Quantifying continuous-flow dielectrophoretic trapping of cells and micro-particles on micro-electrode array

Rozitsky, Lichen; Fine, Amir; Dado, Dekel; Nussbaum-Ben-Shaul, Shahar; Levenberg, Shulamit; Yossifon, Gilad

doi:10.1007/s10544-013-9773-9

Cited by 21 publications

(28 citation statements)

References 24 publications

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“…The automated system was tested by trapping and measuring samples of E. coli 5K at a concentration of 2·10 7 cells/ml. Concentration and real-time detection of the trapped bacteria inside the micro-fluidic chip was proven, working with a high flow rate injection of 10µL/min, using inter-digitated electrodes and any type of buffer conductivity [31,32]. Bacteria buffer media conductivity, and its variability, was demonstrated to be a challenging issue to monitoring by means of IA.…”

Section: Discussionmentioning

confidence: 99%

“…Our work presents a totally custom equipment for quick and easy way to concentrate bacteria with DEP technique at relatively high flow rates [31,32], while monitoring its concentration by means of IA technique in a real-time scenario. The presented equipment addresses the issues associated with the combination of these techniques, as well as present a simpler biosensor and custom electronics instrumentation for a future integration of the whole system on a Lab-on-aChip (LoC) device.…”

Section: Introductionmentioning

confidence: 99%

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Combined dielectrophoretic and impedance system for on‐chip controlled bacteria concentration: Application to Escherichia coli

et al. 2015

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Abbreviations: Impedance analysis (IA), escherichia coli (E. coli), digital lock-in (DLIA), analog-to-digital converter (ADC), finite element method (FEM). 2 AbstractThe present paper reports a bacteria autonomous controlled concentrator prototype with a userfriendly interface for bench-top applications. It is based on a micro-fluidic lab-on-a-chip and its associated custom instrumentation, which consists in a dielectrophoretic actuator, to preconcentrate the sample, and an impedance analyser, to measure concentrated bacteria levels.The system is composed by a single micro-fluidic chamber with interdigitated electrodes and custom electronics instrumentation. The prototype is supported by a real-time platform connected to a remote computer which automatically controls the system and displays impedance data which is used to monitor bacteria accumulation status on-chip. The system automates the whole concentrating operation. Performance has been studied for controlled volumes of Escherichia Coli (E. coli) samples injected into the micro-fluidic chip at constant flow rate of 10 µl/min. A media conductivity correcting protocol has been developed, as so as the preliminary results shown the distortion of impedance analyser measurement produced by bacterial media conductivity variations through time. With the correcting protocol, the measured impedance values were related to the quantity of bacteria concentrated with a correlation of 0.988 and a coefficient of variation of 3.1%. Feasibility of E. coli on-chip automated concentration, using the miniaturized system, has been demonstrated and impedance monitoring protocol adjusted and optimized, to handle electrical properties changes of the bacteria media over time.3

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combined dielectrophoretic and impedance system for on‐chip controlled bacteria concentration: Application to Escherichia coli

et al. 2015

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“…However, these devices have the limitation of generating DEP forces near the bottom of the LoC devices limiting their capabilities to trap the overall sample of interest at high flows [19][20]. To solve this issue, works based on using conductive columns to enhance the trapping capabilities have been reported [21][22][23]. However, these techniques imply rather complex chip fabrication and replication techniques.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic concentrator enhancement based on dielectric poles for continuously flowing samples

et al. 2015

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We describe a novel continuous-flow cell concentrator microdevice based on dielectrophoresis, and its associated custom-made control unit. The performances of a classical interdigitated metal electrode-based dielectrophoresis microfluidic device and this enhanced version, that includes insulator-based pole structures, were compared using the same setup. Escherichia coli samples were concentrated at several continuous flows and the device's trapping efficiencies were evaluated by exhaustive cell counts. Our results show that pole structures enhance the retention up to 12.6%, obtaining significant differences for flow rates up to 20 μL/min, when compared to an equivalent classical interdigitated electrodes setup. In addition, we performed a subsequent proteomic analysis to evaluate the viability of the biological samples after the long exposure to the actuating electrical field. No Escherichia coli protein alteration in any of the two systems was observed.

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“…cells shift from attraction (positive DEP) to repulsion (negative DEP), can be used as a sensitive discriminator between different cell types or cell conditions and can be utilized for cell separation and characterization. We have recently employed this method for characterization of cells under continuous flow conditions (Rozitsky et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic characterization of cells in a stationary nanoliter droplet array with generated chemical gradients

Ben-Arye

Park

Shemesh

et al. 2015

Biomed Microdevices

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A novel design of reusable microfluidic platform that generates a stationary nanoliter droplet array (SNDA) for cell incubation and analysis, equipped with a complementary array of individually addressable electrodes for each microwell is studied. Various solute concentration gradients were generated between the wells where dielectrophoresis (DEP) was used to characterize the effect of the gradients on the cell's response. The feasibility of generating concentration gradients and observation of DEP responses was demonstrated using a gradient of salts in combination with microparticles and viable cells. L1210 Lymphoma cells were used as the model cells in these experiments. Lymphoma cells' cross-over frequency (COF) decreased with increasing stress conditions. Specifically, a linear decrease in the cell COF was measured as a function of solution tonicity and blebbistatin dose. Lymphoma cells were incubated under a gradient of the chemotherapeutic agent doxorubicin (DOX), which led to saturation in the cell-COF response at 30 nM DOX, demonstrating the potential of the platform in screening of label-free drugs.

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Quantifying continuous-flow dielectrophoretic trapping of cells and micro-particles on micro-electrode array

Cited by 21 publications

References 24 publications

Combined dielectrophoretic and impedance system for on‐chip controlled bacteria concentration: Application to Escherichia coli

Combined dielectrophoretic and impedance system for on‐chip controlled bacteria concentration: Application to Escherichia coli

Dielectrophoretic concentrator enhancement based on dielectric poles for continuously flowing samples

Dielectrophoretic characterization of cells in a stationary nanoliter droplet array with generated chemical gradients

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