Quantifying Aflatoxin B1 in peanut oil using fabricating fluorescence probes based on upconversion nanoparticles

Abstract

Rare earth doped upconversion nanoparticles convert near-infrared excitation light into visible emission light. Compared to organic fluorophores and semiconducting nanoparticles, upconversion nanoparticles (UCNPs) offer high photochemical stability, sharp emission bandwidths, and large anti-Stokes shifts. Along with the significant light penetration depth and the absence of autofluorescence in biological samples under infrared excitation, these UCNPs have attracted more and more attention on toxin detection and biological labelling. Herein, the fluorescence probe based on UCNPs was developed for quantifying Aflatoxin B1 (AFB1) in peanut oil. Based on a specific immunity format, the detection limit for AFB1 under optimal conditions was obtained as low as 0.2ng·ml(-1), and in the effective detection range 0.2 to 100ng·ml(-1), good relationship between fluorescence intensity and AFB1 concentration was achieved under the linear ratios up to 0.90. Moreover, to check the feasibility of these probes on AFB1 measurements in peanut oil, recovery tests have been carried out. A good accuracy rating (93.8%) was obtained in this study. Results showed that the nanoparticles can be successfully applied for sensing AFB1 in peanut oil.

The Control of Aflatoxin Contamination at Harvest, Drying, Pre‐ Storage and Storage Periods in Peanut: The New Approach

“…In recent years, with the rapid development of nanostructured materials and nanotechnology in the fields of biotechnology and contaminant detection, magnetic nanoparticles (MNPs) have been receiving considerable attention. In their research, Sun et al [91] used artificial antigen-modified MNPs employed as immune sensing probes, and antibody functionalised UCNPs were used as signal probes. Besides in this study, the antibodies-functionalised UCNPs were linked to the surface of the MNPs by antibody-antigen affinity [91].…”

“…In their research, Sun et al [91] used artificial antigen-modified MNPs employed as immune sensing probes, and antibody functionalised UCNPs were used as signal probes. Besides in this study, the antibodies-functionalised UCNPs were linked to the surface of the MNPs by antibody-antigen affinity [91]. According to Sun and co-workers, rare earth-doped upconversion nanoparticles were used successfully to assemble for sensing Aflatoxins B 1 in actual food samples (peanut oil) [91].…”

“…Besides in this study, the antibodies-functionalised UCNPs were linked to the surface of the MNPs by antibody-antigen affinity [91]. According to Sun and co-workers, rare earth-doped upconversion nanoparticles were used successfully to assemble for sensing Aflatoxins B 1 in actual food samples (peanut oil) [91]. Ezekiel et al [92] described a reliable and simple analytical method for the determination of aflatoxins (AFB 1 , AFB 2 , AFG 1 and AFG 2 ) in cereals, peanuts, vegetable oils and fermented foods such as beer, soybean sauce and soybean paste based on immunoaffinity column (IAC) cleanup coupled with direct competitive enzymelinked immunosorbent assay (dcELISA) detection and confirmed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) in their research.…”

Highly sensitive fluorescence sensing of zearalenone using a novel aptasensor based on upconverting nanoparticles

“…X-ray diffraction (XRD) measurements were performed to analyze the crystal structure of nanoparticles using an X-ray diffractometer (D2 PHASER, Bruker AXS Ltd., Germany) following the method reported by Hu, Sheng, Zhang, Wu, and Wang (2015). The surface groups of the bionanoparticles were identified by a FTIR spectrometer (Nicolet iS10, Thermo Electron Corp., Madison, WI, USA) in accordance with the procedure described by Sun, Li, Koidis, and Chen (2016). FTIR spectra was recorded in the range of 500-4000cm -1 with 256 scans at a resolution of 4 cm -1 .…”

“…Due to their appealing photo-physical properties, such as low autofluorescence background, good photochemical stability, large Stokes shifts nonblinking and nonbleaching emission, tunable fluorescence wavelength, deep penetration into biological samples, and relatively low toxicity, UCNPs are regarded as more promising fluorophores compare to traditional fluorescent material and have been used in medical field (Tang, Gao, Wang, Gao, Gao, Ma, et al, 2015;Wu, Xu, Li, Long, Jiao, Jin, et al, 2017). Recently, UCNPs have been successfully applied for the determination of mycotoxins, antibiotics and bacteria (Hu, Sheng, Zhang, Wang, Wu, & Wang, 2016;Pan, Zhao, & Chen, 2015;Sun, Li, Koidis, & Chen, 2016). To the best of our knowledge, no such research has been conducted to determine ZEN contents in food products using UCNPs.…”