark D.; Brydson, Rik

doi:10.1017/s143192761400823x

Cited by 3 publications

(4 citation statements)

References 5 publications

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“…The most common techniques employed for this purpose are confocal fluorescence microscopy, TEM and SEM. In particular, TEM allows to reconstruct 3D images and determine the NPs cellular localization by sequential ultrathin specimen sections [ 120 ]. Field emission (FE) SEM can also be used to distinguish between intracellular and externally bound NPs with a resolution of 1 nm [ 121 ], by using different accelerator voltages [ 122 ].…”

Section: Methods For Qualitative and Quantitative Analysis Of Cellmentioning

confidence: 99%

Intracellular Transport of Silver and Gold Nanoparticles and Biological Responses: An Update

Panzarini

Mariano

Carata

et al. 2018

IJMS

101

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Medicine, food, and cosmetics represent the new promising applications for silver (Ag) and gold (Au) nanoparticles (NPs). AgNPs are most commonly used in food and cosmetics; conversely, the main applications of gold NPs (AuNPs) are in the medical field. Thus, in view of the risk of accidentally or non-intended uptake of NPs deriving from the use of cosmetics, drugs, and food, the study of NPs–cell interactions represents a key question that puzzles researchers in both the nanomedicine and nanotoxicology fields. The response of cells starts when the NPs bind to the cell surface or when they are internalized. The amount and modality of their uptake depend on many and diverse parameters, such as NPs and cell types. Here, we discuss the state of the art of the knowledge and the uncertainties regarding the biological consequences of AgNPs and AuNPs, focusing on NPs cell uptake, location, and translocation. Finally, a section will be dedicated to the most currently available methods for qualitative and quantitative analysis of intracellular transport of metal NPs.

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Section: Methods For Qualitative and Quantitative Analysis Of Cellmentioning

confidence: 99%

Intracellular Transport of Silver and Gold Nanoparticles and Biological Responses: An Update

Panzarini

Mariano

Carata

et al. 2018

IJMS

101

View full text Add to dashboard Cite

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“…Visualization of intracellular quantum dots in human U‐2 OS osteosarcoma cell line. (Reprinted with permission from Refs and . Copyright 2013 American Chemical Society; 2014 Microscopy Society of America)…”

Section: Methods For Qualitative and Quantitative Analysis Of Cell‐asmentioning

confidence: 99%

“…The most common techniques employed for identifying intracellular NPs are confocal fluorescence microscopy and TEM. As discussed above, TEM with sequential preparation of ultrathin slices of a specimen can even be used to reconstruct 3D images and subsequently determine the cellular localization of NPs . In addition to TEM techniques, field emission (FE) SEM has been used to distinguish between intracellular and externally bound NPs.…”

Section: Methods To Distinguish Between Cell Surface Bound and Internmentioning

confidence: 99%

“…As discussed above, TEM with sequential preparation of ultrathin slices of a specimen can even be used to reconstruct 3D images and subsequently determine the cellular localization of NPs. 53,54 In addition to TEM techniques, field emission (FE) SEM has been used to distinguish between intracellular and externally bound NPs. This method has an advantage of reducing the sample preparation steps and thus, preparation time while allowing resolution up to 1 nm.…”

Section: Microscopy Methodsmentioning

confidence: 99%

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Methodologies and approaches for the analysis of cell–nanoparticle interactions

Ivask

Mitchell

Malysheva

et al. 2017

WIREs Nanomed Nanobiotechnol

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How to study nanoparticle-cell interactions is the key question that puzzles researchers in the fields of nanomedicine as well as in nanotoxicology. In nanotoxicology, the amount of nanoparticles internalized by the cells or bound to the external surfaces of cells determines the toxic profile of those particles. In medical applications, cellular uptake and binding of medically effective nanoparticles decides their efficacy. Despite the importance of understanding the extent and mode of nanoparticle-cell interactions, these processes are underinvestigated, mainly due to the lack of suitable user-friendly methodologies. Here we discuss the advantages and limitations of currently available (and most advanced) microscopic, spectroscopic, and other bioanalytical methods that could be used to assess cell-nanoparticle interactions either qualitatively or quantitatively. Special emphasis is given to the methods that enable analysis and identification of nanoparticles at single-cell level, and allow intracellular localization and speciation analysis of nanoparticles. This article is categorized under: Nanotechnology Approaches to Biology > Cells at the Nanoscale Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials.

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