Quantification of tube topography on micrographs of fluorescent staining of multi-layers cell culture. v1

Abstract

The induction or inhibition of angiogenesis can be assessed in vitro by the differentiation of endothelial cells. Indeed, these cells are capable of forming structures resembling a capillary network in culture. This approach is known as Endothelial Tube Formation Assay (ETFA). This type of analysis is traditionally performed on a low confluence monolayer of cells to assay the effect of soluble compounds on the formation of tubes. However, the modulation of tube formation by cell-cell interactions requires the co-culture of cells that leads to the creation of a multi-layer micro-tissue. Additionally, the identification of cells in the micro-tissue requires fluorescent staining resulting in discontinuities in the signal. EXTERNAL LINK 1 08/06/2020 C ita tio n : C ita tio n : Bertrand-David Segard (08/06/2020). Quantification of tube topography on micrographs of fluorescent staining of multi-layers cell culture..