Quantifying Demyelination in NK venom treated nerve using its electric circuit model

Abstract

Reduction of myelin in peripheral nerve causes critical demyelinating diseases such as chronic inflammatory demyelinating polyneuropathy, Guillain-Barre syndrome, etc. Clinical monitoring of these diseases requires rapid and non-invasive quantification of demyelination. Here we have developed formulation of nerve conduction velocity (NCV) in terms of demyelination considering electric circuit model of a nerve having bundle of axons for its quantification from NCV measurements. This approach has been validated and demonstrated with toad nerve model treated with crude Naja kaouthia (NK) venom and also shows the effect of Phospholipase A2 and three finger neurotoxin from NK-venom on peripheral nerve. This opens future scope for non-invasive clinical measurement of demyelination.

Conductive Hydrogel for a Photothermal-Responsive Stretchable Artificial Nerve and Coalescing with a Damaged Peripheral Nerve

“…The isolated sciatic nerve and sural muscle of the hoptoad were chosen to verify whether the CPH had a good conductivity and could be used as an implantable nerve substitute ex vivo . For the study of nerve signal conduction, the sciatic nerve isolated from the hoptoad was a classic model in vitro . − For the hoptoad’s sciatic nerve, when the stimulating voltage is beyond the threshold of the resting potential, a contraction of gastrocnemius would be induced by the action potential. The scheme of the experimental setup is shown in Figure A.…”