Quantifying cell scattering: The blob algorithm revisited

Kort, Eric J.; Jones, Ashley; Daumbach, Michael; Hudson, Eric A.; Buckner, Bree; Resau, James H.

doi:10.1002/cyto.a.10010

Cited by 6 publications

(7 citation statements)

References 23 publications

(16 reference statements)

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“…Small staining artifacts (mainly resulting from nonstaining endothelial nuclei) were removed by applying a two-pixel opening function. Vessels within the binary mask were identified using connected component analysis (22). Several variables for each connected component within the binary mask were then calculated:…”

Section: Methodsmentioning

confidence: 99%

Preparing the “Soil”: The Primary Tumor Induces Vasculature Reorganization in the Sentinel Lymph Node before the Arrival of Metastatic Cancer Cells

et al. 2006

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Sentinel lymph node (SLN) metastasis is the first step in the spreading of cancer in many malignancies. Tumor-reactive lymphadenopathy in SLNs has been observed for decades, but alterations of the lymphatic channels and vasculature in these nodes before the arrival of metastatic tumor cells remain unexplored. Using animal models, we show here that, before the establishment of metastasis in the SLN, there are reorganizations of the lymphatic channels and the vasculature. The node becomes a functional blood vessel-enriched and lymph vessel/sinus-enriched organ before metastasis. The enlargement of the lymph sinuses is correlated with the primary tumor weight. The newly emerged functional blood vessels develop from high endothelial venules (HEV), in which the proliferation rate of the endothelial cells is also significantly increased. Similar alterations of the HEVs are also characterized in the axillary lymph nodes from human breast cancer patients without the evidence of metastasis. These findings support the hypothesis that modification of the microenvironment for a secondary tumor (i.e., vasculature reorganization in the SLN) can be initiated by a primary tumor before and independent of the physical presence of metastatic cancer cells. (Cancer Res 2006; 66(21): 10365-76)

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Section: Methodsmentioning

confidence: 99%

Preparing the “Soil”: The Primary Tumor Induces Vasculature Reorganization in the Sentinel Lymph Node before the Arrival of Metastatic Cancer Cells

et al. 2006

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“…1A, b). Quantitative analysis of the scattering produced by the addition of plasminogen was performed using a modified Blob algorithm [16] based on numerical quantification of cell clusters (see Materials and methods). The method identifies non-scattered cells as single clusters, whereas scattered cells present as numerous clusters.…”

Section: Resultsmentioning

confidence: 99%

“…Images were downsampled 50% and then automatically thresholded as described elsewhere [16]. Since DIC imaging emphasizes edges, the resulting binary mask required processing to fully capture the cellular regions.…”

Section: Quantification Of Cell Scatteringmentioning

confidence: 99%

Requirement of the enzymatic and signaling activities of plasmin for phorbol-ester-induced scattering of colon cancer cells

Dı́az

Hurtado

Kort

et al. 2006

Experimental Cell Research

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“…Only few attempts have been made to quantify objective measures for cell scattering. Kort et al [24] suggested a simple image-processing application that detects and counts clusters of cells and single cells based on fluorescent marking. Although proving high correlation with manual counting, they do not show a quantitative measure to describe cell scattering.…”

Section: Introductionmentioning

confidence: 99%

Cell Motility Dynamics: A Novel Segmentation Algorithm to Quantify Multi-Cellular Bright Field Microscopy Images

et al. 2011

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Confocal microscopy analysis of fluorescence and morphology is becoming the standard tool in cell biology and molecular imaging. Accurate quantification algorithms are required to enhance the understanding of different biological phenomena. We present a novel approach based on image-segmentation of multi-cellular regions in bright field images demonstrating enhanced quantitative analyses and better understanding of cell motility. We present MultiCellSeg, a segmentation algorithm to separate between multi-cellular and background regions for bright field images, which is based on classification of local patches within an image: a cascade of Support Vector Machines (SVMs) is applied using basic image features. Post processing includes additional classification and graph-cut segmentation to reclassify erroneous regions and refine the segmentation. This approach leads to a parameter-free and robust algorithm. Comparison to an alternative algorithm on wound healing assay images demonstrates its superiority. The proposed approach was used to evaluate common cell migration models such as wound healing and scatter assay. It was applied to quantify the acceleration effect of Hepatocyte growth factor/scatter factor (HGF/SF) on healing rate in a time lapse confocal microscopy wound healing assay and demonstrated that the healing rate is linear in both treated and untreated cells, and that HGF/SF accelerates the healing rate by approximately two-fold. A novel fully automated, accurate, zero-parameters method to classify and score scatter-assay images was developed and demonstrated that multi-cellular texture is an excellent descriptor to measure HGF/SF-induced cell scattering. We show that exploitation of textural information from differential interference contrast (DIC) images on the multi-cellular level can prove beneficial for the analyses of wound healing and scatter assays. The proposed approach is generic and can be used alone or alongside traditional fluorescence single-cell processing to perform objective, accurate quantitative analyses for various biological applications.

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Quantifying cell scattering: The blob algorithm revisited

Cited by 6 publications

References 23 publications

Preparing the “Soil”: The Primary Tumor Induces Vasculature Reorganization in the Sentinel Lymph Node before the Arrival of Metastatic Cancer Cells

Preparing the “Soil”: The Primary Tumor Induces Vasculature Reorganization in the Sentinel Lymph Node before the Arrival of Metastatic Cancer Cells

Requirement of the enzymatic and signaling activities of plasmin for phorbol-ester-induced scattering of colon cancer cells

Cell Motility Dynamics: A Novel Segmentation Algorithm to Quantify Multi-Cellular Bright Field Microscopy Images

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