Quantification ofAspergiullus flavus BFE96 by sybr green Q-PCR in black pepper

Bagnara, A.; Mayer, Zsuzsanna; Geisen, Rolf

doi:10.1007/bf02940049

Cited by 9 publications

(3 citation statements)

References 6 publications

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“…The other approach is to enhance fungal cell wall disruption prior to DNA extraction. This was achieved by thoroughly mixing the sample material with alumina in a lysis buffer (4,16,20). As with the liquid nitrogen method, in some PCR assays additional bands appeared and the specific PCR products were rather weak (16,20).…”

Section: Dna Isolationmentioning

confidence: 99%

The application of PCR in the detection of mycotoxigenic fungi in foods

Konietzny¹,

Greiner²

2003

Braz. J. Microbiol.

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It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene-as well as fumonisin-producing Fusaria strains have been described. The usefulness of the PCR methods developed so far to monitor quality and safety in the food an feed industry was already demonstrated. Thus, PCR may be applied to the screening of agricultural commodities for the absence of mycotoxin producers prior to or even after processing. Negative results in this assay indicate that a sample should be virtually free of mycotoxins. Only the positive samples left must be analyzed for the presence of mycotoxins using physicochemical standard methods. This review does not only summarize the so far developed qualitative and quantitative PCR assays for the detection of mycotoxigenic fungi in agricultural commodities, foods and animal feeds, but describes also strategies to develop new specific PCR assays for such a detection.

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Section: Dna Isolationmentioning

confidence: 99%

The application of PCR in the detection of mycotoxigenic fungi in foods

Konietzny¹,

Greiner²

2003

Braz. J. Microbiol.

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“…Mayer et al (2003b) demonstrated that the tendency of nor-1 gene copies was the same as that of A. flavus CFUs in wheat with the prolonged incubation time (Mayer et al, 2003b). Additionally, nor-1 copies were demonstrated to be correlated to CFUs of A. flavus in pepper, maize, and paprika (Bagnara et al, 2000;Mayer et al, 2003a). Passone et al (2010) also developed an analytical method determining Aspergillus section Flavi based on nor-1 gene and demonstrated a good correlation (r = 0.613; P < 0.0001) between nor-1 copies and CFUs in naturally stored peanut.…”

Section: Matrix Effect Sensitivity and Specificity For Total Aflatoxi...mentioning

confidence: 93%

Aspergillus-Derived Mycotoxins In The Feed And Food Chain

2021

Frontiers Research Topics

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Frontiers is more than just an open-access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers Journal SeriesThe Frontiers Journal Series is a multi-tier and interdisciplinary set of open-access, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers Journal Series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to QualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation.

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“…Additionally, nor-1 copies were demonstrated to be correlated to CFUs of A. flavus in pepper, maize, and paprika (Bagnara et al, 2000;Mayer et al, 2003a). Passone et al (2010) also developed an analytical method determining Aspergillus section Flavi based on nor-1 gene and demonstrated a good correlation (r = 0.613; P < 0.0001) between nor-1 copies and CFUs in naturally stored peanut.…”

Section: Matrix Effect Sensitivity and Specificity For Aspergillus mentioning

confidence: 95%

Small Molecular Contaminant and Microorganism Can Be Simultaneously Detected Based on Nanobody-Phage: Using Carcinogen Aflatoxin and Its Main Fungal Aspergillus Section Flavi spp. in Stored Maize for Demonstration

et al. 2020

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Simultaneous detection technology has become a hot topic in analytical chemistry; however, very few reports on how to simultaneously detect small molecular contaminants and microorganisms have been in place. Aflatoxins are a group of highly toxic and carcinogenic compounds, which are produced mainly by Aspergillus flavus and Aspergillus parasiticus from section Flavi responsible for aflatoxin accumulation in stored cereals. Both aflatoxins and Aspergillus section Flavi were used to demonstrate the duplex real-time RCR method of simultaneously detecting small molecular contaminants and microorganisms. The detection of aflatoxins and Aspergillus section Flavi was carried out depending on the anti-idiotypic nanobody-phage V 2−5 and aflatoxin-synthesis related gene nor-1 (=aflD), respectively. The quantitative standard curves for simultaneous detection of aflatoxins and Aspergillus section Flavi were constructed, with detection limits of 0.02 ng/ml and 8 × 10 2 spores/g, respectively. Naturally contaminated maize samples (n = 25) were analyzed for a further validation. The results were in good agreement between the new developed method and the referential methods (high-performance liquid chromatography and the conventional plating counts).

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Quantification ofAspergiullus flavus BFE96 by sybr green Q-PCR in black pepper

Cited by 9 publications

References 6 publications

The application of PCR in the detection of mycotoxigenic fungi in foods

The application of PCR in the detection of mycotoxigenic fungi in foods

Aspergillus-Derived Mycotoxins In The Feed And Food Chain

Small Molecular Contaminant and Microorganism Can Be Simultaneously Detected Based on Nanobody-Phage: Using Carcinogen Aflatoxin and Its Main Fungal Aspergillus Section Flavi spp. in Stored Maize for Demonstration

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