Quantifying and Adjusting Plasmon‐Driven Nano‐Localized Temperature Field around Gold Nanorods for Nucleic Acids Amplification

You, Minli; Jia, Pengpeng; He, Xiaocong; Wang, Zheyu; Feng, Shangsheng; Ren, Yulin; Li, Zedong; Cao, Lei; Gao, Bin; Chen, Yao; Singamaneni, Srikanth; Xu, Feng

doi:10.1002/smtd.202001254

Cited by 14 publications

(15 citation statements)

References 43 publications

(62 reference statements)

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“…The optical heating of plasmonic NPs to release surface-bound DNA molecules is one of the more studied thermoplasmonic applications. This area of research, in which one strand of DNA is typically covalently bound to the NP surface via the Au–S bond, has been pursued for over a decade now, with work from multiple labs demonstrating DNA release for varying NP shapes, NP concentrations, laser systems, and laser intensities. − DNA has also been used to report on the temperature around gold nanostructures. − For example, You et al used Au–S bond cleavage to back-calculate the surface temperature of gold nanorods under continuous wave laser irradiation . Two general mechanisms have been established for DNA release: (1) thermal melting of double-stranded (ds) DNA with single-stranded (ss) DNA release and (2) Au–S bond cleavage with dsDNA release. ,,,, Focusing on short laser pulse excitation, many of the reports indicate a system-dependent laser pulse energy fluence threshold that promotes Au–S bond cleavage over thermal melting, regardless of the laser pulse width used. ,, This indicates that the DNA release mechanism can be tailored by working at the proper excitation energies.…”

Section: Introductionmentioning

confidence: 99%

“…33−35 For example, You et al used Au−S bond cleavage to backcalculate the surface temperature of gold nanorods under continuous wave laser irradiation. 36 Two general mechanisms have been established for DNA release: (1) thermal melting of double-stranded (ds) DNA with single-stranded (ss) DNA release and (2) Au−S bond cleavage with dsDNA release. 22,24,29,32,37 Focusing on short laser pulse excitation, many of the reports indicate a system-dependent laser pulse energy fluence threshold that promotes Au−S bond cleavage over thermal melting, regardless of the laser pulse width used.…”

Section: ■ Introductionmentioning

confidence: 99%

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Mechanistic Understanding of DNA Denaturation in Nanoscale Thermal Gradients Created by Femtosecond Excitation of Gold Nanoparticles

Hastman

Chaturvedi

et al. 2022

ACS Appl. Mater. Interfaces

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There is significant interest in developing photothermal systems that can precisely control the structure and function of biomolecules through local temperature modulation. One specific application is the denaturation of double-stranded (ds) DNA through femtosecond (fs) laser pulse optical heating of gold nanoparticles (AuNPs); however, the mechanism of DNA melting in these systems is not fully understood. Here, we utilize 55 nm AuNPs with surface-tethered dsDNA, which are locally heated using fs laser pulses to induce DNA melting. By varying the dsDNA distance from the AuNP surface and the laser pulse energy fluence, this system is used to study how the nanosecond duration temperature increase and the steep temperature gradient around the AuNP affect dsDNA dehybridization. Through modifying the distance between the dsDNA and AuNP surface by 3.8 nm in total and the pulse energy fluence from 7.1 to 14.1 J/m2, the dehybridization rates ranged from 0.002 to 0.05 DNA per pulse, and the total amount of DNA released into solution was controlled over a range of 26–93% in only 100 s of irradiation. By shifting the dsDNA position as little as ∼1.1 nm, the average dsDNA dehybridization rate is altered up to 30 ± 2%, providing a high level of control over DNA melting and release. By comparing the theoretical temperature around the dsDNA to the experimentally derived temperature, we find that maximum or peak temperatures have a greater influence on the dehybridization rate when the dsDNA is closer to the AuNP surface and when lower laser pulse fluences are used. Furthermore, molecular dynamics simulations mimicking the photothermal heat pulse around a AuNP provide mechanistic insight into the stochastic nature of dehybridization and demonstrate increased base pair separation near the AuNP surface during laser pulse heating when compared to steady-state heating. Understanding how biological materials respond to the short-lived and non-uniform temperature increases innate to fs laser pulse optical heating of AuNPs is critical to improving the functionality and precision of this technique so that it may be implemented into more complex biological systems.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Mechanistic Understanding of DNA Denaturation in Nanoscale Thermal Gradients Created by Femtosecond Excitation of Gold Nanoparticles

