Quantifying growth mechanics of living, growing plant cells in situ using microrobotics

Felekis, Dimitrios; Muntwyler, Simon; Vogler, Hannes; Beyeler, Felix; Grossniklaus, Ueli; Nelson, Bradley J.

doi:10.1049/mnl.2011.0024

Cited by 40 publications

(29 citation statements)

References 29 publications

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“…The CFM apparatus is composed primarily of commercially available components: a single-axis capacitive force sensor (Sun and Nelson, 2007) mounted on a three-axis microrobotics actuator (Felekis et al, 2011). The setup is fixed on top of a standard inverted light microscope and isolated from external sources of vibration (Supplemental Fig.…”

Section: Design and Operation Of The Cfm Devicementioning

confidence: 99%

“…S1). Software developed in LabView is used to control the experimental procedure and acquire data (Felekis et al, 2011). A dataanalysis software was developed that automatically extracts the indentation point and apparent stiffness values from the data.…”

Section: Design and Operation Of The Cfm Devicementioning

confidence: 99%

“…Both components are connected to a personal computer via USB and integrated with a custom application implemented in LabView (National Instruments), which controls the positioning system based on a force feedback. The software user interface allows for controlling experiment parameters, such as contact force and maximal force (at deepest indentation), positioning speed, step size for raster scans, and number of loading cycles (Felekis et al, 2011).…”

Section: Cfm Setup Descriptionmentioning

confidence: 99%

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Cellular Force Microscopy for in Vivo Measurements of Plant Tissue Mechanics

et al. 2012

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Although growth and morphogenesis are controlled by genetics, physical shape change in plant tissue results from a balance between cell wall loosening and intracellular pressure. Despite recent work demonstrating a role for mechanical signals in morphogenesis, precise measurement of mechanical properties at the individual cell level remains a technical challenge. To address this challenge, we have developed cellular force microscopy (CFM), which combines the versatility of classical microindentation techniques with the high automation and resolution approaching that of atomic force microscopy. CFM's large range of forces provides the possibility to map the apparent stiffness of both plasmolyzed and turgid tissue as well as to perform micropuncture of cells using very high stresses. CFM experiments reveal that, within a tissue, local stiffness measurements can vary with the level of turgor pressure in an unexpected way. Altogether, our results highlight the importance of detailed physically based simulations for the interpretation of microindentation results. CFM's ability to be used both to assess and manipulate tissue mechanics makes it a method of choice to unravel the feedbacks between mechanics, genetics, and morphogenesis.

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Section: Design and Operation Of The Cfm Devicementioning

confidence: 99%

Section: Design and Operation Of The Cfm Devicementioning

confidence: 99%

Section: Cfm Setup Descriptionmentioning

confidence: 99%

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Cellular Force Microscopy for in Vivo Measurements of Plant Tissue Mechanics

et al. 2012

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“…We used the cellular 342 force microscope (CFM) (Felekis et al, 2011;Vogler et al, 2013) in conjunction with discern direct from secondary effects of the lrx mutations.…”

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confidence: 99%

LRX Proteins Play a Crucial Role in Pollen Grain and Pollen Tube Cell Wall Development

et al. 2017

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“…Microindentation probes provides more flexibility in the scanning range and are capable of applying larger forces, but have not previously used in an automated fashion. Microrobotic manipulation combined with MEMS force sensors has been recently introduced as a promising alternative for mechanical characterization at the cellular level [9]- [11]. Due to the symmetric design of this sensor with its four flexures, parallel motion of the movable body during deflection is possible, making this design superior to most cantilever-type sensors.…”

Section: Introductionmentioning

confidence: 99%

High-throughput analysis of the morphology and mechanics of tip growing cells using a microrobotic platform

Felekis

Vogler

Mecja

et al. 2014

2014 IEEE/RSJ International Conference on Intelligent Robots and Systems

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We present a microrobotic platform that combines MEMS-based capacitive force sensing technology, a dual-stage positioning system and a real-time control and acquisition architecture with computer vision automation to manipulate and mechanically characterize growing plant cells. The topography accuracy of the system, using a silicon wafer sample is measured to be 28 nm (1σ, 200Hz). With an SI-traceable stiffness reference we estimate the accuracy of the RT-CFM to be 3.49%. The target locations are selected from an interactive image of the workspace, and the sensing tip is positioned at each location using visual servoing techniques. Topography and stiffness maps were successfully obtained on growing pollen tubes. With the proposed system, cells can be mechanically stimulated at high speeds and with high precision while the intracellular components are visualized using confocal imaging. The system offers a versatile solution for dexterous and highthroughput characterization of biological specimen.

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Quantifying growth mechanics of living, growing plant cells in situ using microrobotics

Cited by 40 publications

References 29 publications

Cellular Force Microscopy for in Vivo Measurements of Plant Tissue Mechanics

Cellular Force Microscopy for in Vivo Measurements of Plant Tissue Mechanics

LRX Proteins Play a Crucial Role in Pollen Grain and Pollen Tube Cell Wall Development

High-throughput analysis of the morphology and mechanics of tip growing cells using a microrobotic platform

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