Quantification of Y chromosome bearing spermatozoa of cattle using in situ hybridization

Schwerin, Manfred; Blöttner, S.; Thomsen, Preben D.; Roschlau, Daniela; Brockmann, Gudrun A.

doi:10.1002/mrd.1080300106

Cited by 32 publications

(18 citation statements)

References 18 publications

(22 reference statements)

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“…In livestock species, the spermFISH technique was formerly used to quantify X-and Y-bearing sperm in cattle [17,28,31,36] and pigs [23], as well as for the estimation of aneuploidy rates in pigs [34]. We used it for the first time to estimate the proportion of unbalanced gametes in the semen of boars carrying different chromosomal rearrangements, and to predict their effects on reproduction.…”

Section: Introductionmentioning

confidence: 99%

Estimation of the proportion of genetically unbalanced spermatozoa in the semen of boars carrying chromosomal rearrangements using FISH on sperm nuclei

2004

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-Many chromosomal rearrangements are detected each year in France on young boars candidates for reproduction. The possible use of these animals requires a good knowledge of the potential effect of the rearrangements on the prolificacy of their mates. This effect can be estimated by an accurate determination of the rate of unbalanced spermatozoa in the semen of boars which carry the rearrangements. Indeed, these spermatozoa exhibiting normal fertilizing ability are responsible for an early embryonic mortality, and then, for a decrease of the litter sizes. The "spermFISH" technique, i.e. fluorescent in situ hybridization on decondensed sperm heads, has been used on several occasions in Man, in this perspective. In livestock species, this method was formerly used mainly for semen sexing purposes. We used it, for the first time, to estimate the rates of imbalance in the semen of four boars carrying chromosomal rearrangements: two reciprocal translocations, rcp(3;15)(q27;q13) and rcp(12;14)(q13;q21), as well as two independent cases of trisomy 18 mosaicism. The rates of unbalanced gametes were relatively high for the two reciprocal translocations (47.83% and 24.33%, respectively). These values differed from the apparent effects of the rearrangements estimated using a limited number of litters: a decrease in prolificacy of 23% (estimation obtained using the results of 6 litters) and 39% (57 litters), respectively for the 3/15 and 12/14 translocations. The imbalance rates were much lower for the trisomy mosaics (0.58% and 1.13%), suggesting a very moderate effect of this special kind of chromosomal rearrangement.

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Section: Introductionmentioning

confidence: 99%

Estimation of the proportion of genetically unbalanced spermatozoa in the semen of boars carrying chromosomal rearrangements using FISH on sperm nuclei

2004

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“…Reaction A consisted in the simultaneous amplification of two Y-chromosome specific segments using primer pairs 1 (forward 5'-CCTCCCCTTGTTCAAACGCCCGGAATCATT-3' and reverse 5'-TGCTTGACTGCAGGGACCGAGAGGTTTGGG-3') and 2 (forward 5'-ATCAGTGCAGGGACCGAGATG-3' and reverse 5'-AAGCAGCCGATAAACACTCCTT-3'). Primer pairs 1 and 2 amplified a 210 bp [19]and a 250 bp [20] product in bovine males, respectively; reaction B, by its turn, amplified a 280 bp product from a repetitive sequence of the bovine genome used as internal control (primer pair 3: 5'-AGGTCGCGAGATTGGTCGCTAGGTCATGCA-3' and 5'-AAGACCTCGAGAGACCCTCTTCAACACGT-3';) [21]. Thus, female samples produced only one band of 280 bp resulted from the amplification reaction B while male samples produced 3 bands of 280 bp and 250 bp (reaction A) and of 210 bp (reaction B).…”

Section: Determination Of Embryo Genetic Sex By Pcrmentioning

confidence: 99%

Enrichment of Bovine Semen with X-Bearing Spermatozoa Using Percoll? and Optiprep? Discontinuous Gradients

Lima¹

2015

AVS

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“…In livestock species, the sperm FISH technique was formerly used to quantify X-and Y-bearing sperms in cattle (Schwerin et al, 1991;Hassanane et al, 1999;Piumi et al, 2001;Rens et al, 2001) and pigs (Kawarasaki et al, 1998;Parrilla et al, 2003), as well as for the estimation of aneuploidy rates in pigs (Rubes et al, 1999). In this study the frequencies of disomies for chromosomes 1, 10, and Y (0.075%, 0.067%, and 0.094%, respectively) as well as of diploidies (0.177% for 1-1, 10-10 and 0.022% for Y-Y, 10-10) were estimated.…”

Section: Analysis Of the Meiotic Productsmentioning

confidence: 99%

Chromosomal abnormalities, meiotic behavior and fertility in domestic animals

Villagómez

Pinton²

2008

Cytogenet Genome Res

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Since the advent of the surface microspreading technique for synaptonemal complex analysis, increasing interest in describing the synapsis patterns of chromosome abnormalities associated with fertility of domestic animals has been noticed during the past three decades. In spite of the number of scientific reports describing the occurrence of structural chromosome abnormalities, their meiotic behavior and gametic products, little is known in domestic animal species about the functional effects of such chromosome aberrations in the germ cell line of carriers. However, some interesting facts gained from recent and previous studies on the meiotic behavior of chromosome abnormalities of domestic animals permit us to discuss, in the frame of recent knowledge emerging from mouse and human investigations, the possible mechanism implicated in the well known association between meiotic disruption and chromosome pairing failure. New cytogenetic techniques, based on molecular and immunofluorescent analyses, are allowing a better description of meiotic processes, including gamete production. The present communication reviews the knowledge of the meiotic consequences of chromosome abnormalities in domestic animals.

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Quantification of Y chromosome bearing spermatozoa of cattle using in situ hybridization

Cited by 32 publications

References 18 publications

Estimation of the proportion of genetically unbalanced spermatozoa in the semen of boars carrying chromosomal rearrangements using FISH on sperm nuclei

Estimation of the proportion of genetically unbalanced spermatozoa in the semen of boars carrying chromosomal rearrangements using FISH on sperm nuclei

Enrichment of Bovine Semen with X-Bearing Spermatozoa Using Percoll? and Optiprep? Discontinuous Gradients

Chromosomal abnormalities, meiotic behavior and fertility in domestic animals

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