Hastman

Chaturvedi

et al. 2022

ACS Appl. Mater. Interfaces

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show abstract

“…Besides these protein biomarkers, nucleic acids as the neurological disorder biomarkers have also attracted more and more attention [97]. In allowing for early diagnosis [98][99][100][101], these promising methods may improve the treatability, quality of life, and outcomes for individuals with neurological disorders.…”

Section: Plasmonic Nanoprobes For Acute Neurological Disorder Biomark...mentioning

confidence: 99%

Recent progress in plasmonic nanoparticle‐based biomarker detection and cytometry for the study of central nervous system disorders

Peng

Jeong

et al. 2021

Cytometry Pt A

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Neurological disorders affect hundreds of millions of people around the world, are often life-threatening, untreatable, and can result in debilitating symptoms. The high prevalence of these disorders, which feature biochemical or structural abnormalities in neuronal systems, has spurned innovations in both rapid and early detection to assist in the selection of appropriate treatment strategies to improve the patients' quality of life. Plasmonic nanoparticles (PNPs), a versatile and promising class of nanomaterials, are widely utilized in numerous imaging techniques, drug delivery systems, and biomarker detection methods. Recently, PNP-based nanoprobes have attracted considerable attention for the early diagnosis of neurological disorders.

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“…Nanomaterial-mediated photothermal effects have attracted a lot of attention due to the unique light-to-heat conversion property, invasiveness, and compatibility in many biological systems. − Photothermal effects have been extensively studied in photothermal cancer therapy for more than two decades, but they have not been explored for biosensing until recently. ,− For example, our group developed the first photothermal immunoassay for the detection of cancer biomarkers using a thermometer, avoiding the need for expensive analytical instruments . Most recently, by applying gold nanoparticle aggregates as photothermal probes, another quantitative biosensing platform was developed for genetic analysis based on the gold nanoparticle aggregation-induced photothermal effects .…”

Section: Introductionmentioning

confidence: 99%

Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers

Zhou

2021

Anal. Chem.

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The volumetric bar-chart microfluidic chips (V-Chips) driven by chemical reaction-generated gas provide a promising platform for point-of-care (POC) visual biomarker quantitation. However, multiple limitations are encountered in conventional V-Chips, such as costly and complex chip fabrication, complicated assembly, and imprecise controllability of gas production. Herein, we introduced nanomaterial-mediated photothermal effects to V-Chips, and for the first time developed a new type of V-Chip, photothermal bar-chart microfluidic chip (PT-Chip), for visual quantitative detection of biochemicals without any bulky and costly analytical instruments.Immunosensing signals were converted to visual readout signals via photothermal effects, the onchip bar-chart movements, enabling quantitative biomarker detection on a low-cost polymer hybrid PT-Chip with on-chip scale rulers. Four different human serum samples containing prostate-specific antigen (PSA) as a model analyte were detected simultaneously using the PT-Chip, with the limit of detection of 2.1 ng/mL, meeting clinical diagnostic requirements. Although no conventional signal detectors were used, it achieved comparable detection sensitivity to absorbance measurements with a microplate reader. The PT-Chip was further validated by testing human whole blood without the color interference problem, demonstrating good analytical performance of our method even in complex matrixes and thus the potential to fill a gap in current clinical diagnostics that is incapable of testing whole blood. This new PT-Chip driven by nanomaterial-mediated photothermal effects opens a new horizon of microfluidic platforms for instrument-free diagnostics at the point of care.

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Quantifying and Adjusting Plasmon‐Driven Nano‐Localized Temperature Field around Gold Nanorods for Nucleic Acids Amplification

Cited by 14 publications

References 43 publications

Mechanistic Understanding of DNA Denaturation in Nanoscale Thermal Gradients Created by Femtosecond Excitation of Gold Nanoparticles

Mechanistic Understanding of DNA Denaturation in Nanoscale Thermal Gradients Created by Femtosecond Excitation of Gold Nanoparticles

Recent progress in plasmonic nanoparticle‐based biomarker detection and cytometry for the study of central nervous system disorders

Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers

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